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RCB2638 : B16/BL6  update : 2018/08/02
CommentMouse cell line derived from melanoma.  TKG0598 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Year of deposit 2007
Original cell TKG0598
Animal mouse < Mammals
Strain name C57BL/6
Tissue skin
Case history melanoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0598)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 5
User's Publication 21

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5219  In vitro selection of murine B16 melanoma variants with enhanced tissue-invasive properties.  Poste G, Doll J, Hart IR, Fidler IJ.  Cancer Res  1980  40(5):1636-44  PubMed ID: 7370995
5246  Involvement of macrophages in the eradication of established metastases following intravenous injection of liposomes containing macrophage activators.  Fidler IJ, Barnes Z, Fogler WE, Kirsh R, Bugelski P, Poste G.  Cancer Res  1982  42(2):496-501  PubMed ID: 7055801
5247  Comparison of the metastatic properties of B16 melanoma clones isolated from cultured cell lines, subcutaneous tumors, and individual lung metastases.  Poste G, Doll J, Brown AE, Tzeng J, Zeidman I.  Cancer Res  1982  42(7):2770-8  PubMed ID: 7083167
5248  Biochemical regulation of adenylate cyclase in murine melanoma clones with different metastatic properties.  Sheppard JR, Lester B, Doll J, Buscarino C, Gonzales E, Corwin S, Greig R, Poste G.  Int J Cancer  1986  37(5):713-22  PubMed ID: 3009332
5249  Macrophage content of spontaneous metastases at different stages of growth.  Bugelski PJ, Corwin SP, North SM, Kirsh RL, Nicolson GL, Poste G.  Cancer Res  1987  47(15):4141-5  PubMed ID: 3300960

