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RCB2094 : MIA Paca2  update : 2018/08/02
CommentHuman cell line derived from pancreatic cancer. TKG0227 (Deposited from Tohoku Univ.).
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Yunis, A
Year of deposit 2005
Original cell TKG0227
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 65 years
Tissue pancreas
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0227)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method pipetting
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 20


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Reference
3460  Yunis, A A, Arimura, G K, Russin, D J  Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase.  Int J Cancer  1977  19:128-35 

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User's Publication
7235  Tsukahara T, Haniu H, Matsuda Y.  The PTB-Associated Splicing Factor/Peroxisome Proliferator-Activated Receptor Gamma Axis Regulates Autophagosome Formation in Human Pancreatic Cancer Cells.  Biores Open Access  2015  4:319-25  PubMed ID: 26309807
7243  Yoshino Y, Ishioka C.  Inhibition of glycogen synthase kinase-3 beta induces apoptosis and mitotic catastrophe by disrupting centrosome regulation in cancer cells.  Sci Rep  2015  5:13249  PubMed ID: 26292722
7358  Akimoto M, Iizuka M, Kanematsu R, Yoshida M, Takenaga K.  Anticancer Effect of Ginger Extract against Pancreatic Cancer Cells Mainly through Reactive Oxygen Species-Mediated Autotic Cell Death.  PLoS ONE  2015  10:e0126605  PubMed ID: 25961833
7400  Nishiwada S, Sho M, Yasuda S, Shimada K, Yamato I, Akahori T, Kinoshita S, Nagai M, Konishi N, Nakajima Y.  Clinical significance of CD155 expression in human pancreatic cancer.  Anticancer Res.  2015  35:2287-97  PubMed ID: 25862891
7560  Shimodaira S, Hirabayashi K, Koya T, Higuchi Y, Sano K  Standard WT1 Expression Using Immunohistochemistry.  J Mol Biomark Diagn  2015  7:266 
7696  Seki T, Kokuryo T, Yokoyama Y, Suzuki H, Itatsu K, Nakagawa A, Mizutani T, Miyake T, Uno M, Yamauchi K, Nagino M.  Antitumor effects of α-bisabolol against pancreatic cancer.  Cancer Sci.  2011  102:2199-205  PubMed ID: 21883695
8011  Nishio R, Tsuchiya H, Yasui T, Matsuura S, Kanki K, Kurimasa A, Hisatome I, Shiota G.  Disrupted plasma membrane localization of equilibrative nucleoside transporter 2 in the chemoresistance of human pancreatic cells to gemcitabine (dFdCyd).  Cancer Sci.  2011  102:622-9  PubMed ID: 21205085
8294  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer.  World J. Gastroenterol.  2010  16:1854-8  PubMed ID: 20397262
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362
10092  Doi T, Ishikawa T, Okayama T, Oka K, Mizushima K, Yasuda T, Sakamoto N, Katada K, Kamada K, Uchiyama K, Handa O, Takagi T, Naito Y, Itoh Y.  The JAK/STAT pathway is involved in the upregulation of PD-L1 expression in pancreatic cancer cell lines.  Oncol. Rep.  2017  37:1545-1554  PubMed ID: 28112370
10128  Koya T, Yanagisawa R, Higuchi Y, Sano K, Shimodaira S.  Interferon-α-inducible Dendritic Cells Matured with OK-432 Exhibit TRAIL and Fas Ligand Pathway-mediated Killer Activity.  Sci Rep  2017  7:42145  PubMed ID: 28191816
10228  Yamauchi A, Yamamura M, Katase N, Itadani M, Okada N, Kobiki K, Nakamura M, Yamaguchi Y, Kuribayashi F.  Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device.  BMC Cancer  2017  17:234  PubMed ID: 28359316
10399  Fujita M, Hasegawa A, Yamamori M, Okamura N.  In vitro and in vivo cytotoxicity of troglitazone in pancreatic cancer.  J. Exp. Clin. Cancer Res.  2017  36:91  PubMed ID: 28673319
10537  Kawamura D, Takemoto Y, Nishimoto A, Ueno K, Hosoyama T, Shirasawa B, Tanaka T, Kugimiya N, Harada E, Hamano K.  Enhancement of cytotoxic effects of gemcitabine by Dclk1 inhibition through suppression of Chk1 phosphorylation in human pancreatic cancer cells.  Oncol. Rep.  2017  38:3238-3244  PubMed ID: 29048622
10850  Shigetaka Shimodaira, Koichi Hirabayashi, Terutsugu Koya, Yumiko Higuchi and Kenji Sano  Standard WT1 Expression Using Immunohistochemistry   J Mol Biomark Diagn  2015  7:1 
4453  Idichi T, Seki N, Kurahara H, Fukuhisa H, Toda H, Shimonosono M, Yamada Y, Arai T, Kita Y, Kijima Y, Mataki Y, Maemura K, Natsugoe S.  Involvement of anti-tumor miR-124-3p and its targets in the pathogenesis of pancreatic ductal adenocarcinoma: direct regulation of ITGA3 and ITGB1 by miR-124-3p.  Oncotarget  2018    PubMed ID: 29988949
11242  Johzuka J, Ona T, Nomura M.  One Hour In Vivo-like Phenotypic Screening System for Anti-cancer Drugs Using a High Precision Surface Plasmon Resonance Device.  Anal Sci  2018    PubMed ID: 30305596
11358  Kibe S, Ohuchida K, Ando Y, Takesue S, Nakayama H, Abe T, Endo S, Koikawa K, Okumura T, Iwamoto C, Shindo K, Moriyama T, Nakata K, Miyasaka Y, Shimamoto M, Ohtsuka T, Mizumoto K, Oda Y, Nakamura M.  Cancer-associated acinar-to-ductal metaplasia within the invasive front of pancreatic cancer contributes to local invasion.  Cancer Lett.  2019    PubMed ID: 30590101
4298  Feng R, Morine Y, Ikemoto T, Imura S, Iwahashi S, Saito Y, Shimada M.  Nab-paclitaxel interrupts cancer-stromal interaction through C-X-C motif chemokine 10-mediated interleukin-6 downregulation in vitro.  Cancer Sci.  2018    PubMed ID: 29902349



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