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RCB1976 : Ca9-22  update : 2018/08/02
CommentHuman gingival carcinoma cell line. Expressing remarkable EGF receptor. HLA-A 2/24. TKG0485 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0485
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Tissue gingiva
Case history gingival carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0485)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 57

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3304  Kimura Y.  Studies on Lactate dehydrogenase isoenzymes in a cell line (Ca9-22) deribed from carcinoma of the gigiva  Kouku-byo Gakkai-shi  1978  45:20+35 
3306  orikoshi,M., Kimura,Y., Nagura,H., Ono,T., and Ito,H.  A new cell line derived from human carcinoma of the gingiva I. Its establishbint and morphological studies  Nihon Koku Geka Gakkai Zasshi  1974  20:100-106  PubMed ID: 4549822

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User's Publication
7139  Sakaue Y, Domon H, Oda M, Takenaka S, Kubo M, Fukuyama Y, Okiji T, Terao Y.  Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans.  Microbiol. Immunol.  2015  60:10-6  PubMed ID: 26600203
7181  Gojoubori T, Ota H, Kusunoki M, Nishio Y, Nishio K, Iwasa S, Kaneko Y, Asano M.  Electrolytically generated acid functional water inhibits NF-κB activity by attenuating nuclear-cytoplasmic shuttling of p65 and p50 subunits.  J. Recept. Signal Transduct. Res.  2015  36:248-53  PubMed ID: 26480200
7196  Uesawa Y, Sakagami H, Ishihara M, Kagaya H, Kanamoto T, Terakubo S, Nakashima H, Yahagi H, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 3-Styryl-2H-chromenes.  Anticancer Res.  2015  35:5299-307  PubMed ID: 26408690
7722  Kusumoto T, Hamada T, Yamada N, Nagata S, Kanmura Y, Houjou I, Kamikawa Y, Yonezawa S, Sugihara K.  Comprehensive epigenetic analysis using oral rinse samples: a pilot study.  J. Oral Maxillofac. Surg.  2011  70:1486-94  PubMed ID: 21821326
7756  Tamai R, Sugamata M, Kiyoura Y.  Candida albicans enhances invasion of human gingival epithelial cells and gingival fibroblasts by Porphyromonas gingivalis.  Microb. Pathog.  2011  51:250-4  PubMed ID: 21742026
8205  Mori Y, Yano F, Shimohata N, Suzuki S, Chung UI, Takato T.  Trehalose inhibits oral dryness by protecting the cell membrane.  Int J Oral Maxillofac Surg  2010  39:916-21  PubMed ID: 20605407
9558  Pal SK, Nguyen CT, Morita KI, Miki Y, Kayamori K, Yamaguchi A, Sakamoto K.  THBS1 is induced by TGFB1 in the cancer stroma and promotes invasion of oral squamous cell carcinoma.  J. Oral Pathol. Med.  2016  45:730-739  PubMed ID: 26850833
9584  Hino S, Nishiyama A, Matsuta T, Horie N, Shimoyama T, Tanaka S, Sakagami H.  Quest for Cells Responsible for Age-related Increase of Salivary Glycine and Proline.  In Vivo  2016  30:91-7  PubMed ID: 26912818
9604  Chang HS, Tang JY, Yen CY, Huang HW, Wu CY, Chung YA, Wang HR, Chen IS, Huang MY, Chang HW.  Antiproliferation of Cryptocarya concinna-derived cryptocaryone against oral cancer cells involving apoptosis, oxidative stress, and DNA damage.  BMC Complement Altern Med  2016  16:94  PubMed ID: 26955958
9616  Kitajima D, Kasamatsu A, Nakashima D, Miyamoto I, Kimura Y, Saito T, Suzuki T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Tie2 Regulates Tumor Metastasis of Oral Squamous Cell Carcinomas.  J Cancer  2016  7:600-7  PubMed ID: 27053959
9630  Kimura Y, Kasamatsu A, Nakashima D, Yamatoji M, Minakawa Y, Koike K, Fushimi K, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.  J Cancer  2016  7:702-10  PubMed ID: 27076852
