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RCB2150 : EKOP  update : 2018/07/06
CommentSubline of KOP cell line, expressing FLAG-tagged EXIP. Zeocin-resistant.
Comment from the depositor
Terms and conditions1) Research purpose only. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2005;337:415-421) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sudo, Tatsuhiko
Originator Sudo, Tatsuhiko
Year of deposit 2005
Animal mouse < Mammals
Gender Female
Tissue embryo
Classification mutant
Recombinant recombinant
Exogene human EXIP, neo, zeo
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 0


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Reference
3454  Sudo, Tatsuhiko, Kawai, Kayoko, Matsuzaki, Hiroshi, Osada, Hiroyuki  p38 mitogen-activated protein kinase plays a key role in regulating MAPKAPK2 expression.  Biochem Biophys Res Commun  2005  337:415-21  PubMed ID: 16198317

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