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細胞番号 : 細胞名
RCB1943 : HuH-28  update : 2023/12/25
細胞特性(Comment:英)Human cell line derived from cholangiocellular carcinoma. TKG0438 (Deposited from Tohoku Univ.).
細胞特性(日)ヒト胆管癌由来細胞株。 TKG438 (東北大学医用細胞資源センターからの寄託)。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Obinata, Masuo
樹立者 Kusaka, Y.
寄託日 2004
元の細胞 TKG0438
動物種 _human < Mammals
属名 Homo
種名 sapiens
採取組織 liver
病名 cholangiocellular carcinoma
細胞分類 cancer
入手歴 Cell Resource Center for Biomedical Research, Tohoku University(TKG00438)
細胞寿命 infinite
細胞形態 other
Cellosaurus(Expasy) CVCL_2955
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 MEM + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin + (0.02% EDTA or 0.04% EDTA)
継代密度 1 : 4 split
継代・培地交換頻度 Subculture : once/2 weeks, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HBV) (-)
ウィルス(HCV) (-)
アイソザイム検査 LD, NP
個体識別検査 OK
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 25件
利用者成果(日) 0件

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Reference(英)
5051  Kusaka Y1, Muraoka A, Tokiwa T, Sato J.  Establishment and characterization of a human cholangiocellular carcinoma cell line  Hum Cell  1988  1(1):92-4  PubMed ID: 2856443  
5052  Kusaka Y1, Tokiwa T, Sato J.  Establishment and characterization of a cell line from a human cholangiocellular carcinoma.  Res Exp Med (Berl)  1988  188(5):367-75  PubMed ID: 2852388   DOI: 10.1007/bf01851205

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21553  Louis C, Ferlier T, Leroux R, Pineau R, Desoteux M, Papoutsoglou P, Leclerc D, Angenard G, Vaquero J, Macias RIR, Edeline J, Coulouarn C.  TGFβ-induced circLTBP2 predicts a poor prognosis in intrahepatic cholangiocarcinoma and mediates gemcitabine resistance by sponging miR-338-3p.  JHEP Rep  2023  5(12):100900  PubMed ID: 38023605   DOI: 10.1016/j.jhepr.2023.100900
17715  Zhang L, Ma D, Li F, Qiu G, Sun D, Zeng Z.  Lnc-PKD2-2-3/miR-328/GPAM ceRNA Network Induces Cholangiocarcinoma Proliferation, Invasion and 5-FU Chemoresistance  Front Oncol  2022  12:871281  PubMed ID: 35965521   DOI: 10.3389/fonc.2022.871281
21148  Kosai-Fujimoto Y, Itoh S, Yugawa K, Fukuhara T, Okuzaki D, Toshima T, Harada N, Oda Y, Yoshizumi T, Mori M.  Impact of JMJD6 on intrahepatic cholangiocarcinoma.  Mol Clin Oncol  2022  17(2):131  PubMed ID: 35911665   DOI: 10.3892/mco.2022.2564
15892  Khorsandi SE, Dokal AD, Rajeeve V, Britton DJ, Illingworth MS, Heaton N, Cutillas PR.  Computational Analysis of Cholangiocarcinoma Phosphoproteomes Identifies Patient-Specific Drug Targets.  Cancer Res  2021  81(22):5765-5776  PubMed ID: 34551960   DOI: 10.1158/0008-5472.CAN-21-0955
12589  Miyata T, Yamashita YI, Yoshizumi T, Shiraishi M, Ohta M, Eguchi S, Aishima S, Fujioka H, Baba H.  CXCL12 expression in intrahepatic cholangiocarcinoma is associated with metastasis and poor prognosis  Cancer Sci   2019  110(10):3197-3203  PubMed ID: 31361379   DOI: 10.1111/cas.14151
