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Cell No. : Cell Name
RCB2081 : KUSA-A1  update : 2018/07/06
CommentMesenchymal stem cell line derived from C3H/He mouse strain.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Umezawa, Akihiro
Originator Umezawa, Akihiro
Year of deposit 2005
Animal mouse < Mammals
Strain name C3H/He
Gender Female
Tissue bone marrow
Classification normal
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives POWEREDBY10
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 2 x 10^(5) cells/100 mm dish
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 3


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Reference
2753  Umezawa A, Maruyama T, Segawa K, Shadduck RK, Waheed A, Hata J  Multipotent marrow stromal cell line is able to induce hematopoiesis in vivo.  J Cell Physiol  1992  151:197-205  PubMed ID: 1373147

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User's Publication
8577  Silvia Smaldone, Luca Carta, Francesco Ramirez  Establishment of Fibrillin-Deficient Osteoprogenitor Cell Lines Identifies Molecular Abnormalities Associated With Extracellular Matrix Perturbation of Osteogenic Differentiation  Cell Tissue Res  2011  344(3):511-7  PubMed ID: 21538048
8027  Toyoda M, Yamazaki-Inoue M, Itakura Y, Kuno A, Ogawa T, Yamada M, Akutsu H, Takahashi Y, Kanzaki S, Narimatsu H, Hirabayashi J, Umezawa A.  Lectin microarray analysis of pluripotent and multipotent stem cells.  Genes Cells  2010  16:1-11  PubMed ID: 21155951
8674  Hashimoto Y, Adachi S, Matsuno T, Omata K, Yoshitaka Y, Ozeki Y, Umezu Y, Satoh T, Nakamura M.  Effect of an injectable 3D scaffold for osteoblast differentiation depends on bead size.  Biomed Mater Eng  2009  19:391-400  PubMed ID: 20231791



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