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RCB2403 : MG6  update : 2018/07/06
CommentMouse microglial cell line immortalized by a replication-deficient retroviral vector containing human c-myc gene. No significant production of infectious viral particles from MG6 cells was confirmed by bioassay using SC-1 cells.
Comment from the depositor
Use conditionb
Use condition description1) A permission of the approver is required. 2) If any intellectual Property is generated during the use of the delivered Material, the Recipient shall consult with the Administrator of the Material at the NARO. 3) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Neurosci Lett 2006;407:205-210, Biochim Biophys Acta 2005;1726:177-186 ) designated by the DEPOSITOR is required.
approver's address
×
English
Address
The National Agriculture and Food Research Organization
Institute of Agrobiological Sciences, Division of Animal Science
•1-2 Owashi, Tsukuba, Ibaraki 305-8634 Japan
Dr.Kitani, Hiroshi
Fax. +81-29-838-6043
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kitani, Hiroshi
Originator Kitani, Hiroshi
Year of deposit 2006
Another name MG6-1
Animal mouse < Mammals
Strain name C57BL/6
Gender Unknown
Age at sampling 1 day
Tissue brain
Classification transformed
Recombinant recombinant
Exogene human c-myc, neomycin, retroviral vector
Lifespan infinite
Morphology other (macrophage-like)
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (high glucose) + 10% FBS + 10μg/ml Insulin + 0.1mM 2-Mercaptoethanol
Antibiotics Free
Passage method pipetting
Coating dish Non-tissue culture dish
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 19


