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RCB2351 : OGU1  update : 2018/08/02
CommentHuman cell line derived from primary effusion lymphoma (PEL). Application consideration
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kawauchi, Kiyotaka
Originator Kawauchi, Kiyotaka
Year of deposit 2006
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 76 years
Tissue ascites
Case history primary effusion lymphoma
Classification cancer
Year of origin 2000
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
7409  Kawauchi K, Ogasawara T, Aiba M, Fujibayashi M, Sanaka T, Sakura H, Shibuya M.  Establishment and characterization of a novel VEGF-producing HHV-8-unrelated PEL-like lymphoma cell line, OGU1.  Eur. J. Haematol.  2015  96:144-51  PubMed ID: 25846106
5181  Ogasawara T, Yasuyama M, Kawauchi K.  Constitutive activation of extracellular signal-regulated kinase and p38 mitogen-activated protein kinase in B-cell lymphoproliferative disorders.  Int J Hematol  2003  77(4):364-70  PubMed ID: 12774925

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