Cell No. : Cell Name RCB2366 : Hu5/E18  update : 2023/10/16
Comment			Human multipotent stem cell line able to differentiate into muscle, bone, and adipocytes.
Comment from the depositor
Terms and conditions			1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2006;348:1383-1388, Mech Dev 2008;125：257-269) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hashimoto, Naohiro
		Originator	Hashimoto, Naohiro
		Year of deposit	2006
		Another name	E18
		Original cell	Hu5/E
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Race	Japanese
		Gender	Female
		Age at sampling	42 years
		Tissue	subcutaneous muscle
		Classification	semi-normal
		Recombinant	recombinant
		Exogene	hTERT, HPV16-E7
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_U895
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (high glucose) + 20% FBS + 2% Ultroser G
		Antibiotics		Free
		Passage method		0.25% Trypsin
		Coating dish		type I collagen coated dish
	Culture information	Passage cell No		2-4 x 10 5 cells/100 mm dish
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		10 %
		Freeze medium		90% FBS + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	4
		User's Publication	2

Top

Reference

15666  Shiomi K, Kiyono T, Okamura K, Uezumi M, Goto Y, Yasumoto S, Shimizu S, Hashimoto N.  CDK4 and cyclin D1 allow human myogenic cells to recapture growth property without compromising differentiation potential  Gene Ther  2011  18(9):857-66  PubMed ID: 21490680   DOI: 10.1038/gt.2011.44

5696  Hashimoto N, Kiyono T, Wada MR, Umeda R, Goto Y, Nonaka I, Shimizu S, Yasumoto S, Inagawa-Ogashiwa M.  Osteogenic properties of human myogenic progenitor cells.  Mech Dev  2008  125(3-4):257-69  PubMed ID: 18164186   DOI: 10.1016/j.mod.2007.11.004

5692  Hashimoto N, Kiyono T, Wada MR, Shimizu S, Yasumoto S, Inagawa M.  Immortalization of human myogenic progenitor cell clone retaining multipotentiality.  Biochem Biophys Res Commun  2006  348(4):1383-8  PubMed ID: 16919240   DOI: 10.1016/j.bbrc.2006.08.006

15658  Wada MR, Inagawa-Ogashiwa M, Shimizu S, Yasumoto S, Hashimoto N.  Generation of different fates from multipotent muscle stem cells  Development  2002  129(12):2987-95  PubMed ID: 12050145   DOI: 10.1242/dev.129.12.2987

Top

User's Publication

5693  Goto K, Imamura K, Komatsu K3, Mitani K, Aiba K, Nakatsuji N, Inoue M, Kawata A, Yamashita H, Takahashi R, Inoue H.  Simple Derivation of Spinal Motor Neurons from ESCs/iPSCs Using Sendai Virus Vectors.  Mol Ther Methods Clin Dev  2017  4:115-125  PubMed ID: 28344997   DOI: 10.1016/j.omtm.2016.12.007

5694  Tanaka A, Woltjen K, Miyake K, Hotta A, Ikeya M, Yamamoto T, Nishino T, Shoji E, Sehara-Fujisawa A, Manabe Y, Fujii N, Hanaoka K, Era T, Yamashita S, Isobe K, Kimura E, Sakurai H.  Efficient and reproducible myogenic differentiation from human iPS cells: prospects for modeling Miyoshi Myopathy in vitro.  PLoS One  2013  8(4):e61540  PubMed ID: 23626698   DOI: 10.1371/journal.pone.0061540