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RCB2094 : MIA Paca2  update : 2018/08/02
CommentHuman cell line derived from pancreatic cancer. TKG0227 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Yunis, A
Year of deposit 2005
Original cell TKG0227
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 65 years
Tissue pancreas
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0227)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 14
User's Publication 31


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Reference
3460  Yunis, A A, Arimura, G K, Russin, D J  Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.  Int J Cancer  1977  19:128-35  PubMed ID: 832918
3301  Yamada T, Okajima F, Ohwada S, Kondo Y  Growth inhibition of human pancreatic cancer cells by sphingosylphosphorylcholine and influence of culture conditions.  Cell Mol Life Sci  1997  53:435-41  PubMed ID: 9176562
5076  Wu M, Arimura GK, Yunis AA.  Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells.  Biochemistry  1977  16(9):1908-13  PubMed ID: 15590
5077  Wu MC, Arimura GK, Yunis AA.  Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase.  Int J Cancer  1978  22(6):728-33  PubMed ID: 363626
5078  Wu M, Cini JK, Yunis AA.  Purification of a colony-stimulating factor from cultured pancreatic carcinoma cells.  J Biol Chem  1979  254(14):6226-8  PubMed ID: 312800
5079  Allen L, Meck R, Yunis A.  The inhibition of gamma-glutamyl transpeptidase from human pancreatic carcinoma cells by (alpha S,5S)-alpha-amino-3-chloro-4,5-dihydro-5-isoxazoleacetic acid (AT-125; NSC-163501).  Res Commun Chem Pathol Pharmacol  1980  27(1):175-82  PubMed ID: 6102405
5080  Wu MC, Arimura GK, Holcenberg JS, Yunis AA.  Sensitivity of cultured pancreatic carcinoma cells to Acinetobacter glutaminase-asparaginase.  In Vitro  1982  18(9):750-4  PubMed ID: 7173949
5081  Fountzilas G, Gratzner H, Lim LO, Yunis AA.  Sensitivity of cultured human pancreatic carcinoma cells to dihydroxyanthracenedione.  Int J Cancer  1984  ;33(3):347-53  PubMed ID: 6698638
5082  Fountzilas G, Lim LO, Yunis AA.  Sensitivity of human pancreatic carcinoma cell line (MIA PaCa-2) to Bisantrene and Theprubicin in vitro.  In Vitro  1984  20(9):685-91  PubMed ID: 6500607
5083  Yunis AA, Jimenez JJ, Wu MC, Andreotti PE.  Further evidence supporting an in vivo role for colony-stimulating factor.  Exp Hematol  1984  12(11):838-43  PubMed ID: 6334613
5084  Fountzilas G, Gratzner H, Lim LO, Yunis AA.  Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2).  J Natl Cancer Inst  1986  76(1):37-43  PubMed ID: 3455740
5085  Ziboh VA, Wong T, Wu MC, Yunis AA.  Modulation of colony stimulating factor-induced murine myeloid colony formation by S-peptido-lipoxygenase products.  Cancer Res  1986  46(2):600-3  PubMed ID: 3079666
5086  Shieh JH, Cini JK, Wu MC, Yunis AA.  Purification and characterization of human colony-stimulating factor 1 from human pancreatic carcinoma (MIA PaCa-2) cells.  Arch Biochem Biophys  1987  253(1):205-13  PubMed ID: 3545083
5087  Fountzilas G, Lim LO, Yunis AA.  The inhibitory effects of teniposide and homoharringtonine on the growth of pancreatic carcinoma cells in vitro.  Anticancer Res  1988  8(3):343-6  PubMed ID: 3389738

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User's Publication
7235  Tsukahara T, Haniu H, Matsuda Y.  The PTB-Associated Splicing Factor/Peroxisome Proliferator-Activated Receptor Gamma Axis Regulates Autophagosome Formation in Human Pancreatic Cancer Cells.  Biores Open Access  2015  4:319-25  PubMed ID: 26309807
