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RCB2767 : NIH/3T3  update : 2018/08/02
CommentMouse fibroblast-like cell line. TKG0299 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Todaro, G. J.
Year of deposit 2008
Original cell TKG0299
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0299)
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 15

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298  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  Jainchill, J L, Aaronson, S A, Todaro, G J  J Virol  1969  4:549-53  PubMed ID: 4311790
5004  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Benveniste RE, Lieber MM, Todaro GJ.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531

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User's Publication
9971  Induction of G2/M arrest and apoptosis through mitochondria pathway by a dimer sesquiterpene lactone from Smallanthus sonchifolius in HeLa cells.  Kitai Y, Zhang X, Hayashida Y, Kakehi Y, Tamura H.  J Food Drug Anal  2017  25:619-627  PubMed ID: 28911648
10009  Illegitimate translation causes unexpected gene expression from on-target out-of-frame alleles created by CRISPR-Cas9.  Makino S, Fukumura R, Gondo Y.  Sci Rep  2016  6:39608  PubMed ID: 28000783
10786  Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures.  He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K.  Sci Rep  2018  8:4556  PubMed ID: 29540810
11126  Electrodeposition of Zwitterionic PEDOT Films for Conducting and Antifouling Surfaces.  Goda T, Miyahara Y.  Langmuir  2019  35(5):1126-1133  PubMed ID: 30001621
11165  Bioinspired Load-Bearing Hydrogel Based on Engineered Sea Anemone Skin-Derived Collagen-Like Protein.  Yang YJ, Choi YS, Cha HJ.  Biotechnol J  2018  13(12):e1800086  PubMed ID: 30102020
11362  Induced Proton Perturbation for Sensitive and Selective Detection of Tight Junction Breakdown.  Hatano H, Goda T, Matsumoto A, Miyahara Y.  Anal. Chem.  2019    PubMed ID: 30596415
11398  Self-Assembled Reduced Albumin and Glycol Chitosan Nanoparticles for Paclitaxel Delivery.  Razi MA, Wakabayashi R, Goto M, Kamiya N.  Langmuir  2019    PubMed ID: 30673276
11440  Exosome-mediated horizontal gene transfer occurs in double-strand break repair during genome editing.  Ono R, Yasuhiko Y, Aisaki KI, Kitajima S, Kanno J, Hirabayashi Y.  Commun Biol  2019    PubMed ID: 30775458
11479  Low Electric Treatment activates Rho GTPase via Heat Shock Protein 90 and Protein Kinase C for Intracellular Delivery of siRNA.  Hasan M, Hama S, Kogure K.  Sci Rep  2019    PubMed ID: 30858501
4432  Three-dimensional printed microfluidic modules for design changeable coaxial microfluidic devices    Sensors and Actuators B: Chemical  2018  274, 491-500 
11541  Synthesis, characterization, and liposome partition of a novel tetracycline derivative using the ionic liquids framework.  Alves F, Oliveira FS, Schröder B, Matos C, Marrucho IM.  J Pharm Sci  2013    PubMed ID: 23450634
11543  Switching of Cell Proliferation/Differentiation in Thiol-Maleimide Clickable Microcapsules Triggered by in Situ Conjugation of Biomimetic Peptides.  Oki Y, Kirita K, Ohta S, Ohba S, Horiguchi I, Sakai Y, Ito T.  Biomacromolecules  2019    PubMed ID: 31059241
11580  Sox9 regulates the luminal stem/progenitor cell properties of salivary glands.  Tanaka J, Mabuchi Y, Hata K, Yasuhara R, Takamatsu K, Kujiraoka S, Yukimori A, Takakura I, Sumimoto H, Fukada T, Azuma M, Akiyama H, Nishimura R, Shimane T, Mishima K.  Exp. Cell Res.  2019    PubMed ID: 31153924
11684  In Situ Fabrication of Double-Layered Hydrogels via Spray Processes to Prevent Postoperative Peritoneal Adhesion  Pan Qi, Ying Grace Zheng, Seiichi Ohta, Norihiro Kokudo, Kiyoshi Hasegawa, Taichi Ito  ACS Biomaterials Science & Engineering  2019   
11773  Bongkrekic acid facilitates glycolysis in cultured cells and induces cell death under low glucose conditions.  Kano A, Iwasaki T, Shindo M.  Biochem Biophys Rep  2019    PubMed ID: 31517068

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