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Cell No. : Cell Name
RCB2745 : A20  update : 2018/08/02
CommentMouse cell line derived from lymphoma. Balb/c strain. TKG0571 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2008
Original cell TKG0571
Animal mouse < Mammals
Strain name BALB/c
Tissue lymphoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0571)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 4
User's Publication 1


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Reference
5257  Kim KJ, Finnegan A.  Induction of IgG2a secretion from mIgG2a+ B-lymphoid tumor cells by BCDF present in several antigen-specific T-helper clones.  Cell Immunol  1987  110(1):149-62  PubMed ID: 2960459
5256  Kim KJ, Dejoy SQ.  Con A induction of IgG secretion from a B-lymphoid tumour cell line, A20.  Immunology  1986  59(1):15-21  PubMed ID: 3530982
5255  Laskov R1, Ishay-Michaeli R, Wallach M, Givol D, Kim KJ.  Simultaneous expression of mu- and gamma-chain mRNA in cloned murine B-lymphoma cell lines.  EMBO J  1983  2(2):167-72  PubMed ID: 11894921
5254  Kim KJ, Kanellopoulos-Langevin C, Merwin RM, Sachs DH, Asofsky R.  Establishment and characterization of BALB/c lymphoma lines with B cell properties.  J Immunol  1979  122(2):549-54  PubMed ID: 310843

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User's Publication
11275  Tanaka M, Ishige A, Yaguchi M, Matsumoto T, Shirouzu M, Yokoyama S, Ishikawa F, Kitabayashi I, Takemori T, Harada M.  Development of a simple new flow cytometric antibody-dependent cellular cytotoxicity (ADCC) assay with excellent sensitivity.  J. Immunol. Methods  2019    PubMed ID: 30389576



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