14816  Goyal L, Shi L, Liu LY, Fece de la Cruz F, Lennerz JK, Raghavan S, Leschiner I, Elagina L, Siravegna G, Ng RWS, Vu P, Patra KC, Saha SK, Uppot RN, Arellano R, Reyes S, Sagara T, Otsuki S, Nadres B, Shahzade HA, Dey-Guha I, Fetter IJ, Baiev I, Van Seventer EE, Murphy JE, Ferrone CR, Tanabe KK, Deshpande V, Harding JJ, Yaeger R, Kelley RK, Bardelli A, Iafrate AJ, Hahn WC, Benes CH, Ting DT, Hirai H, Getz G, Juric D, Zhu AX, Corcoran RB, Bardeesy N.  TAS-120 Overcomes Resistance to ATP-Competitive FGFR Inhibitors in Patients with FGFR2 Fusion-Positive Intrahepatic Cholangiocarcinoma  Cancer Discov  2019  9(8):1064-1079  PubMed ID: 31109923   DOI: 10.1158/2159-8290.CD-19-0182
10740  Merdrignac A, Angenard G, Allain C, Petitjean K, Bergeat D, Bellaud P, Fautrel A, Turlin B, Clément B, Dooley S, Sulpice L, Boudjema K, Coulouarn C.  A novel transforming growth factor beta-induced long noncoding RNA promotes an inflammatory microenvironment in human intrahepatic cholangiocarcinoma.  Hepatol Commun  2018  2:254-269  PubMed ID: 29507901   DOI: 10.1002/hep4.1142
10770  Mao Y, Huang X, Shuang Z, Lin G, Wang J, Duan F, Chen J, Li S.  PARP inhibitor olaparib sensitizes cholangiocarcinoma cells to radiation.  Cancer Med  2018  7:1285-1296  PubMed ID: 29479816   DOI: 10.1002/cam4.1318
4487  Shuang Z, Mao Y, Lin G, Wang J, Huang X, Chen J, Duan F, Li S.  Alpha-L-Fucosidase Serves as a Prognostic Indicator for Intrahepatic Cholangiocarcinoma and Inhibits Its Invasion Capacity.  iomed Res Int  2018  2018:8182575  PubMed ID: 29682557   DOI: 10.1155/2018/8182575
10055  Tan X, Huang Z, Li X.  Long Non-Coding RNA MALAT1 Interacts With miR-204 to Modulate Human Hilar Cholangiocarcinoma Proliferation, Migration, and Invasion by Targeting CXCR4.  J. Cell. Biochem.  2017  118:3643-3653  PubMed ID: 28059437   DOI: 10.1002/jcb.25862
10501  Kushibiki T, Noji T, Ebihara Y, Hontani K, Ono M, Kuwabara S, Nakamura T, Tsuchikawa T, Okamura K, Ishizuka M, Hirano S.  5-Aminolevulinic-acid-mediated Photodynamic Diagnosis Enhances the Detection of Peritoneal Metastases in Biliary Tract Cancer in Mice.  In Vivo  2017  31:905-908  PubMed ID: 28882957   DOI: 10.21873/invivo.11145
14252  Maki T, Ikeda H, Kuroda A, Kyogoku N, Yamamura Y, Tabata Y, Abiko T, Tsuchikawa T, Hida Y, Shichinohe T, Tanaka E, Kaga K, Hatanaka K, Matsuno Y, Imai N, Hirano S.  Differential detection of cytoplasmic Wilms tumor 1 expression by immunohistochemistry, western blotting and mRNA quantification  Int J Oncol  2017  50(1):129-140.  PubMed ID: 27922671   DOI: 10.3892/ijo.2016.3786
14271  Muguruma N, Okamoto K, Nakagawa T, Sannomiya K, Fujimoto S, Mitsui Y, Kimura T, Miyamoto H, Higashijima J, Shimada M, Horino Y, Matsumoto S, Hanaoka K, Nagano T, Shibutani M, Takayama T.  Molecular imaging of aberrant crypt foci in the human colon targeting glutathione S-transferase P1-1  Sci Rep  2017  7(1):6536  PubMed ID: 28747791   DOI: 10.1038/s41598-017-06857-x
14528  Yagai T, Matsui S, Harada K, Inagaki FF, Saijou E, Miura Y, Nakanuma Y, Miyajima A, Tanaka M.  Expression and localization of sterile alpha motif domain containing 5 is associated with cell type and malignancy of biliary tree  PLoS One  2017  12(4):e0175355  PubMed ID: 28388653   DOI: 10.1371/journal.pone.0175355
12673  Cavalloni G, Peraldo-Neia C, Varamo C, Casorzo L, Dell'Aglio C, Bernabei P, Chiorino G, Aglietta M, Leone F.  Establishment and characterization of a human intrahepatic cholangiocarcinoma cell line derived from an Italian patient  Tumour Biol   2016  37(3):4041-52  PubMed ID: 26486326   DOI: 10.1007/s13277-015-4215-3
