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細胞番号 : 細胞名
RCB1973 : PK-45H  update : 2023/01/04
細胞特性(Comment:英)Human pancreatic carcinoma cell line, the same patient as RCB2141. TKG0491 (Deposited from Tohoku Univ.).
細胞特性(日)ヒト膵臓癌由来細胞株。RCB2141と同一患者由来。 TKG0491 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Obinata, Masuo
樹立者 Kobori, M.
寄託日 2004
元の細胞 TKG0491
動物種 _human < Mammals
属名 Homo
種名 sapiens
採取組織 pancreas
病名 pancreas carcinoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0491)
細胞寿命 infinite
細胞形態 epithelial-like
Cellosaurus(Expasy) CVCL_6748
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 RPMI1640 + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 0件
Reference(日) 0件
利用者成果(英) 33件
利用者成果(日) 0件

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利用者成果(英)
18286  Amada K, Hijiya N, Ikarimoto S, Yanagihara K, Hanada T, Hidano S, Kurogi S, Tsukamoto Y, Nakada C, Kinoshita K, Hirashita Y, Uchida T, Shin T, Yada K, Hirashita T, Kobayashi T, Murakami K, Inomata M, Shirao K, Aoki M, Takekawa M, Moriyama M.  Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor  Cancer Sci  2023  114(5):2189-2202  PubMed ID: 36694355   DOI: 10.1111/cas.15735
19720  Hirose S, Waku T, Tani M, Masuda H, Endo K, Ashitani S, Aketa I, Kitano H, Nakada S, Wada A, Hatanaka A, Osawa T, Soga T, Kobayashi A.  NRF3 activates mTORC1 arginine-dependently for cancer cell viability  iScience  2023  26(2):106045  PubMed ID: 36818298   DOI: 10.1016/j.isci.2023.106045
19722  Masuda T, Fukuda A, Yamakawa G, Omatsu M, Namikawa M, Sono M, Fukunaga Y, Nagao M, Araki O, Yoshikawa T, Ogawa S, Masuo K, Goto N, Hiramatsu Y, Muta Y, Tsuda M, Maruno T, Nakanishi Y, Masui T, Hatano E, Matsuzaki T, Noda M, Seno H.  Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis  J Clin Invest  2023  133(18):e161847  PubMed ID: 37712427   DOI: 10.1172/JCI161847
20570  Vaes RDW, van Dijk DPJ, Farshadi EA, Olde Damink SWM, Rensen SS, Langen RC.  Human pancreatic tumour organoid-derived factors enhance myogenic differentiation.  J Cachexia Sarcopenia Muscle  2022    PubMed ID: 35146962   DOI: 10.1002/jcsm.12917
20431  Shiozaki A, Konishi T, Kosuga T, Kudou M, Kurashima K, Inoue H, Shoda K, Arita T, Konishi H, Morimura R, Komatsu S, Ikoma H, Toma A, Kubota T, Fujiwara H, Okamoto K, Otsuji E.  Roles of voltage‑gated potassium channels in the maintenance of pancreatic cancer stem cells.  Int J Oncol  2021  59(4)  PubMed ID: 34414448   DOI: 10.3892/ijo.2021.5256
11820  Okazaki J, Tanahashi T, Sato Y, Miyoshi J, Nakagawa T, Kimura T, Miyamoto H, Fujino Y, Nakamura F, Takehara M, Ma B, Bando M, Kitamura S, Okamoto K, Muguruma N, Sogabe M, Takayama T.  MicroRNA-296-5p Promotes Cell Invasion and Drug Resistance by Targeting Bcl2-Related Ovarian Killer, Leading to a Poor Prognosis in Pancreatic Cancer.  Digestion  2020  101(6):794-806  PubMed ID: 31563901   DOI: 10.1159/000503225
13088  Hirashita T, Hirashita Y, Iwashita Y, Endo Y, Kiyonaga M, Matsumoto S, Hijiya N, Moriyama M, Murakami K, Inomata M.  S6 ribosomal protein phosphorylation is associated with malignancy of intraductal papillary mucinous neoplasm of the pancreas.  Ann Gastroenterol Surg  2020    PubMed ID: 33005852   DOI: 10.1002/ags3.12367
13240  Onodera T, Momose I, Adachi H, Yamazaki Y, Sawa R, Ohba SI, Kawada M.  Human pancreatic cancer cells under nutrient deprivation are vulnerable to redox system inhibition.  J Biol Chem  2020    PubMed ID: 32978257   DOI: 10.1074/jbc.RA120.013893
