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RCB2700 : PK-8  update : 2018/08/02
CommentHuman cell line derived from liver metastasis of pancreas carcinoma. TKG0383 (Deposited from Tohoku Univ.).
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Kobari, Masao
Year of deposit 2007
Original cell TKG0383
Animal human < Mammals
Genus Homo
Species sapiens
Tissue pancreas, liver meta
Case history pancreas carcinoma
Metastastatic ability Yes
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0383)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 6


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Reference

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User's Publication
Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2017  6:2799-2815  PubMed ID: 28042501
Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
Arima K, Komohara Y, Bu L, Tsukamoto M, Itoyama R, Miyake K, Uchihara T, Ogata Y, Nakagawa S, Okabe H, Imai K, Hashimoto D, Chikamoto A, Yamashita YI, Baba H, Ishimoto T.  Downregulation of 15-hydroxyprostaglandin dehydrogenase by interleukin-1β from activated macrophages leads to poor prognosis in pancreatic cancer.  Cancer Sci.  2018  109:462-470  PubMed ID: 29224225
  NOVEL BISPHOSPHONIC ACID DERIVATIVE AND APPLICATION FOR SAME        PubMed ID:
Arima K, Ohmuraya M, Miyake K, Koiwa M, Uchihara T, Izumi D, Gao F, Yonemura A, Bu L, Okabe H, Imai K, Hashimoto D, Baba Y, Chikamoto A, Yamashita YI, Furukawa T, Araki K, Baba H, Ishimoto T.  Inhibition of 15-PGDH causes Kras-driven tumor expansion through prostaglandin E2-ALDH1 signaling in the pancreas.  Oncogene  2018    PubMed ID: 30250298



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