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Cell No. : Cell Name
RCB2324 : MIH5(RPMI) update : 2025/08/29 |
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| Comment | Stopped distribution temporarily. Hybridoma producing a monoclonal antibody against mouse B7-H1. Possible to culture in RPMI1640 medium. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) The RECIOIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent from the DEPOCITOR. 2) The RECIPIENT shall be a non-profit entity only. 3) The BIOLOGICAL RESOURCE shall be used for teaching or not-for-profit research purposes only. 4) In publishing the research results obtained by the use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR’s SCIENTIST is requested. Also the RECIPIENT agree to acknowledgment to the DEPOSITOR’s SCIENTIST as a coauthor in such publication that the BIOLOGICAL RESOURCE materially contributes to the publications. 5) if the RECIPIENT discovers creates or derives new material or invention though the use of the BIOLOGICAL RESOURCE, the ownership of any patent or other intellectual property right to the material or invention shall be negotiated between PROVIDER and RECIPIENT | |||
| Remarks | ||||
| approver's address |
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| Order Form | Order Form(C-0005.pdf) Approval Form(C-0006.pdf) MTA(C-0007.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Azuma, Miyuki | ||
| Originator | Azuma, Miyuki | |||
| Year of deposit | 2006 | |||
| Animal | _mouse x rat < Hybrid | |||
| Tissue | hybridoma | |||
| Classification | hybrid cells | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_C664 | hybridoma | Fusion_cell_1 name | spleen |
| Fusion_cell_1 strain | rat, SD | |||
| Fusion_cell_1 gender | Female | |||
| Fusion_cell_2 name | P3U1 | |||
| Fusion_cell_2 strain | mouse | Fusion method | PEG, HAT selection | Antibody name | MIH5 |
| Subclass | IgG2a | |||
| Immunogen | mouse B7-H1 transfected L5178Y | Antigen_name | B7-H1/PD-L1 | |
| Antigen_character | mouse B7-H1 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture method | General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese) | |||
| Culture medium | RPMI1640 + 10% FBS | |||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage ratio | 1 : 10 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Antibody subclass | IgG2a | |||
| Images | ||||
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| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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