13542  Takehara M, Sato Y, Kimura T, Noda K, Miyamoto H, Fujino Y, Miyoshi J, Nakamura F, Wada H, Bando Y, Ikemoto T, Shimada M, Muguruma N, Takayama T.  Cancer-associated adipocytes promote pancreatic cancer progression through SAA1 expression  Cancer Sci  2020  111(8):2883-2894  PubMed ID: 32535957   DOI: 10.1111/cas.14527
5027  Nishizawa N1, Harada H1, Kumamoto Y1, Kaizu T, Katoh H, Tajima H, Ushiku H, Yokoi K, Igarashi K, Fujiyama Y, Okuwaki K, Iwai T, Watanabe M, Yamashita K1  Diagnostic potential of hypermethylation of the cysteine dioxygenase 1 gene (CDO1) promoter DNA in pancreatic cancer.  Cancer Sci  2019  110(9):2846-2855  PubMed ID: 31325200   DOI: 10.1111/cas.14134
11811  Mohammad GH, Vassileva V, Acedo P, Olde Damink SWM, Malago M, Dhar DK, Pereira SP.  Targeting Pyruvate Kinase M2 and Lactate Dehydrogenase A Is an Effective Combination Strategy for the Treatment of Pancreatic Cancer.  Cancers (Basel)  2019  11(9):1372  PubMed ID: 31527446   DOI: 10.3390/cancers11091372
9183  Maria New, Tim Van Acker Jun-Ichi Sakamaki, Ming Jiang, Rebecca E Saunders, Jaclyn Long, Victoria M-Y Wang, Axel Behrens, Joana Cerveira, Padhmanand Sudhakar, Tamas Korcsmaros, Harold B J Jefferies, Kevin M Ryan, Michael Howell, Sharon A Tooze  MDH1 and MPP7 Regulate Autophagy in Pancreatic Ductal Adenocarcinoma  Cancer Res  2019  79 (8), 1884-1898  PubMed ID: 30765601   DOI: 10.1158/0008-5472.CAN-18-2553
11161  Chiba M, Kubota S, Sato K, Monzen S.  Exosomes released from pancreatic cancer cells enhance angiogenic activities via dynamin-dependent endocytosis in endothelial cells in vitro.  Sci Rep  2018  8(1):11972  PubMed ID: 30097593   DOI: 10.1038/s41598-018-30446-1
4440  Mitsuru Chiba1, Chihiro Kawamura, Shiori Kubota, Konomi Sato, Ayaka Sakai  Exosomes released from pancreatic cancer cells are heterogeneous particle populations.    2018  29 : 2988-2991    DOI: 10.4066/biomedicalresearch.29-18-782
4323  Chiba M, Kubota S, Sakai A, Monzen S.  Cell‑to‑cell communication via extracellular vesicles among human pancreatic cancer cells derived from the same patient.  Mol Med Rep  2018    PubMed ID: 30106154   DOI: 10.3892/mmr.2018.9376
10320  Kasai S, Sasaki T, Watanabe A, Nishiya M, Yasuhira S, Shibazaki M, Maesawa C.  Bcl-2/Bcl-xL inhibitor ABT-737 sensitizes pancreatic ductal adenocarcinoma to paclitaxel-induced cell death.  Oncol Lett  2017  14:903-908  PubMed ID: 28693250   DOI: 10.3892/ol.2017.6211
14232  Yasunaga M, Manabe S, Matsumura Y.  Immunoregulation by IL-7R-targeting antibody-drug conjugates: overcoming steroid-resistance in cancer and autoimmune disease  Sci Rep  2017  7(1):10735  PubMed ID: 28878234   DOI: 10.1038/s41598-017-11255-4
14726  Imamura T, Komatsu S, Ichikawa D, Miyamae M, Okajima W, Ohashi T, Kiuchi J, Nishibeppu K, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Depleted tumor suppressor miR-107 in plasma relates to tumor progression and is a novel therapeutic target in pancreatic cancer  Sci Rep  2017  7(1):5708  PubMed ID: 28720759   DOI: 10.1038/s41598-017-06137-8
9592  Hijiya N, Tsukamoto Y, Nakada C, Tung Nguyen L, Kai T, Matsuura K, Shibata K, Inomata M, Uchida T, Tokunaga A, Amada K, Shirao K, Yamada Y, Mori H, Takeuchi I, Seto M, Aoki M, Takekawa M, Moriyama M.  Genomic Loss of DUSP4 Contributes to the Progression of Intraepithelial Neoplasm of Pancreas to Invasive Carcinoma.  Cancer Res.  2016  76:2612-25  PubMed ID: 26941286   DOI: 10.1158/0008-5472.CAN-15-1846
9938  Komura D, Isagawa T, Kishi K, Suzuki R, Sato R, Tanaka M, Katoh H, Yamamoto S, Tatsuno K, Fukayama M, Aburatani H, Ishikawa S.  CASTIN: a system for comprehensive analysis of cancer-stromal interactome.  BMC Genomics  2016  17:899  PubMed ID: 27829362   DOI: 10.1186/s12864-016-3207-z
