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RCB1975 : HSC-3  update : 2018/08/02
CommentHuman tongue squamous cell carcinoma cell line. HLA-A 2/24. TKG0484 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Momose, F.
Year of deposit 2004
Original cell TKG0484
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 64 years
Tissue tongue
Case history Squamous cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0484)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 3
User's Publication 55

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3090  Rikimaru K, Toda H, Tachikawa N, Kamata N, Enomoto S.  Growth of the malignant and nonmalignant human squamous cells in a protein-free defined medium.  In Vitro Cell Dev Biol  1990  26:849-856  PubMed ID: 2228902
3248  Ichijo H, Momose F, Miyazono K  Biological effects and binding properties of transforming growth factor-beta on human oral squamous cell carcinoma cells.  Exp Cell Res  1990  187:263-9  PubMed ID: 2156718
3243  Momose F, Araida T, Negishi A, Ichijo H, Shioda S, Sasaki S  Variant sublines with different metastatic potentials selected in nude mice from human oral squamous cell carcinomas.  J Oral Pathol Med  1989  18:391-5  PubMed ID: 2585303

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User's Publication
7096  Yoshino F, Yoshida A, Toyama T, Wada-T S, Takahashi S  α-Glucosyl hesperidin suppressed the exacerbation of 5-fluorouracil-induced oral mucositis in the hamster cheek pouch.  J Functional Foods  2016  21:223-231 
7109  Miyazaki Y, Yoshida N, Nozaki T, Inoue H, Kikuchi K, Kusama K.  Telomerase activity in the occurrence and progression of oral squamous cell carcinoma.  J Oral Sci  2015  57:295-303  PubMed ID: 26666852
7175  Ohnishi Y, Yasui H, Kakudo K, Nozaki M.  Cetuximab-resistant oral squamous cell carcinoma cells become sensitive in anchorage-independent culture conditions through the activation of the EGFR/AKT pathway.  Int. J. Oncol.  2015  47:2165-72  PubMed ID: 26497980
7196  Uesawa Y, Sakagami H, Ishihara M, Kagaya H, Kanamoto T, Terakubo S, Nakashima H, Yahagi H, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 3-Styryl-2H-chromenes.  Anticancer Res.  2015  35:5299-307  PubMed ID: 26408690
7601  Uesawa Y, Mohri K, Kawase M, Ishihara M, Sakagami H.  Quantitative structure-activity relationship (QSAR) analysis of tumor-specificity of 1,2,3,4-tetrahydroisoquinoline derivatives.  Anticancer Res.  2011  31:4231-8  PubMed ID: 22199286
7691  Susuki D, Kimura S, Naganuma S, Tsuchiyama K, Tanaka T, Kitamura N, Fujieda S, Itoh H.  Regulation of microRNA expression by hepatocyte growth factor in human head and neck squamous cell carcinoma.  Cancer Sci.  2011  102:2164-71  PubMed ID: 21899661
7722  Kusumoto T, Hamada T, Yamada N, Nagata S, Kanmura Y, Houjou I, Kamikawa Y, Yonezawa S, Sugihara K.  Comprehensive epigenetic analysis using oral rinse samples: a pilot study.  J. Oral Maxillofac. Surg.  2011  70:1486-94  PubMed ID: 21821326
8085  Sakagami H, Zhou L, Kawano M, Thet MM, Tanaka S, Machino M, Amano S, Kuroshita R, Watanabe S, Chu Q, Wang QT, Kanamoto T, Terakubo S, Nakashima H, Sekine K, Shirataki Y, Zhang CH, Uesawa Y, Mohri K, Kitajima M, Oizumi H, Oizumi T.  Multiple biological complex of alkaline extract of the leaves of Sasa senanensis Rehder.  In Vivo  2010  24:735-43  PubMed ID: 20952742
8383  Uemura N, Kodama S, Nomi N, Okamoto T, Suzuki M.  Correlation between anti-survivin antibody and survivin mRNA expression in head and neck cancer patients.  Acta Otolaryngol.  2010  130:959-65  PubMed ID: 20100132
9584  Hino S, Nishiyama A, Matsuta T, Horie N, Shimoyama T, Tanaka S, Sakagami H.  Quest for Cells Responsible for Age-related Increase of Salivary Glycine and Proline.  In Vivo  2016  30:91-7  PubMed ID: 26912818
