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RCB2280 : HCE-T  update : 2019/01/09
CommentSV40-Adeno vector transformed cornea cells. 64kD keratin producing. Choleratoxin-free medium.
Comment from the depositor
Restriction (Category)f
Terms and conditions1) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Invest Ophthalmol Vis Sci. 1995;36:614-621)
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Araki-Sasaki Kaoru
Originator Araki-Sasaki Kaoru
Year of deposit 2005
Original cell RCB1384
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 49 years
Tissue cornea
Case history maxillary sinus carcinoma
Classification transformed
Recombinant recombinant
Exogene SV40-large T
Memo_1 Mycoplasma was eliminated with MC-210 by the depositor.
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM/HamF12 + 5% FBS + 5μg/ml Insulin + 10ng/ml human EGF + 0.5% DMSO
Antibiotics Free
Passage method 0.25% trypsin or (0.25% trypsin + 0.02% EDTA)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 33


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Reference
2263  Araki-Sasaki K, Ohashi Y, Sasabe T, Hayashi K, Watanabe H, Tano Y, Handa H  An SV40-immortalized human corneal epithelial cell line and its characterization.  Invest Ophthalmol Vis Sci  1995  36:614-21 
2262  Zhang Y, Araki-Sasaki K, Handa H, Akhtar, R A  Effect of carbachol on phospholipase C-mediated phosphatidylinositol 4,5-bisphosphate hydrolysis, and its modulation by isoproterenol in rabbit corneal epithelial cells.  Curr Eye Res  1995  14:563-71 
2261  Araki K, Ohashi Y, Sasabe T, Kinoshita S, Hayashi K, Yang, X Z, Hosaka Y, Aizawa S, Handa H  Immortalization of rabbit corneal epithelial cells by a recombinant SV40-adenovirus vector.  Invest Ophthalmol Vis Sci  1993  34:2665-71 

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User's Publication
7260  Wu MF, Stachon T, Wang J, Song X, Colanesi S, Seitz B, Wagenpfeil S, Langenbucher A, Szentmary N.  Effect of Keratocyte Supernatant on Epithelial Cell Migration and Proliferation After Corneal Crosslinking (CXL).  Curr. Eye Res.  2015  41:466-73  PubMed ID: 26236938
7396  Hafner A, Lovri? J, Romi? MD, Jureti? M, Pepi? I, Cetina-?i?mek B, Filipovi?-Gr?i? J.  Evaluation of cationic nanosystems with melatonin using an eye-related bioavailability prediction model.  Eur J Pharm Sci  2015  75:142-50  PubMed ID: 25869457
7500  Verstraelen J, Reichl S.  Upregulation of P-glycoprotein expression by ophthalmic drugs in different corneal in-vitro models.  J. Pharm. Pharmacol.  2015  67:605-15  PubMed ID: 25643948
7623  Sharma S, Mohanty S, Gupta D, Jassal M, Agrawal AK, Tandon R.  Cellular response of limbal epithelial cells on electrospun poly-ε-caprolactone nanofibrous scaffolds for ocular surface bioengineering: a preliminary in vitro study.  Mol. Vis.  2011  17:2898-910  PubMed ID: 22128237
7815  Kato M, Hagiwara Y, Oda T, Imamura-Takai M, Aono H, Nakamura M.  Beneficial pharmacological effects of selective glucocorticoid receptor agonist in external eye diseases.  J Ocul Pharmacol Ther  2011  27:353-60  PubMed ID: 21574866
7974  Youn HY, McCanna DJ, Sivak JG, Jones LW.  In vitro ultraviolet-induced damage in human corneal, lens, and retinal pigment epithelial cells.  Mol. Vis.  2011  17:237-46  PubMed ID: 21270970
