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RCB2148 : KOP  update : 2018/07/06
CommentMouse fibroblast-like cell line lacking p38 MAP kinase expression.
Comment from the depositor
Restriction (Category)f
Terms and conditions1) Research purpose only. 2) In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (Biochem Biophys Res Commun 2005;337:415-421) designated by the DEPOSITOR is required.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Sudo, Tatsuhiko
Originator Sudo, Tatsuhiko
Year of deposit 2005
Animal mouse < Mammals
Gender Female
Tissue embryo
Classification mutant
Recombinant recombinant
Exogene knockout of p38, neo
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 1


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Reference
Sudo, Tatsuhiko, Kawai, Kayoko, Matsuzaki, Hiroshi, Osada, Hiroyuki    Biochem Biophys Res Commun  2005  337:415-21 

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User's Publication
Kitamura A, Maekawa Y, Uehara H, Izumi K, Kawachi I, Nishizawa M, Toyoshima Y, Takahashi H, Standley DM, Tanaka K, Hamazaki J, Murata S, Obara K, Toyoshima I, Yasutomo K.  A mutation in the immunoproteasome subunit PSMB8 causes autoinflammation and lipodystrophy in humans.  J. Clin. Invest.  2011  121:4150-60  PubMed ID: 21881205



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