細胞番号 : 細胞名
RCB2326 : CHO-AA8
update : 2024/02/01
|
| 細胞特性(Comment:英) | A subline of the CHO-K1 cell line. Widely used for the isolation of repair-deficient mutants. Useful for gene mutation assays at the APRT and HPRT loci. |
| 細胞特性(日) | CHO-K1細胞亜株。UV、放射線感受性変異株の親株として広く使用。APRT、HPRT遺伝子座の変異頻度の解析に有用。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
|
|
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
|
| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
|
細胞基本情報
|
|
寄託者 |
Miyoshi, Hiroyuki
|
| 樹立者 |
Larry H. Thompson
|
| 寄託日 |
2006
|
| 動物種 |
_hamster
< Mammals
|
| 動物系統名 |
Chinese
|
| 属名 |
Cricetulus
|
| 種名 |
griseus
|
| 性別 |
Female
|
| 齢 |
adult
|
| 採取組織 |
ovary
|
| 細胞分類 |
mutant
|
|
細胞寿命 |
infinite
|
| 細胞形態 |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_4386
|
|
細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
|
培養形態 |
|
Adherent cells
|
| 培地 |
|
MEM ALPHA + 10% FBS
|
| 抗生物質 |
|
Free
|
|
継代方法 |
|
0.25% Trypsin
|
|
継代密度 |
|
1 : 8 split
|
| 継代・培地交換頻度 |
|
Subculture : 2 times/week
|
| 培養最適温度 |
|
37
℃
|
| 二酸化炭素濃度 |
|
5
%
|
| 凍結培地 |
|
Medium + 10% DMSO
|
| 凍結方法 |
|
Slow freezing
|
| マイコプラズマ |
|
(-)
|
| 動物種PCR |
|
OK
|
| アイソザイム検査 |
|
LD, NP
|
| 画像情報 |
寄託時情報 | ロット情報 |
|
|
|
| 文献情報 |
Reference(英) |
12件
|
| Reference(日) |
0件
|
| 利用者成果(英) |
3件
|
| 利用者成果(日) |
0件
|
| Reference(英) |
|
5160
Pergola F, Zdzienicka MZ, Lieber MR.
V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.
Mol Cell Biol
1993
13(6):3464-71
PubMed ID: 8497262
DOI: 10.1128/mcb.13.6.3464
|
|
5140
Fuller LF, Painter RB.
A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication.
Mutat Res
1988
193(2):109-21
PubMed ID: 3347204
DOI: 10.1016/0167-8817(88)90041-7
|
|
5129
Thompson LH, Brookman KW, Mooney CL.
Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene.
Somat Cell Mol Genet
1984
10(2):183-94
PubMed ID: 6584989
DOI: 10.1007/BF01534907
|
|
5130
Dillehay LE, Thompson LH, Carrano AV.
DNA-strand breaks associated with halogenated pyrimidine incorporation.
Mutat Res
1984
131(3-4):129-36
PubMed ID: 6717468
DOI: 10.1016/0167-8817(84)90052-x
|
|
5136
Chan JY, Thompson LH, Becker FF.
DNA-ligase activities appear normal in the CHO mutant EM9.
Mutat Res
1984
131(5-6):209-14.
PubMed ID: 6738565
DOI: 10.1016/0167-8817(84)90027-0
|
|
5131
MacInnes MA, Bingham JM, Thompson LH, Strniste GF.
DNA-mediated cotransfer of excision repair capacity and drug resistance into chinese hamster ovary mutant cell line UV-135.
Mol Cell Biol
1984
4(6):1152-8
PubMed ID: 6738535
DOI: 10.1128/mcb.4.6.1152
|
|
5132
La Belle M, Linn S, Thompson LH.
Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9.
Mutat Res
1984
141(1):41-4.
PubMed ID: 6482894
DOI: 10.1016/0165-7992(84)90035-6
|
|
5128
Dillehay LE, Thompson LH, Minkler JL, Carrano AV.
The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.
Mutat Res
1983
109(2):283-96
PubMed ID: 6843572
DOI: 10.1016/0027-5107(83)90053-2
|
|
5126
Thompson LH, Brookman KW, Dillehay LE, Carrano AV, Mazrimas JA, Mooney CL, Minkler JL.
A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
Mutat Res
1982
95(2-3):427-40
PubMed ID: 6889677
DOI: 10.1016/0027-5107(82)90276-7
|
|
5127
Thompson LH, Brookman KW, Dillehay LE, Mooney CL, Carrano AV.
Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions.
Somatic Cell Genet
1982
8(6):759-73
PubMed ID: 7163954
DOI: 10.1007/BF01543017
|
|
5124
Thompson LH, Rubin JS, Cleaver JE, Whitmore GF, Brookman K.
A screening method for isolating DNA repair-deficient mutants of CHO cells.
Somatic Cell Genet
1980
6(3):391-405.
PubMed ID: 7404270
DOI: 10.1007/BF01542791
|
|
5125
Thompson LH, Fong S, Brookman K.
Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells.
Mutat Res
1980
74(1):21-36
PubMed ID: 7360155
DOI: 10.1016/0165-1161(80)90188-0
|
| 利用者成果(英) |
|
12984
Kawanishi M, Yoneda R, Totsuka Y, Yagi T.
Genotoxicity of micro- and nano-particles of kaolin in human primary dermal keratinocytes and fibroblasts.
Genes Environ
2020
PubMed ID: 32322315
DOI: 10.1186/s41021-020-00155-1
|
|
11932
Kawanishi M, Hisatomi Y, Oda Y, Shimohara C, Tsunematsu Y, Sato M, Hirayama Y, Miyoshi N, Iwashita Y, Yoshikawa Y, Sugimura H, Mutoh M, Ishikawa H, Wakabayashi K, Yagi T, Watanabe K.
In vitro genotoxicity analyses of colibactin-producing E. coli isolated from a Japanese colorectal cancer patient.
J Toxicol Sci
2019
PubMed ID: 31813906
DOI: 10.2131/jts.44.871
|
|
15932
Hirayama R, Ito A, Noguchi M, Matsumoto Y, Uzawa A, Kobashi G, Okayasu R, Furusawa Y.
OH radicals from the indirect actions of X-rays induce cell lethality and mediate the majority of the oxygen enhancement effect
Radiat Res
2013
180:514-23
PubMed ID: 24138483
DOI: 10.1667/RR13368.1
|
戻る
細胞材料の再培養方法 (
HPS以外・
HPS )
ページトップへ
細胞材料開発室サイト
