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RCB2710 : Atg5^(+/+)MEF  update : 2018/07/06
CommentMouse embryonic fibroblasts. Wild type control for RCB2711.
Comment from the depositor
Terms and conditions1) BIOLOGICAL RESOURCE is limited to for academic researches in non-profit organization. 2) The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 3) Any use of the BIOLOGICAL RESOURCE for profit purposes by a non-profit organization or any use of the BIOLOGICAL RESOURCE by a profit organization requires a separate license from the DEPOSITOR prior to distribution. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the original publication (Nature 2004;432;1032-1036) designated by the DEPOSITOR is required. 5) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 6) The RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use with the results from the use of BIOLOGICAL RESOURCE.
approver's address
The University of Tokyo, Graduate School of Medicine
Department of Biochemistry and Molecular Biology,Mizushima Lab.
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033 Japan
Dr.Mizushima, Noboru
Fax. +81-3-3815-1490
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Mizushima, Noboru
Originator Mizushima, Noboru
Year of deposit 2007
Another name Atg5WT_A
Animal mouse < Mammals
Strain name C57BL/6x129sv
Gender Unknown
Age at sampling embryo
Tissue embryo/fetus, whole
Case history wild type (ATG gene +/+)
Classification transformed
Recombinant recombinant
Exogene SV40 large T
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 6-10 split
SC frequency Subculture or Medium Renewal : once/2-3 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 1
User's Publication 9

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3621  Kuma A, Hatano M, Matsui M, Yamamoto A, Nakaya H, Yoshimori T, Ohsumi Y, Tokuhisa T, Mizushima N.  The role of autophagy during the early neonatal starvation period.  Nature  2004  432(7020):1032-6  PubMed ID: 15525940

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User's Publication
11371  Aki T, Unuma K, Noritake K, Hirayama N, Funakoshi T, Uemura K.  Formation of high molecular weight p62 by CORM-3.  PLoS ONE  2019    PubMed ID: 30620762
11463  Iwasawa T, Shinomiya T, Ota N, Shibata N, Nakata K, Shiina I, Nagahara Y.  Novel Ridaifen-B Structure Analog Induces Apoptosis and Autophagy Depending on Pyrrolidine Side Chain.  Biol. Pharm. Bull.  2019    PubMed ID: 30828072
11727  Ji CH, Kim HY, Heo AJ, Lee SH, Lee MJ, Kim SB, Srinivasrao G, Mun SR, Cha-Molstad H, Ciechanover A, Choi CY, Lee HG, Kim BY, Kwon YT.  The N-Degron Pathway Mediates ER-phagy.  Mol. Cell  2019    PubMed ID: 31375263
6908  Ko PJ, Woodrow C, Dubreuil MM, Martin BR, Skouta R, Bassik MC, Dixon S.  A ZDHHC5-GOLGA7 Protein Acyltransferase Complex Promotes Nonapoptotic Cell Death.  Cell Chem Biol  2019  26(12):1716-1724  PubMed ID: 31631010
11079  Sharma M, Sharma KB, Chauhan S, Bhattacharyya S, Vrati S, Kalia M.  Diphenyleneiodonium enhances oxidative stress and inhibits Japanese encephalitis virus induced autophagy and ER stress pathways.  Biochem. Biophys. Res. Commun.  2018  502(2):232-237  PubMed ID: 29792860
4148  Aki T, Unuma K, Noritake K, Kurahashi H, Funakoshi T, Uemura K.  Interaction of carbon monoxide-releasing ruthenium carbonyl CORM-3 with plasma fibronectin.  Toxicol In Vitro  2018    PubMed ID: 29580987
7903  Tian Y, Bustos V, Flajolet M, Greengard P.  A small-molecule enhancer of autophagy decreases levels of Abeta and APP-CTF via Atg5-dependent autophagy pathway.  FASEB J.  2011  25:1934-42  PubMed ID: 21368103
7963  Tamboli IY, Hampel H, Tien NT, Tolksdorf K, Breiden B, Mathews PM, Saftig P, Sandhoff K, Walter J.  Sphingolipid storage affects autophagic metabolism of the amyloid precursor protein and promotes Abeta generation.  J. Neurosci.  2011  31:1837-49  PubMed ID: 21289194
8269  Eng KE, Panas MD, Karlsson Hedestam GB, McInerney GM.  A novel quantitative flow cytometry-based assay for autophagy.  Autophagy  2010  6:634-41  PubMed ID: 20458170

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