9715  Esaki Y, Shimazaki A, Pellinen P.  Ocular Tolerability of Preservative-Free Tafluprost and Latanoprost: in vitro and in vivo Comparative Study.  Open Ophthalmol J  2016  10:146-53  PubMed ID: 27347250
9744  Hakkarainen JJ, Reinisalo M, Ragauskas S, Seppänen A, Kaja S, Kalesnykas G.  Acute cytotoxic effects of marketed ophthalmic formulations on human corneal epithelial cells.  Int J Pharm  2016  511:73-78  PubMed ID: 27374205
9749  Fuwa M, Ueda K, Akaishi T, Yamashita N, Kirihara T, Shimazaki A, Mano H, Kawazu K.  Advantages of Efficacy and Safety of Fixed-Dose Tafluprost/Timolol Combination Over Fixed-Dose Latanoprost/Timolol Combination.  PLoS ONE  2016  11:e0158797  PubMed ID: 27383260
9755  Wu MF, Stachon T, Langenbucher A, Seitz B, Szentmáry N.  Effect of Amniotic Membrane Suspension (AMS) and Amniotic Membrane Homogenate (AMH) on Human Corneal Epithelial Cell Viability, Migration and Proliferation In Vitro.  Curr. Eye Res.  2017  42:351-357  PubMed ID: 27420558
9778  Koyama S, Narita E, Shimizu Y, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.  Int J Environ Res Public Health  2016  13:  PubMed ID: 27509516
9779  Koyama S, Narita E, Shimizu Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.  Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.  Int J Environ Res Public Health  2016  13:  PubMed ID: 27527204
9821  Kim HJ, Kim JY, Lee BH, Choi SH, Rhim H, Kim HC, Ahn SY, Jeong SW, Jang M, Cho IH, Nah SY.  Gintonin, an exogenous ginseng-derived LPA receptor ligand, promotes corneal wound healing.  J. Vet. Sci.  2017  18:387-397  PubMed ID: 27586470
9965  Utsunomiya T, Ishibazawa A, Nagaoka T, Hanada K, Yokota H, Ishii N, Yoshida A.  Transforming Growth Factor-β Signaling Cascade Induced by Mechanical Stimulation of Fluid Shear Stress in Cultured Corneal Epithelial Cells.  Invest. Ophthalmol. Vis. Sci.  2016  57:6382-6388  PubMed ID: 27898984
9974  Santander-Borrego M, Taran E, Shadforth AM, Whittaker AK, Chirila TV, Blakey I.  Hydrogels with Lotus Leaf Topography: Investigating Surface Properties and Cell Adhesion.  Langmuir  2017  33:485-493  PubMed ID: 28054787
10034  Jamil B, Abbasi R, Abbasi S, Imran M, Khan SU, Ihsan A, Javed S, Bokhari H, Imran M.  Encapsulation of Cardamom Essential Oil in Chitosan Nano-composites: In-vitro Efficacy on Antibiotic-Resistant Bacterial Pathogens and Cytotoxicity Studies.  Front Microbiol  2016  7:1580  PubMed ID: 27757108
10189  Juretić M, Jurišić Dukovski B, Krtalić I, Reichl S, Cetina-Čižmek B, Filipović-Grčić J, Lovrić J, Pepić I.  HCE-T cell-based permeability model: A well-maintained or a highly variable barrier phenotype?  Eur J Pharm Sci  2017  104:23-30  PubMed ID: 28315467
10251  Nishida T, Kuse Y, Mochizuki K, Shimazawa M, Yamamoto T, Hara H.  Protective effects of fluoroquinolones on UV-induced damage of cultured ocular cell lines.  Eur. J. Pharmacol.  2017  806:59-66  PubMed ID: 28395990
10412  Hou A, Tin MQ, Tong L.  Toll-like receptor 2-mediated NF-kappa B pathway activation in ocular surface epithelial cells.  Eye Vis (Lond)  2017  4:17  PubMed ID: 28706958