7243  Yoshino Y, Ishioka C.  Inhibition of glycogen synthase kinase-3 beta induces apoptosis and mitotic catastrophe by disrupting centrosome regulation in cancer cells.  Sci Rep  2015  5:13249  PubMed ID: 26292722
7358  Akimoto M, Iizuka M, Kanematsu R, Yoshida M, Takenaga K.  Anticancer Effect of Ginger Extract against Pancreatic Cancer Cells Mainly through Reactive Oxygen Species-Mediated Autotic Cell Death.  PLoS ONE  2015  10:e0126605  PubMed ID: 25961833
7400  Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y.  Clinical significance of CD155 expression in human pancreatic cancer.  Anticancer Res.  2015  35:2287-97  PubMed ID: 25862891
7560  Shimodaira S, Hirabayashi K, Koya T, Higuchi Y, Sano K  Standard WT1 Expression Using Immunohistochemistry.  J Mol Biomark Diagn  2015  7:266 
7696  Seki T, Kokuryo T, Yokoyama Y, Suzuki H, Itatsu K, Nakagawa A, Mizutani T, Miyake T, Uno M, Yamauchi K, Nagino M.  Antitumor effects of α-bisabolol against pancreatic cancer.  Cancer Sci.  2011  102:2199-205  PubMed ID: 21883695
8011  Nishio R, Tsuchiya H, Yasui T, Matsuura S, Kanki K, Kurimasa A, Hisatome I, Shiota G.  Disrupted plasma membrane localization of equilibrative nucleoside transporter 2 in the chemoresistance of human pancreatic cells to gemcitabine (dFdCyd).  Cancer Sci.  2011  102:622-9  PubMed ID: 21205085
8294  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer.  World J. Gastroenterol.  2010  16:1854-8  PubMed ID: 20397262
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
10092  Doi T, Ishikawa T, Okayama T, Oka K, Mizushima K, Yasuda T, Sakamoto N, Katada K, Kamada K, Uchiyama K, Handa O, Takagi T, Naito Y, Itoh Y.  The JAK/STAT pathway is involved in the upregulation of PD-L1 expression in pancreatic cancer cell lines.  Oncol. Rep.  2017  37:1545-1554  PubMed ID: 28112370
10128  Koya T, Yanagisawa R, Higuchi Y, Sano K, Shimodaira S.  Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity.  Sci Rep  2017  7:42145  PubMed ID: 28191816
10228  Yamauchi A, Yamamura M, Katase N, Itadani M, Okada N, Kobiki K, Nakamura M, Yamaguchi Y, Kuribayashi F.  Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device.  BMC Cancer  2017  17:234  PubMed ID: 28359316
10399  Fujita M, Hasegawa A, Yamamori M, Okamura N.  In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancer.  J. Exp. Clin. Cancer Res.  2017  36:91  PubMed ID: 28673319
10537  Kawamura D, Takemoto Y, Nishimoto A, Ueno K, Hosoyama T, Shirasawa B, Tanaka T, Kugimiya N, Harada E, Hamano K.  Enhancement of cytotoxic effects of gemcitabine by Dclk1 inhibition through suppression of Chk1 phosphorylation in human pancreatic cancer cells.  Oncol. Rep.  2017  38:3238-3244  PubMed ID: 29048622
4453  Idichi T, Seki N, Kurahara H, Fukuhisa H, Toda H, Shimonosono M, Yamada Y, Arai T, Kita Y, Kijima Y, Mataki Y, Maemura K, Natsugoe S.  Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3p.  Oncotarget  2018    PubMed ID: 29988949
11242  Johzuka J, Ona T, Nomura M.  One Hour In Vivo-like Phenotypic Screening System for Anti-cancer Drugs Using a High Precision Surface Plasmon Resonance Device.  Anal Sci  2018    PubMed ID: 30305596
11358  Kibe S, Ohuchida K, Ando Y, Takesue S, Nakayama H, Abe T, Endo S, Koikawa K, Okumura T, Iwamoto C, Shindo K, Moriyama T, Nakata K, Miyasaka Y, Shimamoto M, Ohtsuka T, Mizumoto K, Oda Y, Nakamura M.  Cancer-associated acinar-to-ductal metaplasia within the invasive front of pancreatic cancer contributes to local invasion.  Cancer Lett.  2019    PubMed ID: 30590101
