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RCB2767 : NIH/3T3  update : 2018/08/02
細胞特性(Comment:英)Mouse fibroblast-like cell line. TKG0299 (Deposited from Tohoku Univ.).
細胞特性(日)マウス線維芽細胞様細胞株。 TKG0299 (東北大学医用細胞資源センターからの寄託)。
使用条件(英)Basically, there is no restriction regarding academic use.
提供申込書類 依頼書C-0001.pdf   同意書(非営利)C-0003.pdf   同意書(営利)C-0003p.pdf  
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Fukuda, Hiroshi
樹立者 Todaro, G. J.
寄託日 2008
元の細胞 TKG0299
動物種 mouse < Mammals
動物系統名 NIH Swiss
採取組織 embryo
細胞分類 other
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0299)
細胞寿命 infinite
細胞形態 fibroblast-like
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% CS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 3 x 10^(5) cells/60 mm dish
継代・培地交換頻度 Subculture : once/3-4 days
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 15件
利用者成果(日) 0件

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