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RCB2138 : KLM-1  update : 2018/08/02
CommentHuman cell line derived from pancreatic cancer. RCB1972 was derived from the same patient. TKG0490 (Deposited from Tohoku Univ.).
Comment from the depositor
Use conditiona
Use condition descriptionBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Kobari, M.
Year of deposit 2005
Original cell TKG0490
Animal human < Mammals
Genus Homo
Species sapiens
Tissue pancreas
Case history pancreatic carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0490)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 7


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Reference

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User's Publication
Calle AS, Nair N, Oo AK, Prieto-Vila M, Koga M, Khayrani AC, Hussein M, Hurley L, Vaidyanath A, Seno A, Iwasaki Y, Calle M, Kasai T, Seno M.  A new PDAC mouse model originated from iPSCs-converted pancreatic cancer stem cells (CSCcm).  Am J Cancer Res  2017  6:2799-2815  PubMed ID: 28042501
Namba T, Kodama R, Moritomo S, Hoshino T, Mizushima T.  Zidovudine, an anti-viral drug, resensitizes gemcitabine-resistant pancreatic cancer cells to gemcitabine by inhibition of the Akt-GSK3β-Snail pathway.  Cell Death Dis  2015  6:e1795  PubMed ID: 26111057
Hollevoet K, Mason-Osann E, Muller F, Pastan I.  Methylation-associated partial down-regulation of mesothelin causes resistance to anti-mesothelin immunotoxins in a pancreatic cancer cell line.  PLoS ONE  2015  10:e0122462  PubMed ID: 25803818
Tarhan YE, Kato T, Jang M, Haga Y, Ueda K, Nakamura Y, Park JH.  Morphological Changes, Cadherin Switching, and Growth Suppression in Pancreatic Cancer by GALNT6 Knockdown.  Neoplasia  2016  18:265-272  PubMed ID: 27237318
Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T.  Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78.  Biochem. Biophys. Res. Commun.  2017  492:33-40  PubMed ID: 28811106



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