4298  Feng R, Morine Y, Ikemoto T, Imura S, Iwahashi S, Saito Y, Shimada M.  Nab-paclitaxel interrupts cancer-stromal interaction through C-X-C motif chemokine 10-mediated interleukin-6 downregulation in vitro.  Cancer Sci.  2018    PubMed ID: 29902349
5018  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Frizzled-2: A potential novel target for molecular pancreatic cancer therapy.  Oncol Lett  2014  7(1):74-78  PubMed ID: 24348824
5019  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  SU11274 suppresses proliferation and motility of pancreatic cancer cells.  Oncol Lett  2015  10(3):1468-1472, RM010391 , RM010392, RM010292, RM010394,   PubMed ID: 26622692
5020  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  nsulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media.  J Cell Biochem  2012  113(8):2714-20  PubMed ID: 22441995
5023  Jiang P, Watanabe H, Okada G, Ohtsubo K, Mouri H, Tsuchiyama T, Yao F, Sawabu N.  Diagnostic utility of aberrant methylation of tissue factor pathway inhibitor 2 in pure pancreatic juice for pancreatic carcinoma.  Cancer Sci  2006  97(11):1267-73  PubMed ID: 16965396
5025  Sakamoto K, Imai K, Higashi T, Taki K, Nakagawa S, Okabe H, Nitta H, Hayashi H, Chikamoto A, Ishiko T, Beppu T, Baba H.  Significance of P-cadherin overexpression and possible mechanism of its regulation in intrahepatic cholangiocarcinoma and pancreatic cancer.  Cancer Sci  2015  106(9):1153-62  PubMed ID: 26132727
5027  Nishizawa N1, Harada H1, Kumamoto Y1, Kaizu T, Katoh H, Tajima H, Ushiku H, Yokoi K, Igarashi K, Fujiyama Y, Okuwaki K, Iwai T, Watanabe M, Yamashita K1  Diagnostic potential of hypermethylation of the cysteine dioxygenase 1 gene (CDO1) promoter DNA in pancreatic cancer.  Cancer Sci  2019  110(9):2846-2855  PubMed ID: 31325200
5030  Tsukamoto M, Imai K, Ishimoto T, Komohara Y, Yamashita YI, Nakagawa S, Umezaki N, Yamao T, Kitano Y, Miyata T, Arima K, Okabe H, Baba Y, Chikamoto A, Ishiko T, Hirota M, Baba H.  PD-L1 expression enhancement by infiltrating macrophage-derived tumor necrosis factor-α leads to poor pancreatic cancer prognosis.  Cancer Sci  2019  110(1):310-320  PubMed ID: 30426611
5032  Igarashi S, Matsubara T, Harada K, Ikeda H, Sato Y, Sasaki M, Matsui O, Nakanuma Y.  Bile duct expression of pancreatic and duodenal homeobox 1 in perihilar cholangiocarcinogenesis.  Histopathology  2012  61(2):266-76  PubMed ID: 22594685
5430  Li C, Imai M, Yamasaki M, Hasegawa S, Takahashi N.  Effects of Pre- and Post-Administration of Vitamin A on the Growth of Refractory Cancers in Xenograft Mice.  Biol Pharm Bull  2017  40(4):486-494  PubMed ID: 28100867
5875  Mishra R, Miyamoto M, Yoshioka T, Ishikawa K, Matsumura Y, Shoji Y, Ichinokawa K, Itoh T, Shichinohe T, Hirano S, Kondo S.  Adenovirus-mediated eukaryotic initiation factor 4E binding protein-1 in combination with rapamycin inhibits tumor growth of pancreatic ductal adenocarcinoma in vivo.  Int J Oncol  2009  34(5):1231-40  PubMed ID: 19360336
6235  Li C, Imai M, Matsuura T, Hasegawa S, Yamasaki M, Takahashi N.  Inhibitory Effects of Retinol Are Greater than Retinoic Acid on the Growth and Adhesion of Human Refractory Cancer Cells.  Biol Pharm Bull  2016  39(4):636-40  PubMed ID: 2682241
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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