9616  Kitajima D, Kasamatsu A, Nakashima D, Miyamoto I, Kimura Y, Saito T, Suzuki T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Tie2 Regulates Tumor Metastasis of Oral Squamous Cell Carcinomas.  J Cancer  2016  7:600-7  PubMed ID: 27053959
9630  Kimura Y, Kasamatsu A, Nakashima D, Yamatoji M, Minakawa Y, Koike K, Fushimi K, Higo M, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  ARNT2 Regulates Tumoral Growth in Oral Squamous Cell Carcinoma.  J Cancer  2016  7:702-10  PubMed ID: 27076852
9654  Kumasaka A, Matsumoto N, Mukae S, Kitano T, Noguchi H, Ohki H, Komiyama K, Ando T.  Rapid and Specific Screening Assay for KRAS Oncogene Mutation by a Novel Gene Amplification Method.  Anticancer Res.  2016  36:1571-9  PubMed ID: 27069133
9676  Shibata A, Ibaragi S, Mandai H, Tsumura T, Kishimoto K, Okui T, Hassan NM, Shimo T, Omori K, Hu GF, Takashiba S, Suga S, Sasaki A.  Synthetic Terrein Inhibits Progression of Head and Neck Cancer by Suppressing Angiogenin Production.  Anticancer Res.  2016  36:2161-8  PubMed ID: 27127118
9694  Harada K, Ferdous T, Harada T, Ueyama Y.  Metformin in combination with 5-fluorouracil suppresses tumor growth by inhibiting the Warburg effect in human oral squamous cell carcinoma.  Int. J. Oncol.  2016  49:276-84  PubMed ID: 27210058
9762  Tachibana H, Sho R, Takeda Y, Zhang X, Yoshida Y, Narimatsu H, Otani K, Ishikawa S, Fukao A, Asao H, Iino M.  Circulating miR-223 in Oral Cancer: Its Potential as a Novel Diagnostic Biomarker and Therapeutic Target.  PLoS ONE  2016  11:e0159693  PubMed ID: 27441818
9808  Tomikoshi Y, Nomura M, Okudaira N, Sakagami H, Wakabayashi H.  Enhancement of Cytotoxicity of Three Apoptosis-inducing Agents Against Human Oral Squamous Cell Carcinoma Cell Line by Benzoxazinotropone.  In Vivo  2016  30:645-50  PubMed ID: 27566085
9851  Masui A, Hamada M, Kameyama H, Wakabayashi K, Takasu A, Imai T, Iwai S, Yura Y.  Autophagy as a Survival Mechanism for Squamous Cell Carcinoma Cells in Endonuclease G-Mediated Apoptosis.  PLoS ONE  2016  11:e0162786  PubMed ID: 27658240
9892  Uesawa Y, Sakagami H, Kagaya H, Yamashita M, Takao K, Sugita Y.  Quantitative Structure-cytotoxicity Relationship of 3-Benzylidenechromanones.  Anticancer Res.  2016  36:5803-5812  PubMed ID: 27793902
9896  Suzuki R, Matsushima Y, Okudaira N, Sakagami H, Shirataki Y.  Cytotoxic Components Against Human Oral Squamous Cell Carcinoma Isolated from Andrographis paniculata.  Anticancer Res.  2016  36:5931-5935  PubMed ID: 27793918
9911  Fukuchi K, Okudaira N, Adachi K, Odai-Ide R, Watanabe S, Ohno H, Yamamoto M, Kanamoto T, Terakubo S, Nakashima H, Uesawa Y, Kagaya H, Sakagami H.  Antiviral and Antitumor Activity of Licorice Root Extracts.  In Vivo  2016  30:777-785  PubMed ID: 27815461
10044  Ohkoshi E, Umemura N.  Induced overexpression of CD44 associated with resistance to apoptosis on DNA damage response in human head and neck squamous cell carcinoma cells.  Int. J. Oncol.  2017  50:387-395  PubMed ID: 28035370
10148  Takahara T, Kasamatsu A, Yamatoji M, Iyoda M, Kasama H, Saito T, Takeuchi S, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  SIPA1 promotes invasion and migration in human oral squamous cell carcinoma by ITGB1 and MMP7.  Exp. Cell Res.  2017  352:357-363  PubMed ID: 28237246
10191  Takeuchi S, Kasamatsu A, Yamatoji M, Nakashima D, Endo-Sakamoto Y, Koide N, Takahara T, Shimizu T, Iyoda M, Ogawara K, Shiiba M, Tanzawa H, Uzawa K.  TEAD4-YAP interaction regulates tumoral growth by controlling cell-cycle arrest at the G1 phase.  Biochem. Biophys. Res. Commun.  2017  486:385-390  PubMed ID: 28315328
