CELL SEARCH SYSTEM -CELL BANK- (RIKEN BRC) [RCB2710 : Atg5^(+/+)MEF]

細胞材料の再培養方法 ( HPS以外・ HPS )

細胞番号 : 細胞名
RCB2710 : Atg5^(+/+)MEF update : 2024/11/08

細胞特性(Comment:英)

Mouse embryonic fibroblasts. Wild type control for RCB2711.

細胞特性(日)

マウス胎児由来線維芽細胞。RCB2711の比較対照野生型細胞。

細胞特性(寄託者記述:英)

細胞特性(寄託者記述:日)

使用条件(英)

1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (Nature 2004;432;1032-1036) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.

使用条件(日)

1) 非営利団体による学術研究に限る。 2) 利用者は事前に寄託者の提供承諾書を得ること。 3) 当該リソースの非営利団体の営利目的での利用および営利団体の利用については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。 4) 研究成果の公表にあたって寄託者の指定する文献 (Nature 2004;432;1032-1036) を引用すること。 5) 研究成果の公表にあたって謝辞の表明を必要とする。 6) 利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。

備考(英)

備考(日)

承諾者住所

提供申込書類(英)

Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf)

Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).

提供申込書類(日)

依頼書C-0001.pdf 承諾書C-0002.pdf 同意書(非営利学術目的)C-0003.pdf

提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。

提供手数料

手数料とお支払いについてはこちらをご覧ください。

細胞基本情報

寄託者

Mizushima, Noboru

樹立者

Mizushima, Noboru

寄託日

2007

別名

Atg5WT_A

動物種

_mouse < Mammals

動物系統名

C57BL/6x129sv

性別

Unknown

齢

embryo

採取組織

embryo/fetus, whole

病名

wild type (ATG gene +/+)

細胞分類

transformed

遺伝子改変

recombinant

外来遺伝子

SV40 large T

細胞寿命

infinite

細胞形態

fibroblast-like

Cellosaurus(Expasy)

CVCL_0J74

細胞培養・検査情報

寄託時情報

ロット情報

培地・試薬情報

培地・試薬一覧はこちらをご覧ください。

培養形態

Adherent cells

培地

DMEM (low glucose) + 10% FBS

抗生物質

Free

継代方法

0.25% Trypsin

継代密度

1 : 6-10 split

継代・培地交換頻度

Subculture or Medium Renewal : once/2-3 days

培養最適温度

37 ℃

二酸化炭素濃度

5 %

凍結培地

Medium + 10% DMSO

凍結方法

Slow freezing

マイコプラズマ/アコレプラズマ

(-)

マウス系統検査

アイソザイム検査

LD, NP

画像情報

寄託時情報

ロット情報

文献情報

Reference(英)

2件

Reference(日)

0件

利用者成果(英)

44件

利用者成果(日)

0件

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Reference(英)

8452 Mizushima N, Yoshimori T, Levine B. Methods in mammalian autophagy research Cell 2010 140(3):313-26 PubMed ID: 20144757 DOI: 10.1016/j.cell.2010.01.028

3621 Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N. The role of autophagy during the early neonatal starvation period. Nature 2004 432(7020):1032-6 PubMed ID: 15525940 DOI: 10.1038/nature03029

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Reference(日)

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利用者成果(英)

22050 Annagrazia Adornetto, Maria Luisa Laganà, Andrea Satriano, Ester Licastro, Maria Tiziana Corasaniti, Giacinto Bagetta, Rossella Russo The Antidepressant Drug Amitriptyline Affects Human SH-SY5Y Neuroblastoma Cell Proliferation and Modulates Autophagy International Journal of Molecular Sciences 2924 25:10415 DOI: 10.3390/ijms251910415

17739 Hung CH, Yen JB, Chang PJ, Chen LW, Huang TY, Tsai WJ, Tsai YC. Characterization of Human Norovirus Nonstructural Protein NS1.2 Involved in the Induction of the Filamentous Endoplasmic Reticulum, Enlarged Lipid Droplets, LC3 Recruitment, and Interaction with NTPase and NS4 Viruses 2023 15(3):812 PubMed ID: 36992520 DOI: 10.3390/v15030812

