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RCB2145 : PC-3  update : 2018/08/02
CommentHuman cell line derived from bone metastasis of prostatic cancer. TKG0600 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2005
Original cell TKG0600
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 62 years
Tissue prostate, bone meta
Case history prostatic carcinom
Metastastatic ability Yes
Metastatsd tissue bone
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0600)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 23

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5121  Kirk D, Szalay MF, Kaighn ME.  Modulation of growth of a human prostatic cancer cell line (PC-3) in agar culture by normal human lung fibroblasts.  Cancer Res  1981  41(3):1100-3  PubMed ID: 7459854
5120  Ohnuki Y, Marnell MM, Babcock MS, Lechner JF, Kaighn ME.  Chromosomal analysis of human prostatic adenocarcinoma cell lines.  Cancer Res  1980  40(3):524-34  PubMed ID: 7471073
5118  Kaighn ME, Narayan KS, Ohnuki Y, Lechner JF, Jones LW.  Establishment and characterization of a human prostatic carcinoma cell line (PC-3).  Invest Urol  1979  17(1):16-23  PubMed ID: 447482
5119  Kaighn ME, Lechner JF, Narayan KS, Jones LW.  Prostate carcinoma: tissue culture cell lines.  Natl Cancer Inst Monogr  1978  (49):17-21  PubMed ID: 571045