9702  Mikami Y, Fukushima A, Komiyama Y, Iwase T, Tsuda H, Higuchi Y, Hayakawa S, Kuyama K, Komiyama K.  Human uterus myoma and gene expression profiling: A novel in vitro model for studying secretory leukocyte protease inhibitor-mediated tumor invasion.  Cancer Lett.  2016  379:84-93  PubMed ID: 27238568
9762  Tachibana H, Sho R, Takeda Y, Zhang X, Yoshida Y, Narimatsu H, Otani K, Ishikawa S, Fukao A, Asao H, Iino M.  Circulating miR-223 in Oral Cancer: Its Potential as a Novel Diagnostic Biomarker and Therapeutic Target.  PLoS ONE  2016  11:e0159693  PubMed ID: 27441818
9783  Khanom R, Nguyen CT, Kayamori K, Zhao X, Morita K, Miki Y, Katsube K, Yamaguchi A, Sakamoto K.  Keratin 17 Is Induced in Oral Cancer and Facilitates Tumor Growth.  PLoS ONE  2016  11:e0161163  PubMed ID: 27512993
9808  Tomikoshi Y, Nomura M, Okudaira N, Sakagami H, Wakabayashi H.  Enhancement of Cytotoxicity of Three Apoptosis-inducing Agents Against Human Oral Squamous Cell Carcinoma Cell Line by Benzoxazinotropone.  In Vivo  2016  30:645-50  PubMed ID: 27566085
9835  Evans M, Murofushi T, Tsuda H, Mikami Y, Zhao N, Ochiai K, Kurita-Ochiai T, Yamamoto M, Otsuka K, Suzuki N.  Combined effects of starvation and butyrate on autophagy-dependent gingival epithelial cell death.  J. Periodont. Res.  2017  52:522-531  PubMed ID: 27624546
9892  Uesawa Y, Sakagami H, Kagaya H, Yamashita M, Takao K, Sugita Y.  Quantitative Structure-cytotoxicity Relationship of 3-Benzylidenechromanones.  Anticancer Res.  2016  36:5803-5812  PubMed ID: 27793902
9911  Fukuchi K, Okudaira N, Adachi K, Odai-Ide R, Watanabe S, Ohno H, Yamamoto M, Kanamoto T, Terakubo S, Nakashima H, Uesawa Y, Kagaya H, Sakagami H.  Antiviral and Antitumor Activity of Licorice Root Extracts.  In Vivo  2016  30:777-785  PubMed ID: 27815461
9915  Tsubaki M, Fujiwara D, Takeda T, Kino T, Tomonari Y, Itoh T, Imano M, Satou T, Sakaguchi K, Nishida S.  The sensitivity of head and neck carcinoma cells to statins is related to the expression of their Ras expression status, and statin-induced apoptosis is mediated via suppression of the Ras/ERK and Ras/mTOR pathways.  Clin. Exp. Pharmacol. Physiol.  2017  44:222-234  PubMed ID: 27805296
9962  Nagano K, Hasegawa Y, Yoshida Y, Yoshimura F.  Comparative analysis of motility and other properties of Treponema denticola strains.  Microb. Pathog.  2017  102:82-88  PubMed ID: 27914958
10148  Takahara T, Kasamatsu A, Yamatoji M, Iyoda M, Kasama H, Saito T, Takeuchi S, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  SIPA1 promotes invasion and migration in human oral squamous cell carcinoma by ITGB1 and MMP7.  Exp. Cell Res.  2017  352:357-363  PubMed ID: 28237246
10155  Koide N, Kasamatsu A, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Evidence for Critical Role of Lymphocyte Cytosolic Protein 1 in Oral Cancer.  Sci Rep  2017  7:43379  PubMed ID: 28230172
10191  Takeuchi S, Kasamatsu A, Yamatoji M, Nakashima D, Endo-Sakamoto Y, Koide N, Takahara T, Shimizu T, Iyoda M, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase.  Biochem. Biophys. Res. Commun.  2017  486:385-390  PubMed ID: 28315328
10197  Sakagami H, Okudaira N, Masuda Y, Amano O, Yokose S, Kanda Y, Suguro M, Natori T, Oizumi H, Oizumi T.  Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin.  Anticancer Res.  2017  37:1023-1029  PubMed ID: 28314260
10201  Sakagami H, Masuda Y, Tomomura M, Yokose S, Uesawa Y, Ikezoe N, Asahara D, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Chalcones.  Anticancer Res.  2017  37:1091-1098  PubMed ID: 28314269
10206  Shimozu Y, Kimura Y, Esumi A, Aoyama H, Kuroda T, Sakagami H, Hatano T.  Ellagitannins of Davidia involucrata. I. Structure of Davicratinic Acid A and Effects of Davidia Tannins on Drug-Resistant Bacteria and Human Oral Squamous Cell Carcinomas.  Molecules  2017  22:  PubMed ID: 28294988