19944 Aihara S, Torisu K, Uchida Y, Imazu N, Nakano T, Kitazono T. Spermidine from arginine metabolism activates Nrf2 and inhibits kidney fibrosis Commun Biol 2023 6(1):676 PubMed ID: 37380734 DOI: 10.1038/s42003-023-05057-w

21118 Aki T, Kukita M, Takata M, Funakoshi T, Unuma K, Uemura K. Induction of filamin-C and its involvement in the regulation of cellular senescence and apoptosis in Huh-7 hepatoma cells during arsenic trioxide exposure. Biochem Biophys Res Commun 2023 651:92-97 PubMed ID: 36801614 DOI: 10.1016/j.bbrc.2023.02.020

20691 Kajiwara K, Osaki H, Greßies S, Kuwata K, Kim JH, Gensch T, Sato Y, Glorius F, Yamaguchi S, Taki M. A negative-solvatochromic fluorescent probe for visualizing intracellular distributions of fatty acid metabolites. Nat Commun 2022 13(1):2533 PubMed ID: 35534485 DOI: 10.1038/s41467-022-30153-6

18096 Qiao Y, Choi JE, Tien JC, Simko SA, Rajendiran T, Vo JN, Delekta AD, Wang L, Xiao L, Hodge NB, Desai P, Mendoza S, Juckette K, Xu A, Soni T, Su F, Wang R, Cao X, Yu J, Kryczek I, Wang XM, Wang X, Siddiqui J, Wang Z, Bernard A, Fernandez-Salas E, Navone NM, Ellison SJ, Ding K, Eskelinen EL, Heath EI, Klionsky DJ, Zou W, Chinnaiyan AM. Autophagy Inhibition by Targeting PIKfyve Potentiates Response to Immune Checkpoint Blockade in Prostate Cancer Nat Cancer 2021 2:978-993 PubMed ID: 34738088 DOI: 10.1038/s43018-021-00237-1

20339 Sharma KB, Chhabra S, Aggarwal S, Tripathi A, Banerjee A, Yadav AK, Vrati S, Kalia M. Proteomic landscape of Japanese encephalitis virus-infected fibroblasts. J Gen Virol 2021 PubMed ID: 34546869 DOI: 10.1099/jgv.0.001657

16432 Sarkar R, Sharma KB, Kumari A, Asthana S, Kalia M. Japanese encephalitis virus capsid protein interacts with non-lipidated MAP1LC3 on replication membranes and lipid droplets J Gen Virol 2021 Jan;102(1) PubMed ID: 33095129 DOI: 10.1099/jgv.0.001508

16981 Uchida Y, Torisu K, Ueki K, Tsuruya K, Nakano T, Kitazono T. Autophagy gene ATG7 regulates albumin transcytosis in renal tubule epithelial cells Am J Physiol Renal Physiol 2021 321(5):F572-F586 PubMed ID: 34541900 DOI: 10.1152/ajprenal.00172.2021

11865 Xu ZD, Wang Y, Liang G, Liu ZQ, Ma WH, Chu CT, Wei HF. Propofol affects mouse embryonic fibroblast survival and proliferation in vitro via ATG5- and calcium-dependent regulation of autophagy. Acta Pharmacol. Sin. 2020 41(3):303-310 PubMed ID: 31645660 DOI: 10.1038/s41401-019-0303-z

13223 Liu EA, Schultz ML, Mochida C, Chung C, Paulson HL, Lieberman AP. Fbxo2 mediates clearance of damaged lysosomes and modifies neurodegeneration in the Niemann-Pick C brain. JCI Insight 2020 PubMed ID: 32931479 DOI: 10.1172/jci.insight.136676

15153 Hitomi K, Okada R, Loo TM, Miyata K, Nakamura AJ, Takahashi A. DNA Damage Regulates Senescence-Associated Extracellular Vesicle Release via the Ceramide Pathway to Prevent Excessive Inflammatory Responses Int J Mol Sci 2020 21(10):3720 PubMed ID: 32466233 DOI: 10.3390/ijms21103720

11371 Aki T, Unuma K, Noritake K, Hirayama N, Funakoshi T, Uemura K. Formation of high molecular weight p62 by CORM-3. PLoS ONE 2019 PubMed ID: 30620762 DOI: 10.1371/journal.pone.0210474

