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細胞番号 : 細胞名
RCB1955 : KMST-6  update : 2023/09/22
細胞特性(Comment:英)Human cell line derived from embryonic fibroblasts. Characteristics Embryonal fibroblast immortalized by 60-Co irradiation (400 Rad x 1 time and 200 rad x 12 times). Non-tumorigenic. TKG0482 (Deposited from Tohoku Univ.).
細胞特性(日)ヒト胎児線維芽細胞由来細胞株。 TKG0482 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Obinata, Masuo
樹立者 Namba, M.
寄託日 2004
元の細胞 TKG0482
動物種 _human < Mammals
属名 Homo
種名 sapiens
embryo
採取組織 embryonal fibroblast
細胞分類 transformed
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG0482)
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_2998
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
アイソザイム検査 LD, NP
個体識別検査 OK
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 25件
Reference(日) 0件
利用者成果(英) 5件
利用者成果(日) 0件

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Reference(英)
4633  Gao C, Miyazaki M, Kondo T, Tsuji T, Sakaguchi M, Namba M.  Overexpression of platelet-derived growth factor B and downregulation of PDGF-receptor alpha in human immortalized fibroblasts.  Int J Oncol  2001  18(4):871-5  PubMed ID: 11251187   DOI: 10.3892/ijo.18.4.871
4646  Pu H, Sakaguchi M, Kondo T, Kondo A, Kawabata T, Namba M.  Effects of oxygen concentrations on human fibroblasts treated with Fe(3+)-NTA.  Int J Mol Med  2001  7(3):295-300  PubMed ID: 11179510  
4645  Ohashi R, Miyazaki M, Fushimi K, Tsuji T, Inoue Y, Shimizu N, Namba M.  Enhanced activity of cyclin A-associated kinase in immortalized human fibroblasts.  Int J Cancer  1999  82(5):754-8  PubMed ID: 10417776   DOI: 10.1002/(sici)1097-0215(19990827)82:5<754::aid-ijc21>3.0.co;2-p
4644  Fushimi K, Torigoe K, Yamauchi H, Furusako S, Kurimoto M, Namba M.  Establishment of a human fibroblast cell line producing tumor necrosis factor alpha (KMST-6/TNF) and growth inhibitory effects of its conditioned medium on malignant cells in culture.  In Vitro Cell Dev Biol Anim  1998  34(6):463-7  PubMed ID: 9661049   DOI: 10.1007/s11626-998-0079-9
4621  Sugihara S, Mihara K, Marunouchi T, Inoue H, Namba M.  Telomere elongation observed in immortalized human fibroblasts by treatment with 60Co gamma rays or 4-nitroquinoline 1-oxide.  Hum Genet  1996  97(1):1-6  PubMed ID: 8557247   DOI: 10.1007/bf00218823
4643  Jahan I, Iijima M, Kondo T, Namba M.  Effects of okadaic acid on cell growth, anchorage-independent growth, and co-cultures of normal (KMS-6), immortalized (KMST-6), and neoplastically transformed (KMST-6T and KMST-6/RAS) human fibroblasts.  J Cancer Res Clin Oncol  1996  122(4):249-53  PubMed ID: 8601579   DOI: 10.1007/bf01209654
4629  Ogata T, Oshimura M, Namba M, Fujii M, Oishi M, Ayusawa D.  Genetic complementation of the immortal phenotype in group D cell lines by introduction of chromosome 7.  Jpn J Cancer Res  1995  86(1):35-40  PubMed ID: 7737907   DOI: 10.1111/j.1349-7006.1995.tb02985.x
4631  Kondo T, Mihara K, Inoue Y, Iijima M, Namba M.  Two-dimensional electrophoretic analysis of down-regulated proteins in human fibroblasts immortalized by treatment with either 4-nitroquinoline 1-oxide or 60Co gamma rays.  Electrophoresis  1995  16(6):1067-73  PubMed ID: 7498129   DOI: 10.1002/elps.11501601177
4642  Kawashima K1, Jahan I, Fushimi K, Mihara K, Namba M.  Human fibroblasts (KMST-6/RAS cell line) transformed with 60Co gamma-rays and c-Ha-ras oncogene produce a large amount of granulocyte colony-stimulating factor (G-CSF); production is enhanced by cAMP, theophylline, and butyrate.  Cell Struct Funct  1995  20(1):41-5  PubMed ID: 7540958   DOI: 10.1247/csf.20.41
4640  Kawashima K, Mori M, Furusako S, Usuki H, Shimizu N, Namba M.  Granulocyte-colony stimulating factor (G-CSF) production of human fibroblasts (KMST-6/RAS line) transformed with 60Co gamma rays and c-Ha-ras oncogene.  In Vitro Cell Dev Biol Anim  1994  30A(4):199-201  PubMed ID: 7520808   DOI: 10.1007/BF02632040
4641  Kawashima K, Mori M, Furusako S, Usuki H, Namba M.  Human fibroblasts (KMST-6/RAS line) transformed with 60Co gamma-rays and c-Ha-ras oncogene constitutively produce a large amount of human granulocyte-colony stimulating factor (G-CSF).  Hum Cell  1994  7(2):88-94  PubMed ID: 7524638  
4624  Ogata T, Ayusawa D, Namba M, Takahashi E, Oshimura M, Oishi M.  Chromosome 7 suppresses indefinite division of nontumorigenic immortalized human fibroblast cell lines KMST-6 and SUSM-1.  Mol Cell Biol  1993  13(10):6036-43  PubMed ID: 8105370   DOI: 10.1128/mcb.13.10.6036
4628  Namba M, Iijima M, Kondo T, Jahan I, Mihara K.  Immortalization of normal human cells is a multistep process and a rate limiting step of neoplastic transformation of the cells.  Hum Cell  1993  6(4):253-9  PubMed ID: 8148305  
4638  Endo A, Kano Y, Mihara K, Orita K, Namba M.  Alteration in the retinoblastoma gene associated with immortalization of human fibroblasts treated with 60Co gamma rays.  J Cancer Res Clin Oncol  1993  119(9):522-6  PubMed ID: 8100822   DOI: 10.1007/BF01686461
4639  Jahan I, Mihara K, Bai L, Namba M.  Neoplastic transformation and characterization of human fibroblasts by treatment with 60Co gamma rays and the human c-Ha-ras oncogene.  In Vitro Cell Dev Biol Anim  1993  29A(10):763-7  PubMed ID: 8118610   DOI: 10.1007/BF02634342
4627  Bai L, Naomoto Y, Miyazaki M, Orita K, Namba M.  Antiproliferative effects of suramin on human cancer cells in vitro and in vivo.  Acta Med Okayama  1992  46(6):457-63  PubMed ID: 1485540   DOI: 10.18926/AMO/32642
4626  Bai LY, Miyazaki M, Namba M.  Effects of suramin on cell proliferation of various types of human malignant cells  Gan To Kagaku Ryoho  1991  18(3):387-91  PubMed ID: 1848419  
4671  Hatamochi A, Ono M, Ueki H, Namba M.  Regulation of collagen gene expression by transformed human fibroblasts: decreased type I and type III collagen RNA transcription.  J Invest Dermatol  1991  96(4):473-7  PubMed ID: 1706744   DOI: 10.1111/1523-1747.ep12470171
4622  Namba M, Nishitani K, Fukushima F, Kimoto T.  Multistep carcinogenesis of normal human fibroblasts. Human fibroblasts immortalized by repeated treatment with Co-60 gamma rays were transformed into tumorigenic cells with Ha-ras oncogenes.  Anticancer Res  1988  8(5A):947-58  PubMed ID: 3140712  
4637  Namba M, Nishitani K, Fukushima F, Kimoto T, Yuasa Y.  Multi-step neoplastic transformation of normal human fibroblasts by Co-60 gamma rays and Ha-ras oncogenes.  Mutat Res  1988  199(2):415-23  PubMed ID: 3287150   DOI: 10.1016/0027-5107(88)90218-7
4625  Namba M, Karai M, Kimoto T.  Comparison of major cytoskeletons among normal human fibroblasts, immortal human fibroblasts transformed by exposure to Co-60 gamma rays, and the latter cells made tumorigenic by treatment with Harvey murine sarcoma virus.  Exp Gerontol  1987  22(3):179-86  PubMed ID: 3305051   DOI: 10.1016/0531-5565(87)90038-6
4636  Karai M, Namba M.  Interferon-beta can induce the production of plasminogen activator by cultured human cancer cells.  Experientia  1987  43(4):435-7  PubMed ID: 3569495   DOI: 10.1007/bf01940443
4635  Namba M, Nishitani K, Fukushima F, Kimoto T, Nose K.  Multistep process of neoplastic transformation of normal human fibroblasts by 60Co gamma rays and Harvey sarcoma viruses.  Int J Cancer  1986  37(3):419-23  PubMed ID: 3949423   DOI: 10.1002/ijc.2910370314
1025  Namba M, Nishitani K, Hyodoh F, Fukushima F, Kimoto T  Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with 60Co gamma rays.  Int J Cancer  1985  35:275-80  PubMed ID: 3972471   DOI: 10.1002/ijc.2910350221
4634  Namba M.  Neoplastic transformation of human diploid fibroblasts (KMST-6) by treatment with Co-60 gamma rays.  Carcinog Compr Surv  1985  9:217-31  PubMed ID: 4053076  