10241  Saijo R, Sekiya H, Tamai E, Kurihara KI, Maki J, Sakagami H, Kawase M.  A Novel Methodology for Synthesis of 1,5,6-Trisubstituted 2(1H)-Pyrazinones of Biological Interest.  Chem. Pharm. Bull.  2017  65:365-372  PubMed ID: 28381677
10344  Takada A, Matsushita K, Horioka S, Furuichi Y, Sumi Y.  Bactericidal effects of 310 nm ultraviolet light-emitting diode irradiation on oral bacteria.  BMC Oral Health  2017  17:96  PubMed ID: 28587675
10377  Takamura T, Suguro H, Mikami Y, Iwase T, Komiyama Y, Kuyama K, Komiyama K, Oki H.  Comparison of gene expression profiles of gingival carcinoma Ca9-22 cells and colorectal adenocarcinoma HT-29 cells to identify potentially important mediators of SLPI-induced cell migration.  J Oral Sci  2017  59:279-287  PubMed ID: 28637988
10386  Suzuki R, Sakagami H, Amano S, Fukuchi K, Sunaga K, Kanamoto T, Terakubo S, Nakashima H, Shirataki Y, Tomomura M, Masuda Y, Yokose S, Tomomura A, Watanabe H, Okawara M, Matahira Y.  Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices.  In Vivo  2017  31:591-598  PubMed ID: 28652425
10418  Kita A, Kasamatsu A, Nakashima D, Endo-Sakamoto Y, Ishida S, Shimizu T, Kimura Y, Miyamoto I, Yoshimura S, Shiiba M, Tanzawa H, Uzawa K.  Activin B Regulates Adhesion, Invasiveness, and Migratory Activities in Oral Cancer: a Potential Biomarker for Metastasis.  J Cancer  2017  8:2033-2041  PubMed ID: 28819404
10517  Murofushi T, Tsuda H, Mikami Y, Yamaguchi Y, Suzuki N.  CAY10591, a SIRT1 activator, suppresses cell growth, invasion, and migration in gingival epithelial carcinoma cells.  J Oral Sci  2017  59:415-423  PubMed ID: 28904318
10526  Ohno T, Yamamoto G, Hayashi JI, Nishida E, Goto H, Sasaki Y, Kikuchi T, Fukuda M, Hasegawa Y, Mogi M, Mitani A.  Angiopoietin-like protein 2 regulates Porphyromonas gingivalis lipopolysaccharide-induced inflammatory response in human gingival epithelial cells.  PLoS ONE  2017  12:e0184825  PubMed ID: 28934245
10572  Uesawa Y, Sakagami H, Ikezoe N, Takao K, Kagaya H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Aurones.  Anticancer Res.  2017  37:6169-6176  PubMed ID: 29061798
10576  Sakagami H, Uesawa Y, Masuda Y, Tomomura M, Yokose S, Miyashiro T, Murai J, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Newly Synthesized Piperic Acid Esters.  Anticancer Res.  2017  37:6161-6168  PubMed ID: 29061797
10682  Yamamoto JI, Kasamatsu A, Okubo Y, Nakashima D, Fushimi K, Minakawa Y, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Evaluation of tryptophan-aspartic acid repeat-containing protein 34 as a novel tumor-suppressor molecule in human oral cancer.  Biochem. Biophys. Res. Commun.  2018  495:2469-2474  PubMed ID: 29278705
10683  Suzuki S, Hoshino H, Yoshida K, Nakanishi J, Tsuchiya-Hirata S, Kobuke S, Haruyama N, Nishimura F, Shiba H.  Genome-wide identification of chromatin-enriched RNA reveals that unspliced dentin matrix protein-1 mRNA regulates cell proliferation in squamous cell carcinoma.  Biochem. Biophys. Res. Commun.  2018  495:2303-2309  PubMed ID: 29278708
10724  Uesawa Y, Sakagami H, Okudaira N, Toda K, Takao K, Kagaya H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Cinnamic Acid Phenetyl Esters.  Anticancer Res.  2018  38:817-823  PubMed ID: 29374707
10727  Sakagami H, Okudaira N, Uesawa Y, Takao K, Kagaya H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 2-Azolylchromones.  Anticancer Res.  2018  38:763-770  PubMed ID: 29374700
10806  Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.  Exp. Cell Res.  2018  367:112-118  PubMed ID: 29614312
