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Cell No. : Cell Name
RCB2136 : YST-1  update : 2018/08/02
CommentHuman cell line derived from schwannoma. TKG0454 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
Tokyo women's medical university hospital
Depatment of Surgery Pathology
8-1,Kawada-cho,Sinjuku,Tokyo 162-8666 Japan
Dr.Nagashima, Yoji
Fax. +81-3-5269-7358
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Nagashima, Yoji
Year of deposit 2005
Original cell TKG0454
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 8 years
Tissue right arm
Case history malignant schwannoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0454)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting or 0.25% trypsin (<1min.)
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 1


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Reference

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User's Publication
8364  Wakao S, Hayashi T, Kitada M, Kohama M, Matsue D, Teramoto N, Ose T, Itokazu Y, Koshino K, Watabe H, Iida H, Takamoto T, Tabata Y, Dezawa M.  Long-term observation of auto-cell transplantation in non-human primate reveals safety and efficiency of bone marrow stromal cell-derived Schwann cells in peripheral nerve regeneration.  Exp. Neurol.  2010  223:537-47  PubMed ID: 20153320



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