10197  Sakagami H, Okudaira N, Masuda Y, Amano O, Yokose S, Kanda Y, Suguro M, Natori T, Oizumi H, Oizumi T.  Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin.  Anticancer Res.  2017  37:1023-1029  PubMed ID: 28314260
10201  Sakagami H, Masuda Y, Tomomura M, Yokose S, Uesawa Y, Ikezoe N, Asahara D, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Chalcones.  Anticancer Res.  2017  37:1091-1098  PubMed ID: 28314269
10206  Shimozu Y, Kimura Y, Esumi A, Aoyama H, Kuroda T, Sakagami H, Hatano T.  Ellagitannins of Davidia involucrata. I. Structure of Davicratinic Acid A and Effects of Davidia Tannins on Drug-Resistant Bacteria and Human Oral Squamous Cell Carcinomas.  Molecules  2017  22:  PubMed ID: 28294988
10241  Saijo R, Sekiya H, Tamai E, Kurihara KI, Maki J, Sakagami H, Kawase M.  A Novel Methodology for Synthesis of 1,5,6-Trisubstituted 2(1H)-Pyrazinones of Biological Interest.  Chem. Pharm. Bull.  2017  65:365-372  PubMed ID: 28381677
10386  Suzuki R, Sakagami H, Amano S, Fukuchi K, Sunaga K, Kanamoto T, Terakubo S, Nakashima H, Shirataki Y, Tomomura M, Masuda Y, Yokose S, Tomomura A, Watanabe H, Okawara M, Matahira Y.  Evaluation of Biological Activity of Mastic Extracts Based on Chemotherapeutic Indices.  In Vivo  2017  31:591-598  PubMed ID: 28652425
10576  Sakagami H, Uesawa Y, Masuda Y, Tomomura M, Yokose S, Miyashiro T, Murai J, Takao K, Kanamoto T, Terakubo S, Kagaya H, Nakashima H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Newly Synthesized Piperic Acid Esters.  Anticancer Res.  2017  37:6161-6168  PubMed ID: 29061797
10649  Koshizuka K, Hanazawa T, Kikkawa N, Katada K, Okato A, Arai T, Idichi T, Osako Y, Okamoto Y, Seki N.  Antitumor miR-150-5p and miR-150-3p inhibit cancer cell aggressiveness by targeting SPOCK1 in head and neck squamous cell carcinoma.  Auris Nasus Larynx  2018  45:854-865  PubMed ID: 29233721
10664  Ohnishi Y, Yasui H, Nozaki M, Nakajima M.  Molecularly-targeted therapy for the oral cancer stem cells.  Jpn Dent Sci Rev  2018  54:88-103  PubMed ID: 29755619
10668  Ikehata N, Takanashi M, Satomi T, Watanabe M, Hasegawa O, Kono M, Enomoto A, Chikazu D, Kuroda M.  Toll-like receptor 2 activation implicated in oral squamous cell carcinoma development.  Biochem. Biophys. Res. Commun.  2018  495:2227-2234  PubMed ID: 29269299
10691  Iwai S, Kishimoto S, Amano Y, Nishiguchi A, Matsusaki M, Takeshita A, Akashi M.  Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion.  J Biomed Mater Res A  2017    PubMed ID: 29280265
10724  Uesawa Y, Sakagami H, Okudaira N, Toda K, Takao K, Kagaya H, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of Cinnamic Acid Phenetyl Esters.  Anticancer Res.  2018  38:817-823  PubMed ID: 29374707
10806  Okubo Y, Kasamatsu A, Yamatoji M, Fushimi K, Ishigami T, Shimizu T, Kasama H, Shiiba M, Tanzawa H, Uzawa K.  Diacylglycerol lipase alpha promotes tumorigenesis in oral cancer by cell-cycle progression.  Exp. Cell Res.  2018  367:112-118  PubMed ID: 29614312
10829  Wada T, Maruyama R, Irie Y, Hashimoto M, Wakabayashi H, Okudaira N, Uesawa Y, Kagaya H, Sakagami H.  In Vitro Anti-tumor Activity of Azulene Amide Derivatives.  In Vivo  2018  32:479-486  PubMed ID: 29695549
11036    Increased Expression of CD44v9, A Cancer Stem Cell Marker, in Head And Neck Squamous Cell Carcinoma Cells after Irradiation  Int J Cancer Oncol  2017  4: 225- 230 
11110  Hara Y, Sakagami H, Shi H, Abe T, Tamura N, Takeshima H, Horie N, Kaneko T, Shiratsuchi H, Kaneko T.  Partial Protection of Paclitaxel-induced Neurotoxicity by Antioxidants.  In Vivo  2018    PubMed ID: 29936454
11115  Shi H, Nagai J, Sakatsume T, Bandow K, Okudaira N, Sakagami H, Tomomura M, Tomomura A, Uesawa Y, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 2-(N-cyclicamino)chromone Derivatives.  Anticancer Res.  2018    PubMed ID: 29970510
