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RCB1994 : UV♀2  update : 2018/07/06
CommentMouse vascular endothelial cell line. Transformed by ultraviolet.
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kuribayashi, Kagemasa
Originator Kuribayashi, Kagemasa
Year of deposit 2005
Another name F-2
Animal mouse < Mammals
Strain name (BALB/c×C57BL/6)(CB6)F1
Gender Female
Tissue vascular endothelial cell
Classification transformed
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
2948  Toda K, Tsujioka K, Maruguchi Y, Ishii K, Miyachi Y, Kuribayashi K, Imamura S.  Establishment and characterization of a tumorigenic murine vascular endothelial cell line (F-2).  Cancer Res  1990  50(17):5526-30  PubMed ID: 2386957

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User's Publication
7254  Miura K, Nakaya H, Kobayashi Y.  Experimental assessment of effects of antiproliferative drugs of drug-eluting stents on endothelial cells.  Cardiovasc Revasc Med  2015  16:344-7  PubMed ID: 26253188
7681  Akashi M, Higashi T, Masuda S, Komori T, Furuse M.  A coronary artery disease-associated gene product, JCAD/KIAA1462, is a novel component of endothelial cell-cell junctions.  Biochem. Biophys. Res. Commun.  2011  413:224-9  PubMed ID: 21884682
10776  Nagano F, Mizuno T, Mizumoto S, Yoshioka K, Takahashi K, Tsuboi N, Maruyama S, Yamada S, Nagamatsu T.  Chondroitin sulfate protects vascular endothelial cells from toxicities of extracellular histones.  Eur. J. Pharmacol.  2018  826:48-55  PubMed ID: 29501579
11073  Obata Y, Kita S, Koyama Y, Fukuda S, Takeda H, Takahashi M, Fujishima Y, Nagao H, Masuda S, Tanaka Y, Nakamura Y, Nishizawa H, Funahashi T, Ranscht B, Izumi Y, Bamba T, Fukusaki E, Hanayama R, Shimada S, Maeda N, Shimomura I.  Adiponectin/T-cadherin system enhances exosome biogenesis and decreases cellular ceramides by exosomal release.  JCI Insight  2018    PubMed ID: 29669945
4369  Tsuji-Tamura K, Ogawa M.  Dual inhibition of mTORC1 and mTORC2 perturbs cytoskeletal organization and impairs endothelial cell elongation.  Biochem. Biophys. Res. Commun.  2018    PubMed ID: 29428724



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