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利用者成果(英)
16222  Xiaobo Yin, Takayuki Konishi, Kazuo Horikawa, Ryota Tanaka, Yuki Togo, Takanori Noda, Miho Hosoi, Mie Tsuchida, Tatsuki Kunoh, Shuichi Wada, Toshinobu Nakamura, Eisuke Tsuda, Ryuzo Sasaki, Tamio Mizukami, Makoto Hasegawa  Structure and Function of Potential Glycosylation Sites of Dynactin-Associated Protein dynAP  Mol Biotechnol  2022  Jan 13  PubMed ID: 35022995   DOI: 10.1007/s12033-021-00435-3
20153  Yin X, Yamada S, Kobayashi H, Tanaka R, Togo Y, Hosoi M, Tsuchida M, Kunoh T, Wada S, Nakamura T, Sasaki R, Mizukami T, Hasegawa M.  Expression and cell transformation activity of dynactin-associated protein isoforms.  FEBS Open Bio  2021    PubMed ID: 34043884   DOI: 10.1002/2211-5463.13202
4488  Koyama S, Narita E, Shinohara N, Miyakoshi J.  Recovery kinetics of micronucleus formation by fractionated X-ray irradiation in various types of human cells.  J. Radiat. Res  2018  59(5):547-554  PubMed ID: 29961812   DOI: 10.1093/jrr/rry051
5678  Koyama S, Narita E, Shinohara N, Miyakoshi J.  Effect of low-dose X-ray irradiation on micronucleus formation in human embryo, newborn and child cells.  Int J Radiat Biol  2016  92(12):790-795  PubMed ID: 27579525   DOI: 10.1080/09553002.2016.1221544
1744  Gu.,Y., Miyazaki, M., Ohno, T. and Namba M.  Cytotoxicity Test of Ethanol Using Various Human Hepatoma and Other Cell Lines  Alternatives to Animal Testing and Experimentation(AATEX)  1993  2:1-5   

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