11148  Shi H, Nagai J, Sakatsume T, Bandow K, Okudaira N, Uesawa Y, Sakagami H, Tomomura M, Tomomura A, Takao K, Sugita Y.  Quantitative Structure-Cytotoxicity Relationship of 3-(N-Cyclicamino)chromone Derivatives.  Anticancer Res.  2018    PubMed ID: 30061210
11226  Fukushima R, Kasamatsu A, Nakashima D, Higo M, Fushimi K, Kasama H, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K.  Overexpression of Translocation Associated Membrane Protein 2 Leading to Cancer-Associated Matrix Metalloproteinase Activation as a Putative Metastatic Factor for Human Oral Cancer.  J Cancer  2018    PubMed ID: 30271493
11300  Shimizu R, Ibaragi S, Eguchi T, Kuwajima D, Kodama S, Nishioka T, Okui T, Obata K, Takabatake K, Kawai H, Ono K, Okamoto K, Nagatsuka H, Sasaki A.  Nicotine promotes lymph node metastasis and cetuximab resistance in head and neck squamous cell carcinoma.  Int. J. Oncol.  2019    PubMed ID: 30431077
11306  Kakuguchi W, Nomura T, Kitamura T, Otsuguro S, Matsushita K, Sakaitani M, Maenaka K, Tei K.  Suramin, screened from an approved drug library, inhibits HuR functions and attenuates malignant phenotype of oral cancer cells.  Cancer Med  2018    PubMed ID: 30449075
11350  Katase N, Nishimatsu SI, Yamauchi A, Yamamura M, Fujita S.  DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways.  Int. J. Oncol.  2019    PubMed ID: 30569110
11354  Nakamura Y, Nakahata S, Kondo Y, Izumi A, Yamamoto K, Ichikawa T, Tamura T, Noumi K, Yamashita Y, Morishita K.  Overexpression of absent in melanoma 2 in oral squamous cell carcinoma contributes to tumor progression.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 30587341
4173  Uehara M, Minemura H, Ohno T, Hashimoto M, Wakabayashi H, Okudaira N, Sakagami H.  In Vitro Antitumor Activity of Alkylaminoguaiazulenes.  In Vivo  2018    PubMed ID: 29695558
4235  Okazaki S, Shintani S, Hirata Y, Suina K, Semba T, Yamasaki J, Umene K, Ishikawa M, Saya H, Nagano O.  Synthetic lethality of the ALDH3A1 inhibitor dyclonine and xCT inhibitors in glutathione deficiency-resistant cancer cells.  Oncotarget  2018    PubMed ID: 30333913
4236  Nanbu T, Umemura N, Ohkoshi E, Nanbu K, Sakagami H, Shimada J.  Combined SN-38 and gefitinib treatment promotes CD44 degradation in head and neck squamous cell carcinoma cells.  Oncol. Rep.  2018    PubMed ID: 29192320
4237  Yamanouchi R, Harada K, Ferdous T, Ueyama Y.  Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma.  Mol Clin Oncol  2018    PubMed ID: 29456845
4238  Sumi S, Umemura N, Adachi M, Ohta T, Naganawa K, Kawaki H, Takayama E, Kondoh N, Sumitomo S.  The luminance ratio of autofluorescence in a xenograft mouse model is stable through tumor growth stages.  Clin Exp Dent Res  2018    PubMed ID: 30386639
4239  Hong Y, He H, Sui W, Zhang J, Zhang S, Yang D.  Long non-coding RNA H1 promotes cell proliferation and invasion by acting as a ceRNA of miR‑138 and releasing EZH2 in oral squamous cell carcinoma.  Int. J. Oncol.  2018    PubMed ID: 29344674
4240  Jiaranuchart S, Kaida A, Onozato Y, Harada H, Miura M.  DNA damage response following X-irradiation in oral cancer cell lines HSC3 and HSC4.  Arch. Oral Biol.  2018    PubMed ID: 29522917
4241  Sumita Y, Yamazaki M, Maruyama S, Abé T, Cheng J, Takagi R, Tanuma JI.  Cytoplasmic expression of SOX9 as a poor prognostic factor for oral squamous cell carcinoma.  Oncol. Rep.  2018    PubMed ID: 30132562
4242  Daigo K, Takano A, Thang PM, Yoshitake Y, Shinohara M, Tohnai I, Murakami Y, Maegawa J, Daigo Y.  Characterization of KIF11 as a novel prognostic biomarker and therapeutic target for oral cancer.  Int. J. Oncol.  2018    PubMed ID: 29115586

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