11463 Iwasawa T, Shinomiya T, Ota N, Shibata N, Nakata K, Shiina I, Nagahara Y. Novel Ridaifen-B Structure Analog Induces Apoptosis and Autophagy Depending on Pyrrolidine Side Chain. Biol. Pharm. Bull. 2019 PubMed ID: 30828072 DOI: 10.1248/bpb.b18-00643

11727 Ji CH, Kim HY, Heo AJ, Lee SH, Lee MJ, Kim SB, Srinivasrao G, Mun SR, Cha-Molstad H, Ciechanover A, Choi CY, Lee HG, Kim BY, Kwon YT. The N-Degron Pathway Mediates ER-phagy. Mol. Cell 2019 PubMed ID: 31375263 DOI: 10.1016/j.molcel.2019.06.028

6908 Ko PJ, Woodrow C, Dubreuil MM, Martin BR, Skouta R, Bassik MC, Dixon S. A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death. Cell Chem Biol 2019 26(12):1716-1724 PubMed ID: 31631010 DOI: 10.1016/j.chembiol.2019.09.014

9181 Huiyin Lan, Mingjia Tan, Qiang Zhang, Fei Yang, Siyuan Wang, Hua Li, Xiufang Xiong, Yi Sun LSD1 Destabilizes FBXW7 and Abrogates FBXW7 Functions Independent of Its Demethylase Activity Proc Natl Acad Sci U S A 2019 116 (25), 12311-12320 PubMed ID: 31152129 DOI: 10.1073/pnas.1902012116

11079 Sharma M, Sharma KB, Chauhan S, Bhattacharyya S, Vrati S, Kalia M. Diphenyleneiodonium enhances oxidative stress and inhibits Japanese encephalitis virus induced autophagy and ER stress pathways. Biochem. Biophys. Res. Commun. 2018 502(2):232-237 PubMed ID: 29792860 DOI: 10.1016/j.bbrc.2018.05.149

4148 Aki T, Unuma K, Noritake K, Kurahashi H, Funakoshi T, Uemura K. Interaction of carbon monoxide-releasing ruthenium carbonyl CORM-3 with plasma fibronectin. Toxicol In Vitro 2018 PubMed ID: 29580987 DOI: 10.1016/j.tiv.2018.03.010

9173 Alexa R Wilden, Joshua A Molina, Melissa Feuerborn, Daniel Boyle, Stella Y Lee Glutamine-dependent Lysosome Homeostatic Changes Induced by Starvation and Lysosome Inhibition Biochim Biophys Acta Mol Cell Res 2018 1865 (9), 1356-1367 PubMed ID: 29966622 DOI: 10.1016/j.bbamcr.2018.06.014

15047 Real E, Rodrigues L, Cabal GG, Enguita FJ, Mancio-Silva L, Mello-Vieira J, Beatty W, Vera IM, Zuzarte-Luís V, Figueira TN, Mair GR, Mota MM. Plasmodium UIS3 sequesters host LC3 to avoid elimination by autophagy in hepatocytes Nat Microbiol 2018 3(1):17-25 PubMed ID: 29109477 DOI: 10.1038/s41564-017-0054-x

9185 Hyunjoo Cha-Molstad, Ji Eun Yu, Zhiwei Feng, Su Hyun Lee, Jung Gi Kim, Peng Yang, Bitnara Han, Ki Woon Sung, Young Dong Yoo, Joonsung Hwang, Terry McGuire, Sang Mi Shim, Hyun Dong Song, Srinivasrao Ganipisetti 1, Nuozhou Wang, Jun Min Jang, Min Jae Lee, Seung Jun Kim, Kyung Ho Lee 1, Jin Tae Hong, Aaron Ciechanover , Inhee Mook-Jung, Kwang Pyo Kim, Xiang-Qun Xie, Yong Tae Kwon, Bo Yeon Kim p62/SQSTM1/Sequestosome-1 Is an N-recognin of the N-end Rule Pathway Which Modulates Autophagosome Biogenesis Nat Commun 2017 8 (1), 102 PubMed ID: 28740232 DOI: 10.1038/s41467-017-00085-7