10627  Beißner N, Zorn-Kruppa M, Reichl S.  Parameter study of shipping conditions for the ready-to-use application of a 3D human hemicornea construct in drug absorption studies.  Int J Pharm  2018  536:377-387  PubMed ID: 29191484
10824  Juretić M, Cetina-Čižmek B, Filipović-Grčić J, Hafner A, Lovrić J, Pepić I.  Biopharmaceutical evaluation of surface active ophthalmic excipients using in vitro and ex vivo corneal models.  Eur J Pharm Sci  2018  120:133-141  PubMed ID: 29702232
10983  Meng Z, Klinngam W, Edman MC, Hamm-Alvarez SF.  Interferon-γ treatment in vitro elicits some of the changes in cathepsin S and antigen presentation characteristic of lacrimal glands and corneas from the NOD mouse model of Sjo¨gren’s Syndrome  PLoS One  2017  12:e0184781  PubMed ID: 28902875
11118  Yin Y, Zong R, Bao X, Zheng X, Cui H, Liu Z, Zhou Y.  Oxidative Stress Suppresses Cellular Autophagy in Corneal Epithelium.  Invest. Ophthalmol. Vis. Sci.  2018    PubMed ID: 29971448
11136  Gillies PJ, Richardson NA, Walshe J, Stephenson SA, Dawson RA, Harkin DG.  Demonstration of P-selectin expression and potential function in human corneal epithelial cells.  Exp. Eye Res.  2018    PubMed ID: 30025918
11142  Marek V, Melik-Parsadaniantz S, Villette T, Montoya F, Baudouin C, Brignole-Baudouin F, Denoyer A.  Blue light phototoxicity toward human corneal and conjunctival epithelial cells in basal and hyperosmolar conditions.  Free Radic. Biol. Med.  2018    PubMed ID: 30040995
11175  Png E, Hou A, Tong L.  Mechanistic role of transglutaminase-2 in focal adhesions.  Sci Rep  2018    PubMed ID: 30120307
11291  von Deylen D, Dreher C, Seidelmann O, Reichl S.  New Classes of Polycationic Compounds as Preservatives for Ophthalmic Formulations.  Pharm. Res.  2018    PubMed ID: 30411196
11370  Xu P, Kai C, Kawasaki S, Kobayashi Y, Yamamoto K, Tsujikawa M, Hayashi R, Nishida K.  A New in Vitro Model of GDLD by Knocking Out TACSTD2 and Its Paralogous Gene EpCAM in Human Corneal Epithelial Cells.  Transl Vis Sci Technol  2018    PubMed ID: 30619650
4084  Akiko TOJU, Yuko UDAKA, Mayumi TSUJI, Hideto OYAMADA, Junichiro KIZAKI, Eiji TOMOYORI, Yuji KIUCHI  Suppressive Effects of Catechins in UV-Induced Cytotoxicity of Human Corneal Epithelial Cells  Showa Univ J Med Sci  2018  3, 73~85 
4191  Krtalić I, Radošević S, Hafner A, Grassi M, Nenadić M, Cetina-Čižmek B, Filipović-Grčić J, Pepić I, Lovrić J.  D-Optimal Design in the Development of Rheologically Improved In Situ Forming Ophthalmic Gel.  J Pharm Sci  2018    PubMed ID: 29421217
4192  Beiβner N, Mattern K, Dietzel A, Reichl S.  DynaMiTES - A dynamic cell culture platform for in vitro drug testing PART 2 - Ocular DynaMiTES for drug absorption studies of the anterior eye.  Eur J Pharm Biopharm  2018    PubMed ID: 28377274
4193  Klinngam W, Fu R, Janga SR, Edman MC, Hamm-Alvarez SF.  Cathepsin S Alters the Expression of Pro-Inflammatory Cytokines and MMP-9, Partially through Protease-Activated Receptor-2, in Human Corneal Epithelial Cells.  Int J Mol Sci  2018    PubMed ID: 30423938
4477  Tan G, Li J, Yang Q, Wu A, Qu DY, Wang Y, Ye L, Bao J, Shao Y.  Air pollutant particulate matter 2.5 induces dry eye syndrome in mice.  Sci Rep  2018  8(1):17828  PubMed ID: 30546125



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