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Cell No. : Cell Name
RCB2342 : NCR-G2  update : 2024/01/17
CommentHuman cell line derived from embryonal carcinoma of testis.
Comment from the depositor
Terms and conditionsIn publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Acta Histchem. Cytochem.1992;25:562-576) .
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Hata, Jun-ichi
Originator Hata, Jun-ichi
Year of deposit 2006
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue testis
Disease name mixed embryonal carcinoma
Classification cancer
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_3060
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium G-031101 (with serum)
Culture method
Antibiotics within G-031101
Passage method pipetting
Culture information Passage ratio 1 : 2 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium : 2 x FM1 = 1 : 1 (or Medium + 10% DMSO)
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
STR(human) OK
Images
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lot info
Reference information Reference 3
User's Publication 0


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Reference
3617      Acta Histchem Cytochem  1992  25:562-576   
946  JUN-ICHI HATA, JUNICHIRO FUJIMOTO, EIZABURO ISHII, AKIHIRO UMEZAWA, YASUO KOKAI, YOSHIE MATSUBAYASHI, HITOSHI ABE, SATORU KUSAKARI, HARUTO KIKUCHI, TAKETO YAMADA, TATSUYA MARUYAMA  DIFFERENTIATION OF HUMAN GERM CELL TUMOR CELLS IN VIVO AND IN VITRO  ACTA HISTOCHEMICA ET CYTOCHEMICA  1992  25:563-576    DOI: 10.1267/ahc.25.563
948  J Hata, H Fujita, E Ikeda, Y Matsubayashi, Y Kokai, J Fujimoto  Differentiation of human germ cell tumor cells  Hum Cell  1989  2(4):382-7  PubMed ID: 2562098  

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