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RCB1963 : VMRC-RCW  update : 2020/03/24
CommentHuman renal cell carcinoma cell line. TKG0447 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Akiyama, M.
Year of deposit 2004
Original cell TKG0447
Animal human < Mammals
Genus Homo
Species sapiens
Tissue kidney
Case history renal cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0447)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 4
User's Publication 8

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3282  Emoto N, Isozaki O, Ohmura E, Ito F, Tsushima T, Shizume K, Demura H, Toma H  Basic fibroblast growth factor (FGF-2) in renal cell carcinoma, which is indistinguishable from that in normal kidney, is involved in renal cell carcinoma growth.  J Urol  1994  152:1626-31  PubMed ID: 7523715
3285  Kitamura M, Shirasawa T, Mitarai T, Muramatsu T, Maruyama N  A retinoid responsive cytokine gene, MK, is preferentially expressed in the proximal tubules of the kidney and human tumor cell lines.  Am J Pathol  1993  142:425-31  PubMed ID: 8434639
3284  Nishimura N, Kanda S, Yogi Y, Kawamura M, Nakamura M, Hyakutake H, Kanetake H, Saito Y  Secretion of autocrine growth-promoting activity by renal-carcinoma cells treated with 5-fluorouracil.  Int J Cancer  1992  52:105-9  PubMed ID: 1500215
3283  Ikemoto S, Hayahara N, Wada S, Nishio S, Maekawa M  Combined effect of tumor necrosis factor alpha and anticancer chemotherapeutic agents against human renal carcinoma cell lines.  Eur Urol  1991  19:236-9  PubMed ID: 1855530

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User's Publication
4371  Isono T, Chano T, Yoshida T, Kageyama S, Kawauchi A, Yonese J, Yuasa T.  Abundance of TRAIL attenuated by HIF2α and c-FLIP affects malignancy in renal cell carcinomas.  Oncotarget  2018  9(33):23091-23101  PubMed ID: 29796174
7050  Kajiura K, Masuda K, Naruto T, Kohmoto T, Watabnabe M, Tsuboi M, Takizawa H, Kondo K, Tangoku A, Imoto I.  Frequent silencing of the candidate tumor suppressor TRIM58 by promoter methylation in early-stage lung adenocarcinoma.  Oncotarget  2016  8:2890-2905  PubMed ID: 27926516
9920  Isono T, Chano T, Yoshida T, Kageyama S, Kawauchi A, Suzaki M, Yuasa T.  Hydroxyl-HIF2-alpha is potential therapeutic target for renal cell carcinomas.  Am J Cancer Res  2016  6:2263-2276  PubMed ID: 27822416
9921  Marichal T, Gaudenzio N, El Abbas S, Sibilano R, Zurek O, Starkl P, Reber LL, Pirottin D, Kim J, Chambon P, Roers A, Antoine N, Kawakami Y, Kawakami T, Bureau F, Tam SY, Tsai M, Galli SJ.  Guanine nucleotide exchange factor RABGEF1 regulates keratinocyte-intrinsic signaling to maintain skin homeostasis.  J. Clin. Invest.  2016  126:4497-4515  PubMed ID: 27820702
6403  Terasaka N, Futai K, Katoh T, Suga H.  A human microRNA precursor binding to folic acid discovered by small RNA transcriptomic SELEX.  RNA  2016  22(12):1918-1928  PubMed ID: 27852928
3744  Nakamura R, Oyama T, Tajiri R, Mizokami A, Namiki M, Nakamoto M, Ooi A.  Expression and regulatory effects on cancer cell behavior of NELL1 and NELL2 in human renal cell carcinoma.  Cancer Sci  2015  106(5):656-64  PubMed ID: 25726761
6384  Hidenori Kawashima Sakae Masaki Marie Kawamura  Induction of HLA‑A* 33‑restricted cytotoxic lymphocytes against renal cell carcinoma targeting galectin 9 and PINCH  Biomedical Reports  2014  2:809-812 
5012  Kawakami T, Chano T, Minami K, Okabe H, Okada Y, Okamoto K.  Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma.  Hum Mol Genet  2006  15(6):821-30  PubMed ID: 16439445

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