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RCB1967 : LU65  update : 2018/08/02
CommentHuman lung carcinoma cell line. TKG0442 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0442
Animal human < Mammals
Genus Homo
Species sapiens
Tissue lung
Case history Lung small cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0442)
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.02% EDTA
Culture information Passage ratio 1 : 4-6 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 3
User's Publication 5


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Reference
3440  Suzuki K, Miyasaka H, Ota H, Yamakawa Y, Tagawa M, Kuramoto A, Mizuno S.  Purification and partial primary sequence of a chemotactic protein for polymorphonuclear leukocytes derived from human lung giant cell carcinoma LU65C cells.  J Exp Med  1989  169:1895-901  PubMed ID: 2659722
2856  Yamada T, Hirohashi S, Shimosato Y, Kodama T, Hayashi S, Ogura T, Gamou S, Shimizu N  Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.  Jpn J Cancer Res  1985  76:967-76  PubMed ID: 3935627
5060  Taya Y, Hosogai K, Hirohashi S, Shimosato Y, Tsuchiya R, Tsuchida N, Fushimi M, Sekiya T, Nishimura S.  A novel combination of K-ras and myc amplification accompanied by point mutational activation of K-ras in a human lung cancer.  EMBO J  1984  3(12):2943-6  PubMed ID: 6098458

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User's Publication
11609  Hirata-Nozaki Y, Ohkuri T, Ohara K, Kumai T, Nagata M, Harabuchi S, Kosaka A, Nagato T, Ishibashi K, Oikawa K, Aoki N, Ohara M, Harabuchi Y, Uno Y, Takei H, Celis E, Kobayashi H.  PD-L1-specific helper T-cells exhibit effective antitumor responses: new strategy of cancer immunotherapy targeting PD-L1 in head and neck squamous cell carcinoma.  J Transl Med  2019    PubMed ID: 31221178
10606  Kanno E, Kawasaki O, Takahashi K, Takano K, Endo T.  DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, is a putative tumor suppressor.  Exp. Cell Res.  2018  362:111-120  PubMed ID: 29129563
10922    Loss of IL-24 expression facilitates invasion in lung adenocarcinoma   Int J Clin Exp Pathol  2017  10:173-185 
7462  Tomita T, Ieguchi K, Sawamura T, Maru Y.  Human serum amyloid A3 (SAA3) protein, expressed as a fusion protein with SAA2, binds the oxidized low density lipoprotein receptor.  PLoS ONE  2015  10:e0118835  PubMed ID: 25738827
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309



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