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User's Publication
7158  Isosakuranetin, a 4'-O-methylated flavonoid, stimulates melanogenesis in B16BL6 murine melanoma cells.  Drira R, Sakamoto K.  Life Sci.  2015  143:43-9  PubMed ID: 26524968
7656  Host-derived MMP-13 exhibits a protective role in lung metastasis of melanoma cells by local endostatin production.  Fukuda H, Mochizuki S, Abe H, Okano HJ, Hara-Miyauchi C, Okano H, Yamaguchi N, Nakayama M, D'Armiento J, Okada Y.  Br. J. Cancer  2011  105:1615-24  PubMed ID: 22015555
7721  The antitumor effect of a novel angiogenesis inhibitor (an octahydronaphthalene derivative) targeting both VEGF receptor and NF-κB pathway.  Watari K, Nakamura M, Fukunaga Y, Furuno A, Shibata T, Kawahara A, Hosoi F, Kuwano T, Kuwano M, Ono M.  Int. J. Cancer  2011  131:310-21  PubMed ID: 21826646
9556  NAD(P)H dehydrogenase, quinone 1 (NQO1), protects melanin-producing cells from cytotoxicity of rhododendrol.  Okubo A, Yasuhira S, Shibazaki M, Takahashi K, Akasaka T, Masuda T, Maesawa C.  Pigment Cell Melanoma Res  2016  29:309-16  PubMed ID: 26847926
9861  Exosome-based tumor antigens-adjuvant co-delivery utilizing genetically engineered tumor cell-derived exosomes with immunostimulatory CpG DNA.  Morishita M, Takahashi Y, Matsumoto A, Nishikawa M, Takakura Y.  Biomaterials  2016  111:55-65  PubMed ID: 27723556
10305  Anti-tumor effects of differentiation-inducing factor-1 in malignant melanoma: GSK-3-mediated inhibition of cell proliferation and GSK-3-independent suppression of cell migration and invasion.  Arioka M, Takahashi-Yanaga F, Kubo M, Igawa K, Tomooka K, Sasaguri T.  Biochem. Pharmacol.  2017  138:31-48  PubMed ID: 28501501
10680  Carnosic acid, an inducer of NAD(P)H quinone oxidoreductase 1, enhances the cytotoxicity of β-lapachone in melanoma cell lines.  Arakawa N, Okubo A, Yasuhira S, Takahashi K, Amano H, Akasaka T, Masuda T, Shibazaki M, Maesawa C.  Oncol Lett  2018  15:2393-2400  PubMed ID: 29434949
10730  Role of Extracellular Vesicle Surface Proteins in the Pharmacokinetics of Extracellular Vesicles.  Charoenviriyakul C, Takahashi Y, Morishita M, Nishikawa M, Takakura Y.  Mol. Pharm.  2018  15:1073-1080  PubMed ID: 29382201
10745  Verification of radiodynamic therapy by medical linear accelerator using a mouse melanoma tumor model.  Takahashi J, Murakami M, Mori T, Iwahashi H.  Sci Rep  2018  8:2728  PubMed ID: 29426920
10814  In Vivo Antitumor Effects of MK615 Led by PD-L1 Downregulation.  Yanaki M, Kobayashi M, Aruga A, Nomura M, Ozaki M.  Integr Cancer Ther  2018  17(3):646-653  PubMed ID: 29665734
10831  A Novel Strategy to Increase the Yield of Exosomes (Extracellular Vesicles) for an Expansion of Basic Research.  Emam SE, Ando H, Abu Lila AS, Shimizu T, Ukawa M, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishida T.  Biol. Pharm. Bull.  2018  41:733-742  PubMed ID: 29709910
11141  Antimetastatic Effects of Carbon-Ion Beams on Malignant Melanomas.  Matsumoto Y, Furusawa Y, Uzawa A, Hirayama R, Koike S, Ando K, Tsuboi K, Sakurai H.  Radiat. Res.  2018  190(4):412-423  PubMed ID: 30040045
11224  Liposome co-incubation with cancer cells secreted exosomes (extracellular vesicles) with different proteins expressions and different uptake pathways.  Emam SE, Ando H, Lila ASA, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Sagawa I, Ishida T.  Sci Rep  2018    PubMed ID: 30262875
11248  Preservation of exosomes at room temperature using lyophilization.  Charoenviriyakul C, Takahashi Y, Nishikawa M, Takakura Y.  Int J Pharm  2018    PubMed ID: 30316791
11311  Macrophage-mediated transfer of cancer-derived components to stromal cells contributes to establishment of a pro-tumor microenvironment.  Umakoshi M, Takahashi S, Itoh G, Kuriyama S, Sasaki Y, Yanagihara K, Yashiro M, Maeda D, Goto A, Tanaka M.  Oncogene  2019    PubMed ID: 30459356
11317  Effects of 7 T static magnetic fields on the expression of biological markers and the formation of bone in rats.  Yamaguchi-Sekino S, Kira T, Sekino M, Akahane M.  Bioelectromagnetics  2019    PubMed ID: 30466173
4155  Periostin antisense oligonucleotide suppresses bleomycin-induced formation of a lung premetastatic niche for melanoma.  Semba T, Sugihara E, Kamoshita N, Ueno S, Fukuda K, Yoshino M, Takao K, Yoshikawa K, Izuhara K, Arima Y, Suzuki M, Saya H.  Cancer Sci.  2018    PubMed ID: 29498146
4156  C-X-C Motif Ligand 1 (CXCL1) from melanoma cells down-regulates the invasion of their metastatic melanoma cells.  Hatano T, Yashiro M, Fujikawa H, Motomura H.  Oncotarget  2018    PubMed ID: 30123429
11504  N-glycome inheritance from cells to extracellular vesicles in B16 melanomas.  Harada Y, Kizuka Y, Tokoro Y, Kondo K, Yagi H, Kato K, Inoue H, Taniguchi N, Maruyama I.  FEBS Lett.  2019    PubMed ID: 30943309
11822  Development of DNA-anchored assembly of small extracellular vesicle for efficient antigen delivery to antigen presenting cells.  Matsumoto A, Takahashi Y, Ariizumi R, Nishikawa M, Takakura Y.  Biomaterials  2019    PubMed ID: 31586864
11859  Cancer cell-type tropism is one of crucial determinants for the efficient systemic delivery of cancer cell-derived exosomes to tumor tissues.  Emam SE, Selim Abu Lila A, Elsadek NE, Ando H, Shimizu T, Okuhira K, Ishima Y, Mahdy MA, Ghazy FS, Ishid T.  Eur J Pharm Biopharm  2019    PubMed ID: 31629787

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