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User's Publication
9833  Keiichi Oono, Kazuo Ohtake, Chie Watanabe, Sachiko Shiba, Takashi Sekiya, Keizo Kasono  Contribution of Pyk2 Pathway and Reactive Oxygen Species (ROS) to the Anti-Cancer Effects of Eicosapentaenoic Acid (EPA) in PC3 Prostate Cancer Cells  Lipids Health Dis  2020  19(1):15  PubMed ID: 32005121
11271  Itoh A, Nonaka Y, Ogawa T, Nakamura T, Nishi N.  Galectin-9 induces atypical ubiquitination leading to cell death in PC-3 prostate cancer cells.  Glycobiology  2019    PubMed ID: 30383222
11464  Ishigami-Yuasa M, Ekimoto H, Kagechika H.  Class IIb HDAC Inhibition Enhances the Inhibitory Effect of Am80, a Synthetic Retinoid, in Prostate Cancer.  Biol. Pharm. Bull.  2019    PubMed ID: 30828077
11507  Murata K, Saga R, Monzen S, Tsuruga E, Hasegawa K, Hosokawa Y.  Understanding the mechanism underlying the acquisition of radioresistance in human prostate cancer cells.  Oncol Lett  2019    PubMed ID: 31186811
11639  Taniguchi K, Ii H, Kageyama S, Takagi H, Chano T, Kawauchi A, Nakata S.  Depletion of gamma-glutamylcyclotransferase inhibits cancer cell growth by activating the AMPK-FOXO3a-p21 axis.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31345573
11693  Kensaku Hayashi, Momoko Kumemura, Shohei Kaneda, Vivek Menon, Laurent Jalabert, Saeko Tachikawa, Mehmet C. Tarhan, Teruo Fujii, Beomjoon Kim, Hiroyuki Fujita  Optimization of Surface Treatment on MEMS Probes for Single-cell Capture and Release  Sensors and Materials  2019   
11800  Koizumi A, Narita S, Nakanishi H, Ishikawa M, Eguchi S, Kimura H, Takasuga S, Huang M, Inoue T, Sasaki J, Yoshioka T, Habuchi T, Sasaki T.  Increased fatty acyl saturation of phosphatidylinositol phosphates in prostate cancer progression.  Sci Rep  2019    PubMed ID: 31520002
11807  Takagi H, Ii H, Kageyama S, Hanada E, Taniguchi K, Yoshiya T, Chano T, Kawauchi A, Nakata S.  Blockade of γ-Glutamylcyclotransferase Enhances Docetaxel Growth Inhibition of Prostate Cancer Cells.  Anticancer Res.  2019    PubMed ID: 31519583
10845  Taniguchi K, Matsumura K, Ii H, Kageyama S, Ashihara E, Chano T, Kawauchi A, Yoshiki T, Nakata S.  Depletion of gamma-glutamylcyclotransferase in cancer cells induces autophagy followed by cellular senescence.  Am J Cancer Res  2018  8:650-661  PubMed ID: 29736310
11105  Senga S, Kobayashi N, Kawaguchi K, Ando A, Fujii H.  Fatty acid-binding protein 5 (FABP5) promotes lipolysis of lipid droplets, de novo fatty acid (FA) synthesis and activation of nuclear factor-kappa B (NF-κB) signaling in cancer cells.  Biochim Biophys Acta Mol Cell Biol Lipids  2018  1863(9):1057-1067  PubMed ID: 29906613
11160  Languon S, Tuffour I, Quayson EE, Appiah-Opong R, Quaye O.  In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana.  J Evid Based Integr Med  2018    PubMed ID: 30088418
4319  Liu Q, Wu Y, Seino H, Haga T, Yoshizawa T, Morohashi S, Kijima H.  Correlation between DEC1/DEC2 and epithelial‑mesenchymal transition in human prostate cancer PC‑3 cells.  Mol Med Rep  2018    PubMed ID: 30106153
10506  Doi H, Kida T, Nishino K, Nakatsuji M, Sakamoto S, Shimizu S, Teraoka Y, Tamura Y, Kataoka Y, Inui T.  Solubility-Improved 10-O-Substituted SN-38 Derivatives with Antitumor Activity.  ChemMedChem  2017  12:1715-1722  PubMed ID: 28891271
10973    Dynamics of Morphological Changes of Adherent Cultured Cells at a Range of Low Temperatures near Physiological Temperature (Time-Lapse Imaging by Digital Holographic Microscopy)  Cryobiology and Cryotechnology  2017  63, 45~51 
4534  Cui C, Hanyu M, Hatori A, Zhang Y, Xie L, Ohya T, Fukada M, Suzuki H, Nagatsu K, Jiang C, Luo R, Shao G, Zhang M, Wang F.  Synthesis and evaluation of [64Cu]PSMA-617 targeted for prostate-specific membrane antigen in prostate cancer.  Am J Nucl Med Mol Imaging  2017  7(2):40-52  PubMed ID: 28533936
7050  Kajiura K, Masuda K, Naruto T, Kohmoto T, Watabnabe M, Tsuboi M, Takizawa H, Kondo K, Tangoku A, Imoto I.  Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.  Oncotarget  2016  8:2890-2905  PubMed ID: 27926516
9615  Ohya S, Kanatsuka S, Hatano N, Kito H, Matsui A, Fujimoto M, Matsuba S, Niwa S, Zhan P, Suzuki T, Muraki K.  Downregulation of the Ca(2+)-activated K(+) channel KC a3.1 by histone deacetylase inhibition in human breast cancer cells.  Pharmacol Res Perspect  2016  4:e00228  PubMed ID: 27069638
9862  Sakura Y, Tsuboi K, Uyama T, Zhang X, Taoka R, Sugimoto M, Kakehi Y, Ueda N.  A quantitative study on splice variants of N-acylethanolamine acid amidase in human prostate cancer cells and other cells.  Biochim. Biophys. Acta  2016  1861:1951-1958  PubMed ID: 27693242
10024  Matsumura K, Nakata S, Taniguchi K, Ii H, Ashihara E, Kageyama S, Kawauchi A, Yoshiki T.  Depletion of γ-glutamylcyclotransferase inhibits breast cancer cell growth via cellular senescence induction mediated by CDK inhibitor upregulation.  BMC Cancer  2016  16:748  PubMed ID: 27658708
10908    Extracts of Codiaeum variegatum (L.) A. Juss is Cytotoxic on Human Leukemic, Breast and Prostate Cancer Cell Lines  Journal of Applied Pharmaceutical Science  2016  6, 087-093 
6034  Liu Q, Wu Y, Yoshizawa T, Yan X, Morohashi S, Seino H, Kato Y, Kijima H.  Basic helix-loop-helix transcription factor DEC2 functions as an anti-apoptotic factor during paclitaxel-induced apoptosis in human prostate cancer cells.  Int J Mol Med  2016  38(6):1727-1733  PubMed ID: 27840924
7447  Sakane C, Ohta H, Shidoji Y.  Measurement of lysine-specific demethylase-1 activity in the nuclear extracts by flow-injection based time-of-flight mass spectrometry.  J Clin Biochem Nutr  2015  56:123-31  PubMed ID: 25759518
5547  Orui H, Imaizumi S, Ogino T, Motoyama T.  Effects of bone morphogenetic protein-2 on human tumor cell growth and differentiation: a preliminary report.  J Orthop Sci  2000  5(6):600-4  PubMed ID: 11180925

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