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RCB2127 : COLO205  update : 2018/08/02
CommentHuman cell line derived from colon cancer. Derived from ascites metastasis. Cell growth is slow. TKG0457 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2005
Original cell TKG0457
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 70 years
Tissue colon, ascites meta
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0457)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
5100  Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model.  Semple TU, Quinn LA, Woods LK, Moore GE.  Cancer Res  1978  38(5):1345-55  PubMed ID: 565251

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User's Publication
10185  Comprehensive molecular exploration identified promoter DNA methylation of the CRBP1 gene as a determinant of radiation sensitivity in rectal cancer.  Yokoi K, Yamashita K, Ishii S, Tanaka T, Nishizawa N, Tsutsui A, Miura H, Katoh H, Yamanashi T, Naito M, Sato T, Nakamura T, Watanabe M.  Br. J. Cancer  2017  116:1046-1056  PubMed ID: 28291773
10498  Histopathological and genotypic characterization of metastatic colorectal carcinoma with PD-L1 (CD274)-expression: Possible roles of tumour micro environmental factors for CD274 expression.  Inaguma S, Lasota J, Felisiak-Golabek A, Kowalik A, Wang Z, Zieba S, Kalisz J, Ikeda H, Miettinen M.  J Pathol Clin Res  2017  3:268-278  PubMed ID: 29085667
10556  Rebamipide suppresses 5-fluorouracil-induced cell death via the activation of Akt/mTOR pathway and regulates the expression of Bcl-2 family proteins.  Tsubaki M, Takeda T, Asano RT, Matsuda T, Fujimoto SI, Itoh T, Imano M, Satou T, Nishida S.  Toxicol In Vitro  2018  46:284-293  PubMed ID: 29054700
10702  Mechanism of recipient cell-dependent differences in exosome uptake.  Horibe S, Tanahashi T, Kawauchi S, Murakami Y, Rikitake Y.  BMC Cancer  2018  18:47  PubMed ID: 29306323
6410  Is parainfluenza virus a threatening virus for human cancer cell lines?  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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