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RCB1947 : Ca Ski  update : 2018/08/01
CommentHuman cell line derived from uterine cervical epidermoid carcinoma. TKG0366 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Pattillo, R. A.
Year of deposit 2004
Original cell TKG0366
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 40 years
Tissue cervix
Case history epidermoid carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0366)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 9
User's Publication 5

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3253  Tumor antigen and human chorionic gonadotropin in CaSki cells: a new epidermoid cervical cancer cell line.  Pattillo, R A, Hussa, R O, Story, M T, Ruckert, A C, Shalaby, M R, Mattingly, R F  Science  1977  196:1456-8  PubMed ID: 867042
3255  Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.  Yee C, Krishnan-Hewlett I, Baker, C C, Schlegel R, Howley, P M  Am J Pathol  1985  119:361-6  PubMed ID: 2990217
3254  Human papillomavirus types 16 and 18 sequences in carcinoma cell lines of the cervix.  Pater, M M, Pater A  Virology  1985  145:313-8  PubMed ID: 2992153
3249  Structural and transcriptional analysis of human papillomavirus type 16 sequences in cervical carcinoma cell lines.  Baker, C C, Phelps, W C, Lindgren V, Braun, M J, Gonda, M A, Howley, P M  J Virol  1987  61:962-71  PubMed ID: 3029430
3256  FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines.  Hendricks, D T, Taylor R, Reed M, Birrer, M J  Cancer Res  1997  57:2112-5  PubMed ID: 9187105
3250  [Expression and intracytoplasmic signal transduction pathway of fibroblast growth factor (FGF)-10 in human cervical cancer cell lines]  Kurban G, Ishiwata T, Lu, Y P, Fujii T, Kawahara K, Naito Z, Yamada N, Asano G  J Nippon Med Sch  2001  68:253-8  PubMed ID: 11404772
5053  Optimization of the production of hCG beta-like material by CaSki human cervical carcinoma cells in roller bottle culture.  Hussa RO, Pattillo RA, Ruckert AC, Rinke ML, Cortesi JP, Fein HG.  Oncology  1986  43(1):54-9  PubMed ID: 3753621
5054  A distinctive form of human chorionic gonadotropin beta-subunit-like material produced by cervical carcinoma cells.  Hussa RO, Fein HG, Pattillo RA, Nagelberg SB, Rosen SW, Weintraub BD, Perini F, Ruddon RW, Cole LA.  Cancer Res  1986  46(4 Pt 2):1948-54  PubMed ID: 2418956
5055  Production of tumor-associated antigen, TA-4, by the CaSki cervical carcinoma cell line.  Hussa RO, Maruo T, Strobel JL, Pattillo RA, Mochizuki M.  Obstet Gynecol  1986  67(6):802-5  PubMed ID: 2422615

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User's Publication
7659  HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts to support progression of uterine cervical cancers.  Murata T, Mizushima H, Chinen I, Moribe H, Yagi S, Hoffman RM, Kimura T, Yoshino K, Ueda Y, Enomoto T, Mekada E.  Cancer Res.  2011  71:6633-42  PubMed ID: 22009535
8093  Comparison of fixation methods for preservation of morphology, RNAs, and proteins from paraffin-embedded human cancer cell-implanted mouse models.  Matsuda Y, Fujii T, Suzuki T, Yamahatsu K, Kawahara K, Teduka K, Kawamoto Y, Yamamoto T, Ishiwata T, Naito Z.  J. Histochem. Cytochem.  2010  59:68-75  PubMed ID: 20940453
4371  Abundance of TRAIL attenuated by HIF2α and c-FLIP affects malignancy in renal cell carcinomas.  Isono T, Chano T, Yoshida T, Kageyama S, Kawauchi A, Yonese J, Yuasa T.  Oncotarget  2018  9(33):23091-23101  PubMed ID: 29796174
3516  Analysis of the regulation of fatty acid binding protein 7 expression in human renal carcinoma cell lines.  Takaoka N, Takayama T, Teratani T, Sugiyama T, Mugiya S, Ozono S.  BMC Mol Biol  2011  12:31  PubMed ID: 21771320
6096  Reduction of NOTCH1 expression pertains to maturation abnormalities of keratinocytes in squamous neoplasms.  Sakamoto K, Fujii T, Kawachi H, Miki Y, Omura K, Morita K, Kayamori K, Katsube K, Yamaguchi A.  Lab Invest  2012  92(5):688-702  PubMed ID: 22330335

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