Cell No. : Cell Name RCB2309 : JFP-J1  update : 2024/01/17
Comment			Hybridoma producing monoclonal antibody against GFP. Rat IgG2a monoclonal antibody.
Comment from the depositor
Terms and conditions			1) Material is considered the proprietary property of Mitsubishi Kagaku Institute of Life Science (MITILA) and Mitsubishi Chemical Corporation (MCC) . 2) Recipient Investigator agrees not to use Material for profit making or commercial purposes. 3) Recipient Investigator shall acknowledge the source of Material in any publication containing experimental results obtained with the use of Material. 4) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Neurochem 1999;19:3918-3925) designated by the DEPOSITOR is required. 5) At the conclusion of the research for which Material was requested, Recipient shall dispose of unused Material in an appropriate legal manner. 6) After providing Material, RIKEN BRC shall inform MCC of followings;names of Recipient and Recipient Investigator, purpose and date.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Takayama, Hajime
		Originator	Fujita, Shinobu
		Year of deposit	2006
		Another name	851D7
		Animal	_mouse x rat < Hybrid
		Tissue	hybridoma
		Classification	hybrid cells
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_C478
	hybridoma	Fusion_cell_1 name	P3-X63-Ag8.653
		Fusion_cell_1 strain	mouse
		Fusion_cell_2 name	spleen cell
		Fusion_cell_2 strain	rat, Wistar
		Fusion_cell_2 gender	Female
		Fusion method	PEG
		Antibody name	JFP-J1
		Subclass	IgG2a
		Immunogen	recombinant GFP
		Antigen_name	GFP
		Antigen_m.w.	30kDa
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Antibody subclass		IgG2a
Images			deposit info	lot info
Reference information		Reference	1
		User's Publication	3

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Reference

3615      J Neurochem  1999  19:3918-392

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User's Publication

7078  Hasegawa K, Yasuda T, Shiraishi C, Fujiwara K, Przedborski S, Mochizuki H, Yoshikawa K.  Promotion of mitochondrial biogenesis by necdin protects neurons against mitochondrial insults.  Nat Commun  2016  7:10943  PubMed ID: 26971449   DOI: 10.1038/ncomms10943

14312  Nagai T, Furusho Y, Li H, Hasui K, Matsukita S, Sueyoshi K, Yanagi M, Hatae M, Takao S, Matsuyama T.  Production of a High-affinity Monoclonal Antibody Reactive with Folate Receptors Alpha and Beta  Monoclon Antib Immunodiagn Immunother  2015  34(3):181-90  PubMed ID: 26090596   DOI: 10.1089/mab.2014.0072

18237  Mazzolini R, Rodrigues P, Bazzocco S, Dopeso H, Ferreira AM, Mateo-Lozano S, Andretta E, Woerner SM, Alazzouzi H, Landolfi S, Hernandez-Losa J, Macaya I, Suzuki H, Ramón y Cajal S, Mooseker MS, Mariadason JM, Gebert J, Hofstra RM, Reventós J, Yamamoto H, Schwartz S Jr, Arango D.  Brush border myosin Ia inactivation in gastric but not endometrial tumors  Int J Cancer  2013  132(8):1790-9  PubMed ID: 23002058   DOI: 10.1002/ijc.27856