14213 Into T, Horie T, Inomata M, Gohda J, Inoue JI, Murakami Y, Niida S. Basal autophagy prevents autoactivation or enhancement of inflammatory signals by targeting monomeric MyD88 Sci Rep 2017 7(1):1009 PubMed ID: 28432355 DOI: 10.1038/s41598-017-01246-w

14756 Kawano S, Torisu T, Esaki M, Torisu K, Matsuno Y, Kitazono T. Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion Biol Open 2017 6(11):1644-1653 PubMed ID: 28970230 DOI: 10.1242/bio.027458

9158 Nguyen T Tien, Ilker Karaca , Irfan Y Tamboli, Jochen Walter Trehalose Alters Subcellular Trafficking and the Metabolism of the Alzheimer-associated Amyloid Precursor Protein J Biol Chem 2016 291 (20), 10528-40 PubMed ID: 26957541 DOI: 10.1074/jbc.M116.719286

9164 Bhavya B Chandrika, Cheng Yang, Yang Ou, Xiaoke Feng, Djamali Muhoza, Alexandrea F Holmes, Sue Theus, Sarika Deshmukh, Randy S Haun, Gur P Kaushal Endoplasmic Reticulum Stress-Induced Autophagy Provides Cytoprotection From Chemical Hypoxia and Oxidant Injury and Ameliorates Renal Ischemia-Reperfusion Injury PLoS One 2015 10 (10), e0140025 PubMed ID: 26444017 DOI: 10.1371/journal.pone.0140025

15041 Cha-Molstad H, Sung KS, Hwang J, Kim KA, Yu JE, Yoo YD, Jang JM, Han DH, Molstad M, Kim JG, Lee YJ, Zakrzewska A, Kim SH, Kim ST, Kim SY, Lee HG, Soung NK, Ahn JS, Ciechanover A, Kim BY, Kwon YT. Amino-terminal arginylation targets endoplasmic reticulum chaperone BiP for autophagy through p62 binding Nat Cell Biol 2015 17(7):917-29 PubMed ID: 26075355 DOI: 10.1038/ncb3177

15211 Unuma K, Aki T, Funakoshi T, Hashimoto K, Uemura K. Extrusion of mitochondrial contents from lipopolysaccharide-stimulated cells: Involvement of autophagy Autophagy 2015 11(9):1520-36 PubMed ID: 26102061 DOI: 10.1080/15548627.2015.1063765

9111 Shintaro Kobayashi, Yasuko Orba, Hiroki Yamaguchi, Kenta Takahashi, Michihito Sasaki, Rie Hasebe, Takashi Kimura, Hirofumi Sawa Autophagy Inhibits Viral Genome Replication and Gene Expression Stages in West Nile Virus Infection Virus Res 2014 191, 83-91 PubMed ID: 25091564 DOI: 10.1016/j.virusres.2014.07.016

9137 Isabelle Hamer, Emeline Goffin, Xavier De Bolle, Jean-Jacques Letesson, Michel Jadot Replication of Brucella Abortus and Brucella Melitensis in Fibroblasts Does Not Require Atg5-dependent Macroautophagy BMC Microbiol 2014 14, 223 PubMed ID: 25179110 DOI: 10.1186/s12866-014-0223-5

9184 Bo-Jhih Guan, Dawid Krokowski, Mithu Majumder, Christine L Schmotzer, Scot R Kimball, William C Merrick, Antonis E Koromilas, Maria Hatzoglou Translational Control During Endoplasmic Reticulum Stress Beyond Phosphorylation of the Translation Initiation Factor eIF2α J Biol Chem 2014 289 (18), 12593-611 PubMed ID: 24648524 DOI: 10.1074/jbc.M113.543215

15871 McLelland GL, Soubannier V, Chen CX, McBride HM, Fon EA. Parkin and PINK1 function in a vesicular trafficking pathway regulating mitochondrial quality control EMBO J 2014 33(4):282-95 PubMed ID: 24446486 DOI: 10.1002/embj.201385902

16009 Sharma M, Bhattacharyya S, Nain M, Kaur M, Sood V, Gupta V, Khasa R, Abdin MZ, Vrati S, Kalia M. Japanese encephalitis virus replication is negatively regulated by autophagy and occurs on LC3-I- and EDEM1-containing membranes Autophagy 2014 10(9):1637-51 PubMed ID: 25046112 DOI: 10.4161/auto.29455