10820  Fukamachi M, Kasamatsu A, Endo-Sakamoto Y, Fushimi K, Kasama H, Iyoda M, Minakawa Y, Shiiba M, Tanzawa H, Uzawa K.  Multiple coagulation factor deficiency protein 2 as a crucial component in metastasis of human oral cancer.  Exp. Cell Res.  2018  368:119-125  PubMed ID: 29679592
10829  Wada T, Maruyama R, Irie Y, Hashimoto M, Wakabayashi H, Okudaira N, Uesawa Y, Kagaya H, Sakagami H.  In Vitro Anti-tumor Activity of Azulene Amide Derivatives.  In Vivo  2018  32:479-486  PubMed ID: 29695549
10937    Effect of Micro/Nano-Patterned Surfaces on Cell Adhesion of Ca9-22 cells  e-J. Surf. Sci. Nanotech.  2017  15 : 1-6 
10958    Inactivation of dermatopontin via histone deacetylation in human oral cancer  Journal of Oral and Maxillofacial Surgery, Medicine, and Pathology   2017  29, 400-404 
11086  Uesawa Y, Sakagami H, Shi H, Hirose M, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Furo[2,3-b]chromones.  Anticancer Res.  2018    PubMed ID: 29848675
11147  Nagai J, Shi H, Kubota Y, Bandow K, Okudaira N, Uesawa Y, Sakagami H, Tomomura M, Tomomura A, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Pyrano[4,3-b]chromones.  Anticancer Res.  2018    PubMed ID: 30061209
11148  Shi H, Nagai J, Sakatsume T, Bandow K, Okudaira N, Uesawa Y, Sakagami H, Tomomura M, Tomomura A, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 3-(N-Cyclicamino)chromone Derivatives.  Anticancer Res.  2018    PubMed ID: 30061210
11226  Fukushima R, Kasamatsu A, Nakashima D, Higo M, Fushimi K, Kasama H, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.  J Cancer  2018    PubMed ID: 30271493
11354  Nakamura Y, Nakahata S, Kondo Y, Izumi A, Yamamoto K, Ichikawa T, Tamura T, Noumi K, Yamashita Y, Morishita K.  Overexpression of absent in melanoma 2 in oral squamous cell carcinoma contributes to tumor progression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30587341
11377  Inoue K, Hatano K, Hanamatsu Y, Saigo C, Kito Y, Bunai K, Shibata T, Takeuchi T.  Pathobiological role of cleft palate transmembrane protein 1 family proteins in oral squamous cell carcinoma.  J. Cancer Res. Clin. Oncol.  2019    PubMed ID: 30635792
4078  Halise Inci, Cem Yamali, Gulsen Gunesacar, Hiroshi Sakagami, Noriyuki Okudaira, Yoshihiro Uesawa, Hajime Kagaya  Cytotoxicity, apoptosis, and QSAR studies of phenothiazine derived methoxylated chalcones as anticancer drug candidates  Medicinal Chemistry Research  2018  27:2366–2378 
4172  Tamai R, Kobayashi-Sakamoto M, Kiyoura Y.  Extracellular galectin-1 enhances adhesion to and invasion of oral epithelial cells by Porphyromonas gingivalis.  Can. J. Microbiol.  2018    PubMed ID: 29544077
4173  Uehara M, Minemura H, Ohno T, Hashimoto M, Wakabayashi H, Okudaira N, Sakagami H.  In Vitro Antitumor Activity of Alkylaminoguaiazulenes.  In Vivo  2018    PubMed ID: 29695558
4236  Nanbu T, Umemura N, Ohkoshi E, Nanbu K, Sakagami H, Shimada J.  Combined SN-38 and gefitinib treatment promotes CD44 degradation in head and neck squamous cell carcinoma cells.  Oncol. Rep.  2018    PubMed ID: 29192320
4237  Yamanouchi R, Harada K, Ferdous T, Ueyama Y.  Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma.  Mol Clin Oncol  2018    PubMed ID: 29456845
4345  Hou B, Ishinaga H, Midorikawa K, Nakamura S, Hiraku Y, Oikawa S, Ma N, Takeuchi K, Murata M.  Let-7c inhibits migration and epithelial-mesenchymal transition in head and neck squamous cell carcinoma by targeting IGF1R and HMGA2.  Oncotarget  2018    PubMed ID: 29507664
4574  Yasui H, Ohnishi Y, Nakajima M, Nozaki M.  Migration of oral squamous cell carcinoma cells are induced by HGF/c-Met signalling via lamellipodia and filopodia formation.  Oncol Rep  2017  37(6):3674-3680  PubMed ID: 28440510

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