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Reference

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User's Publication
Wu Z, Ni J, Liu Y, Teeling JL, Takayama F, Collcutt A, Ibbett P, Nakanishi H.  Cathepsin B plays a critical role in inducing Alzheimer's disease-like phenotypes following chronic systemic exposure to lipopolysaccharide from Porphyromonas gingivalis in mice.  Brain Behav. Immun.  2017  65:350-361  PubMed ID: 28610747
Hosain MZ, Mori T, Kishimura A, Katayama Y.  Synergy between phenotypic modulation and ROS neutralization in reduction of inflammatory response of hypoxic microglia by using phosphatidylserine and antioxidant containing liposomes.  J Biomater Sci Polym Ed  2015  27:290-302  PubMed ID: 26689775
Ni J, Wu Z, Peterts C, Yamamoto K, Qing H, Nakanishi H.  The Critical Role of Proteolytic Relay through Cathepsins B and E in the Phenotypic Change of Microglia/Macrophage.  J. Neurosci.  2015  35:12488-501  PubMed ID: 26354916
Takenouchi T, Tsukimoto M, Iwamaru Y, Sugama S, Sekiyama K, Sato M, Kojima S, Hashimoto M, Kitani H.  Extracellular ATP induces unconventional release of glyceraldehyde-3-phosphate dehydrogenase from microglial cells.  Immunol. Lett.  2015  167:116-24  PubMed ID: 26277554
Segawa S, Tatsumi N, Ohishi A, Nishida K, Nagasawa K.  Characterization of zinc uptake by mouse primary cultured astrocytes and microglia.  Metallomics  2015  7:1067-77  PubMed ID: 25924210
Kitajima H, Komizu Y, Ueoka R.  Hybrid liposomes inhibit growth and invasion of human osteosarcoma cells leading to apoptosis.  Bioorg. Med. Chem. Lett.  2012  22:1784-7  PubMed ID: 22260774
Yanagitai M, Itoh S, Kitagawa T, Takenouchi T, Kitani H, Satoh T.  Carnosic acid, a pro-electrophilic compound, inhibits LPS-induced activation of microglia.  Biochem. Biophys. Res. Commun.  2012  418:22-6  PubMed ID: 22214931
Takenouchi T, Iwamaru Y, Sugama S, Tsukimoto M, Fujita M, Sekigawa A, Sekiyama K, Sato M, Kojima S, Conti B, Hashimoto M, Kitani H.  The activation of P2X7 receptor induces cathepsin D-dependent production of a 20-kDa form of IL-1β under acidic extracellular pH in LPS-primed microglial cells.  J. Neurochem.  2011  117:712-23  PubMed ID: 21395581
Hossain MS, Mineno K, Katafuchi T.  Neuronal Orphan G-Protein Coupled Receptor Proteins Mediate Plasmalogens-Induced Activation of ERK and Akt Signaling.  PLoS ONE  2016  11:e0150846  PubMed ID: 26934370
Takayama F, Hayashi Y, Wu Z, Liu Y, Nakanishi H.  Diurnal dynamic behavior of microglia in response to infected bacteria through the UDP-P2Y6 receptor system.  Sci Rep  2016  6:30006  PubMed ID: 27445174
Kurinami H, Shimamura M, Nakagami H, Shimizu H, Koriyama H, Kawano T, Wakayama K, Mochizuki H, Rakugi H, Morishita R.  A Novel Therapeutic Peptide as a Partial Agonist of RANKL in Ischemic Stroke.  Sci Rep  2016  6:38062  PubMed ID: 27897273
Mahmoud ME, Fereig R, Nishikawa Y.  Involvement of Host Defense Mechanisms against Toxoplasma gondii Infection in Anhedonic and Despair-Like Behaviors in Mice.  Infect. Immun.  2017  85:  PubMed ID: 28138019
Inoue T, Tanaka M, Masuda S, Ohue-Kitano R, Yamakage H, Muranaka K, Wada H, Kusakabe T, Shimatsu A, Hasegawa K, Satoh-Asahara N.  Omega-3 polyunsaturated fatty acids suppress the inflammatory responses of lipopolysaccharide-stimulated mouse microglia by activating SIRT1 pathways.  Biochim. Biophys. Acta  2017  1862:552-560  PubMed ID: 28254441
Denehy TR, Choe JY, Gregori CA, Breen JL.  Modified Le Fort partial colpocleisis with Kelly urethral plication and posterior colpoperineoplasty in the medically compromised elderly: a comparison with vaginal hysterectomy, anterior colporrhaphy, and posterior colpoperineoplasty.  Am. J. Obstet. Gynecol.  1995  173:1697-701; discussion 1701-2  PubMed ID: 8610747
Liu Y, Wu Z, Nakanishi Y, Ni J, Hayashi Y, Takayama F, Zhou Y, Kadawaki T, Nakanishi H.  Infection of microglia with Porphyromonas gingivalis promotes cell migration and an inflammatory response through the gingipain-mediated activation of protease-activated receptor-2 in mice.  Sci Rep  2017  7:11759  PubMed ID: 28924232
Kidana K, Tatebe T, Ito K, Hara N, Kakita A, Saito T, Takatori S, Ouchi Y, Ikeuchi T, Makino M, Saido TC, Akishita M, Iwatsubo T, Hori Y, Tomita T.  Loss of kallikrein-related peptidase 7 exacerbates amyloid pathology in Alzheimer's disease model mice.  EMBO Mol Med  2018    PubMed ID: 29311134
Kawakami M, Hattori M, Ohashi W, Fujimori T, Hattori K, Takebe M, Tomita K, Yokoo H, Matsuda N, Yamazaki M, Hattori Y.  Role of G protein-coupled receptor kinase 2 in oxidative and nitrosative stress-related neurohistopathological changes in a mouse model of sepsis-associated encephalopathy.  J. Neurochem.  2018    PubMed ID: 29500815
  The Critical Role of IL-10 in the Anti-neuroinflammatory and Anti-oxidative Effects of Rheum tanguticum on Activated Microglia        PubMed ID:
Matsumoto S, Caliskan N, Rodnina MV, Murata A, Nakatani K.  Small synthetic molecule-stabilized RNA pseudoknot as an activator for -1 ribosomal frameshifting.  Nucleic Acids Res.  2018    PubMed ID: 30085309



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