9165 Hiroko Inoue, Katsunori Hase, Arata Segawa, Tsuyoshi Takita H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulphonamide) Induces Autophagy Independently of Protein Kinase A Inhibition Eur J Pharmacol 2013 714 (1-3), 170-7 PubMed ID: 23810683 DOI: 10.1016/j.ejphar.2013.06.018

9168 Shintaro Kobayashi, Tadaki Suzuki, Manabu Igarashi, Yasuko Orba, Noriko Ohtake, Keita Nagakawa, Kenichi Niikura, Takashi Kimura, Harumi Kasamatsu, Hirofumi Sawa Cysteine Residues in the Major Capsid Protein, Vp1, of the JC Virus Are Important for Protein Stability and Oligomer Formation PLoS One 2013 8 (10), e76668 PubMed ID: 24130786 DOI: 10.1371/journal.pone.0076668

9150 Yutaka Ueda, Fan-Yan Wei, Taku-ichiro Hide, Hiroyuki Michiue, Kentaro Takayama, Taku Kaitsuka, Hideo Nakamura, Keishi Makino, Jun-ichi Kuratsu, Shiroh Futaki, Kazuhito Tomizawa Induction of Autophagic Cell Death of Glioma-Initiating Cells by Cell-Penetrating D-isomer Peptides Consisting of Pas and the p53 C-terminus Biomaterials 2012 33 (35), 9061-9 PubMed ID: 23006589 DOI: 10.1016/j.biomaterials.2012.09.003

9153 Satoshi Tsuyuki, Sayuri Fukui, Akari Watanabe, Satoshi Akune, Mizuki Tanabe, Kenichi Yoshida Delphinidin Induces Autolysosome as Well as Autophagosome Formation and Delphinidin-Induced Autophagy Exerts a Cell Protective Role J Biochem Mol Toxicol 2012 26 (11), 445-53 PubMed ID: 23129091 DOI: 10.1002/jbt.21443

9154 Kai Er Eng, Marc D Panas, Deirdre Murphy, Gunilla B Karlsson Hedestam, Gerald M McInerney Accumulation of Autophagosomes in Semliki Forest Virus-Infected Cells Is Dependent on Expression of the Viral Glycoproteins J Virol 2012 86 (10), 5674-85 PubMed ID: 22438538 DOI: 10.1128/JVI.06581-11

7903 Tian Y, Bustos V, Flajolet M, Greengard P. A small-molecule enhancer of autophagy decreases levels of Abeta and APP-CTF via Atg5-dependent autophagy pathway. FASEB J. 2011 25:1934-42 PubMed ID: 21368103 DOI: 10.1096/fj.10-175158

7963 Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J. Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation. J. Neurosci. 2011 31:1837-49 PubMed ID: 21289194 DOI: 10.1523/JNEUROSCI.2954-10.2011

9148 Chengqun Huang, Allen M Andres, Eric P Ratliff, Genaro Hernandez, Pamela Lee, Roberta A Gottlieb Preconditioning Involves Selective Mitophagy Mediated by Parkin and p62/SQSTM1 PLoS One 2011 6 (6), e20975 PubMed ID: 21687634 DOI: 10.1371/journal.pone.0020975

9152 Natura Myeku, Maria E Figueiredo-Pereira Dynamics of the Degradation of Ubiquitinated Proteins by Proteasomes and Autophagy: Association With Sequestosome 1/p62 J Biol Chem 2011 286 (25), 22426-40 PubMed ID: 21536669 DOI: 10.1074/jbc.M110.149252

9163 Amy Colleran 1, Aideen Ryan, Angela O'Gorman, Coralie Mureau, Catherine Liptrot, Peter Dockery, Howard Fearnhead, Laurence J Egan Autophagosomal IkappaB Alpha Degradation Plays a Role in the Long Term Control of Tumor Necrosis Factor-Alpha-Induced Nuclear factor-kappaB (NF-kappaB) Activity J Biol Chem 2011 286 (26), 22886-93 PubMed ID: 21454695 DOI: 10.1074/jbc.M110.199950

8269 Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM. A novel quantitative flow cytometry-based assay for autophagy. Autophagy 2010 6:634-41 PubMed ID: 20458170 DOI: 10.4161/auto.6.5.12112

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