12659  Mohammad GH, Olde Damink SW, Malago M, Dhar DK, Pereira SP.  Pyruvate Kinase M2 and Lactate Dehydrogenase A Are Overexpressed in Pancreatic Cancer and Correlate with Poor Outcome  PLoS One  2016  11(3):e0151635  PubMed ID: 26989901   DOI: 10.1371/journal.pone.0151635
7168  Miyamae M, Komatsu S, Ichikawa D, Kawaguchi T, Hirajima S, Okajima W, Ohashi T, Imamura T, Konishi H, Shiozaki A, Morimura R, Ikoma H, Ochiai T, Okamoto K, Taniguchi H, Otsuji E.  Plasma microRNA profiles: identification of miR-744 as a novel diagnostic and prognostic biomarker in pancreatic cancer.  Br. J. Cancer  2015  113:1467-76  PubMed ID: 26505678   DOI: 10.1038/bjc.2015.366
5019  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  SU11274 suppresses proliferation and motility of pancreatic cancer cells.  Oncol Lett  2015  10(3):1468-1472  PubMed ID: 26622692   DOI: 10.3892/ol.2015.3452
5018  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Frizzled-2: A potential novel target for molecular pancreatic cancer therapy.  Oncol Lett  2014  7(1):74-78  PubMed ID: 24348824   DOI: 10.3892/ol.2013.1681
5024  Kawaguchi T, Komatsu S, Ichikawa D, Morimura R, Tsujiura M, Konishi H, Takeshita H, Nagata H, Arita T, Hirajima S, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Clinical impact of circulating miR-221 in plasma of patients with pancreatic cancer.  Br J Cancer  2013  108(2):361-9  PubMed ID: 23329235   DOI: 10.1038/bjc.2012.546
13745  Masahiro Sato et al  Mosaic Expression of Pluripotency-Related Proteins Oct-3/4 and Alkaline Phosphatase in Human Pancreatic Carcinoma Cell PANC-1  Advanced Studies in Biology  2013  5:157-172   
5020  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  nsulin-like growth factor I receptor involvement in proliferation of NOR-P1 cells in serum-free media.  J Cell Biochem  2012  113(8):2714-20  PubMed ID: 22441995   DOI: 10.1002/jcb.24149
12868  Yoshida M, Takimoto R, Murase K, Sato Y, Hirakawa M, Tamura F, Sato T, Iyama S, Osuga T, Miyanishi K, Takada K, Hayashi T, Kobune M, Kato J.  Targeting anticancer drug delivery to pancreatic cancer cells using a fucose-bound nanoparticle approach  PLoS One  2012  7(7):e39545  PubMed ID: 22808043   DOI: 10.1371/journal.pone.0039545
7643  Morimura R, Komatsu S, Ichikawa D, Takeshita H, Tsujiura M, Nagata H, Konishi H, Shiozaki A, Ikoma H, Okamoto K, Ochiai T, Taniguchi H, Otsuji E.  Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer.  Br. J. Cancer  2011  105:1733-40  PubMed ID: 22045190   DOI: 10.1038/bjc.2011.453
8294  Tomizawa M, Shinozaki F, Sugiyama T, Yamamoto S, Sueishi M, Yoshida T.  Insulin-like growth factor-I receptor in proliferation and motility of pancreatic cancer.  World J. Gastroenterol.  2010  16:1854-8  PubMed ID: 20397262   DOI: 10.3748/wjg.v16.i15.1854
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
18311  Mitsuoka K, Miyoshi S, Kato Y, Murakami Y, Utsumi R, Kubo Y, Noda A, Nakamura Y, Nishimura S, Tsuji A.  Cancer detection using a PET tracer, 11C-glycylsarcosine, targeted to H+/peptide transporter  J Nucl Med  2008  49(4):615-22  PubMed ID: 18344442   DOI: 10.2967/jnumed.107.048231
5016  Inami K1, Kajino K, Abe M, Hagiwara Y, Maeda M, Suyama M, Watanabe S, Hino O.  Secretion of N-ERC/mesothelin and expression of C-ERC/mesothelin in human pancreatic ductal carcinoma.  Oncol Rep  2008  20(6):1375-80  PubMed ID: 19020717  

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