Cell No. : Cell Name
RCB2326 : CHO-AA8
update : 2024/02/01
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| Comment | A subline of the CHO-K1 cell line. Widely used for the isolation of repair-deficient mutants. Useful for gene mutation assays at the APRT and HPRT loci. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Miyoshi, Hiroyuki
|
| Originator |
Larry H. Thompson
|
| Year of deposit |
2006
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Genus |
Cricetulus
|
| Species |
griseus
|
| Gender |
Female
|
| Age at sampling |
adult
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| Tissue |
ovary
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| Classification |
mutant
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|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_4386
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deposit info |
lot info |
|
Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM ALPHA + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| Animal PCR |
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OK
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| Isozyme |
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LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
12
|
| User's Publication |
3
|
| Reference |
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5160
Pergola F, Zdzienicka MZ, Lieber MR.
V(D)J recombination in mammalian cell mutants defective in DNA double-strand break repair.
Mol Cell Biol
1993
13(6):3464-71
PubMed ID: 8497262
DOI: 10.1128/mcb.13.6.3464
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5140
Fuller LF, Painter RB.
A Chinese hamster ovary cell line hypersensitive to ionizing radiation and deficient in repair replication.
Mutat Res
1988
193(2):109-21
PubMed ID: 3347204
DOI: 10.1016/0167-8817(88)90041-7
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5129
Thompson LH, Brookman KW, Mooney CL.
Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene.
Somat Cell Mol Genet
1984
10(2):183-94
PubMed ID: 6584989
DOI: 10.1007/BF01534907
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5130
Dillehay LE, Thompson LH, Carrano AV.
DNA-strand breaks associated with halogenated pyrimidine incorporation.
Mutat Res
1984
131(3-4):129-36
PubMed ID: 6717468
DOI: 10.1016/0167-8817(84)90052-x
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5136
Chan JY, Thompson LH, Becker FF.
DNA-ligase activities appear normal in the CHO mutant EM9.
Mutat Res
1984
131(5-6):209-14.
PubMed ID: 6738565
DOI: 10.1016/0167-8817(84)90027-0
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5131
MacInnes MA, Bingham JM, Thompson LH, Strniste GF.
DNA-mediated cotransfer of excision repair capacity and drug resistance into chinese hamster ovary mutant cell line UV-135.
Mol Cell Biol
1984
4(6):1152-8
PubMed ID: 6738535
DOI: 10.1128/mcb.4.6.1152
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5132
La Belle M, Linn S, Thompson LH.
Apurinic/apyrimidinic endonuclease activities appear normal in the CHO-cell ethyl methanesulfonate-sensitive mutant, EM9.
Mutat Res
1984
141(1):41-4.
PubMed ID: 6482894
DOI: 10.1016/0165-7992(84)90035-6
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5128
Dillehay LE, Thompson LH, Minkler JL, Carrano AV.
The relationship between sister-chromatid exchange and perturbations in DNA replication in mutant EM9 and normal CHO cells.
Mutat Res
1983
109(2):283-96
PubMed ID: 6843572
DOI: 10.1016/0027-5107(83)90053-2
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5126
Thompson LH, Brookman KW, Dillehay LE, Carrano AV, Mazrimas JA, Mooney CL, Minkler JL.
A CHO-cell strain having hypersensitivity to mutagens, a defect in DNA strand-break repair, and an extraordinary baseline frequency of sister-chromatid exchange.
Mutat Res
1982
95(2-3):427-40
PubMed ID: 6889677
DOI: 10.1016/0027-5107(82)90276-7
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5127
Thompson LH, Brookman KW, Dillehay LE, Mooney CL, Carrano AV.
Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions.
Somatic Cell Genet
1982
8(6):759-73
PubMed ID: 7163954
DOI: 10.1007/BF01543017
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5124
Thompson LH, Rubin JS, Cleaver JE, Whitmore GF, Brookman K.
A screening method for isolating DNA repair-deficient mutants of CHO cells.
Somatic Cell Genet
1980
6(3):391-405.
PubMed ID: 7404270
DOI: 10.1007/BF01542791
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5125
Thompson LH, Fong S, Brookman K.
Validation of conditions for efficient detection of HPRT and APRT mutations in suspension-cultured Chinese hamster ovary cells.
Mutat Res
1980
74(1):21-36
PubMed ID: 7360155
DOI: 10.1016/0165-1161(80)90188-0
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| User's Publication |
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12984
Kawanishi M, Yoneda R, Totsuka Y, Yagi T.
Genotoxicity of micro- and nano-particles of kaolin in human primary dermal keratinocytes and fibroblasts.
Genes Environ
2020
PubMed ID: 32322315
DOI: 10.1186/s41021-020-00155-1
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11932
Kawanishi M, Hisatomi Y, Oda Y, Shimohara C, Tsunematsu Y, Sato M, Hirayama Y, Miyoshi N, Iwashita Y, Yoshikawa Y, Sugimura H, Mutoh M, Ishikawa H, Wakabayashi K, Yagi T, Watanabe K.
In vitro genotoxicity analyses of colibactin-producing E. coli isolated from a Japanese colorectal cancer patient.
J Toxicol Sci
2019
PubMed ID: 31813906
DOI: 10.2131/jts.44.871
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15932
Hirayama R, Ito A, Noguchi M, Matsumoto Y, Uzawa A, Kobashi G, Okayasu R, Furusawa Y.
OH radicals from the indirect actions of X-rays induce cell lethality and mediate the majority of the oxygen enhancement effect
Radiat Res
2013
180:514-23
PubMed ID: 24138483
DOI: 10.1667/RR13368.1
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