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RCB2354 : 293gp  update : 2018/07/06
CommentRetroviral vector packaging cell line expressing MoMLV (Moloney murine leukemia virus) gag/pol. Production of high titer retrovirus by cotransfection of envelope and vector plasmids.
Comment from the depositor
Terms and conditionsIn publishing the research results to be obtained by use of 293gp, an acknowledgment to Dr. N.V. Somia is requested.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Miyoshi, Hiroyuki
Year of deposit 2006
Original cell HEK293
Animal human < Mammals
Genus Homo
Species sapiens
Age at sampling embryo
Tissue kidney
Classification transformed
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
5184  Somia NV1, Miyoshi H, Schmitt MJ, Verma IM.  Retroviral vector targeting to human immunodeficiency virus type 1-infected cells by receptor pseudotyping.  J Virol  2000  74(9):4420-4  PubMed ID: 10756057

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User's Publication
7274  Tohmonda T, Yoda M, Iwawaki T, Matsumoto M, Nakamura M, Mikoshiba K, Toyama Y, Horiuchi K.  IRE1α/XBP1-mediated branch of the unfolded protein response regulates osteoclastogenesis.  J. Clin. Invest.  2015  125:3269-79  PubMed ID: 26193638



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