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RCB2623 : KLN205  update : 2018/08/02
CommentMouse cell line derived from lung squamous cell carcinoma. TKG0214 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Fukuda, Hiroshi
Originator Kaneko, T.
Year of deposit 2007
Original cell TKG0214 KLN205(CRL1453)
Animal mouse < Mammals
Strain name DBA/2
Tissue lung
Case history squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0214)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 0.1mM NEAA
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 2

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5197  Kaneko T, LePage GA, Shnitka TK.  KLN205--a murine lung carcinoma cell line.  In Vitro  1980  16(10):884-92  PubMed ID: 7419233
5196  Kaneko T, LePage GA.  Growth characteristics and drug responses of a murine lung carcinoma in vitro and in vivo.  Cancer Res  1978  38(7):2084-90  PubMed ID: 418873

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User's Publication
11603  Aoki N, Matsui Y.  Comprehensive Analysis of Mouse Cancer/Testis Antigen Functions in Cancer Cells and Roles of TEKT5 in Cancer Cells and Testicular Germ Cells.  Mol. Cell. Biol.  2019    PubMed ID: 31208979
10011  Suzawa K, Shien K, Peng H, Sakaguchi M, Watanabe M, Hashida S, Maki Y, Yamamoto H, Tomida S, Soh J, Asano H, Tsukuda K, Nasu Y, Kumon H, Miyoshi S, Toyooka S.  Distant Bystander Effect of REIC/DKK3 Gene Therapy Through Immune System Stimulation in Thoracic Malignancies.  Anticancer Res.  2017  37:301-307  PubMed ID: 28011506

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