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RCB2700 : PK-8  update : 2018/08/02
CommentHuman cell line derived from liver metastasis of pancreas carcinoma. TKG0383 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Kobari, Masao
Year of deposit 2007
Original cell TKG0383
Animal human < Mammals
Genus Homo
Species sapiens
Tissue pancreas, liver meta
Case history pancreas carcinoma
Metastastatic ability Yes
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0383)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 8


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Reference
5253  Establishment of six human pancreatic cancer cell lines and their sensitivities to anti-tumor drugs.  Kobari M, Hisano H, Matsuno S, Sato T, Kan M, Tachibana T.  Tohoku J Exp Med  1986  150(3):231-48  PubMed ID: 3547771

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User's Publication
7044  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  Am J Cancer Res  2017  6:2799-2815  PubMed ID: 28042501
9938  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  BMC Genomics  2016  17:899  PubMed ID: 27829362
10750  Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1β from activated macrophages leads to poor prognosis in pancreatic cancer.  Arima K, Komohara Y, Bu L, Tsukamoto M, Itoyama R, Miyake K, Uchihara T, Ogata Y, Nakagawa S, Okabe H, Imai K, Hashimoto D, Chikamoto A, Yamashita YI, Baba H, Ishimoto T.  Cancer Sci.  2018  109:462-470  PubMed ID: 29224225
11218  Inhibition of 15-PGDH causes Kras-driven tumor expansion through prostaglandin E2-ALDH1 signaling in the pancreas.  Arima K, Ohmuraya M, Miyake K, Koiwa M, Uchihara T, Izumi D, Gao F, Yonemura A, Bu L, Okabe H, Imai K, Hashimoto D, Baba Y, Chikamoto A, Yamashita YI, Furukawa T, Araki K, Baba H, Ishimoto T.  Oncogene  2018    PubMed ID: 30250298
5030  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611
6031  Active Secretion of Dimerized S100A11 Induced by the Peroxisome in Mesothelioma Cells.  Saho S et al  Cancer Microenviron  2016  9(2-3):93-105 
11820  MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer.  Okazaki J, Tanahashi T, Sato Y, Miyoshi J, Nakagawa T, Kimura T, Miyamoto H, Fujino Y, Nakamura F, Takehara M, Ma B, Bando M, Kitamura S, Okamoto K, Muguruma N, Sogabe M, Takayama T.  Digestion  2019    PubMed ID: 31563901
11862  Method to Convert Stem Cells into Cancer Stem Cells.  Afify SM, Chen L, Yan T, Calle AS, Nair N, Murakami C, Zahra MH, Okada N, Iwasaki Y, Seno A, Seno M.  Methods Protoc  2019    PubMed ID: 31426391



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