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RCB2239 : HE19  update : 2018/07/06
CommentHuman fibroblast-like cells derived from embryo. Not immortalized.
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Watanabe, Masami
Originator Watanabe, Masami
Year of deposit 2002
Animal human < Mammals
Genus Homo
Species sapiens
Tissue embryo
Classification normal
Year of origin 1988
Lifespan finite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS + 1mM Sodium Pyruvate + 0.2mM Serine + 0.2mM Aspartate
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
7593  Upadhyay AK, Rotili D, Han JW, Hu R, Chang Y, Labella D, Zhang X, Yoon YS, Mai A, Cheng X.  An analog of BIX-01294 selectively inhibits a family of histone H3 lysine 9 Jumonji demethylases.  J. Mol. Biol.  2012  416:319-27  PubMed ID: 22227394



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