12878  Yang SH, Lin HY, Changou CA, Chen CH, Liu YR, Wang J, Jiang X, Luh F, Yen Y.  Integrin β3 and LKB1 are independently involved in the inhibition of proliferation by lovastatin in human intrahepatic cholangiocarcinoma  Oncotarget  2016  7(1):362-73  PubMed ID: 26517522   DOI: 10.18632/oncotarget.6238
14116  Huyen NT, Prachayasittikul V, Chan-On W.  Anoikis-resistant cholangiocarcinoma cells display aggressive characteristics and increase STAT3 activation  J Hepatobiliary Pancreat Sci  2016  23(7):397-405  PubMed ID: 27107220   DOI: 10.1002/jhbp.354
9170  Sheng-Huei Yang 1, Hung-Yun Lin, Vincent H S Chang, Chien-Chung Chen, Yun-Ru Liu, Jinghan Wang, Keqiang Zhang, Xiaoqing Jiang, Yun Yen  Lovastatin Overcomes Gefitinib Resistance Through TNF-α Signaling in Human Cholangiocarcinomas With Different LKB1 Statuses in Vitro and in Vivo  Oncotarget  2015  6 (27), 23857-73  PubMed ID: 26160843   DOI: 10.18632/oncotarget.4408
14311  Chan-On W, Huyen NT, Songtawee N, Suwanjang W, Prachayasittikul S, Prachayasittikul V.  Quinoline-based clioquinol and nitroxoline exhibit anticancer activity inducing FoxM1 inhibition in cholangiocarcinoma cells  Drug Des Devel Ther  2015  9:2033-47  PubMed ID: 25897210   DOI: 10.2147/DDDT.S79313
15203  Wang J, Zhang K, Wang J, Wu X, Liu X, Li B, Zhu Y, Yu Y, Cheng Q, Hu Z, Guo C, Hu S, Mu B, Tsai CH, Li J, Smith L, Yang L, Liu Q, Chu P, Chang V, Zhang B, Wu M, Jiang X, Yen Y.  Underexpression of LKB1 tumor suppressor is associated with enhanced Wnt signaling and malignant characteristics of human intrahepatic cholangiocarcinoma  Oncotarget  2015  6(22):18905-20  PubMed ID: 26056085   DOI: 10.18632/oncotarget.4305
14930  Takahashi N, Aoyama F, Hiyoshi M, Kataoka H, Sawaguchi A.  Establishment and biological characterization of a novel cell line derived from hepatoid adenocarcinoma originated at the ampulla of Vater  Int J Oncol  2014  44(4):1139-45  PubMed ID: 24481592   DOI: 10.3892/ijo.2014.2282
3722  Ohashi H, Adachi Y, Yamamoto H, Taniguchi H, Nosho K, Suzuki H, Arimura Y, Imai K, Carbone DP, Shinomura Y.  Insulin-like growth factor receptor expression is associated with aggressive phenotypes and has therapeutic activity in biliary tract cancers.  Cancer Sci  2012  103(2):252-61  PubMed ID: 22044563   DOI: 10.1111/j.1349-7006.2011.02138.x
7938  Sugiyama H, Onuki K, Ishige K, Baba N, Ueda T, Matsuda S, Takeuchi K, Onodera M, Nakanuma Y, Yamato M, Yamamoto M, Hyodo I, Shoda J.  Potent in vitro and in vivo antitumor activity of sorafenib against human intrahepatic cholangiocarcinoma cells.  J. Gastroenterol.  2011  46:779-89  PubMed ID: 21331764   DOI: 10.1007/s00535-011-0380-3
15724  Araki K, Shimura T, Suzuki H, Tsutsumi S, Wada W, Yajima T, Kobayahi T, Kubo N, Kuwano H.  E/N-cadherin switch mediates cancer progression via TGF-β-induced epithelial-to-mesenchymal transition in extrahepatic cholangiocarcinoma  Br J Cancer  2011  105(12):1885-93  PubMed ID: 22068819   DOI: 10.1038/bjc.2011.452
18737  Kawase H, Fujii K, Miyamoto M, Kubota KC, Hirano S, Kondo S, Inagaki F.  Differential LC-MS-based proteomics of surgical human cholangiocarcinoma tissues  J Proteome Res  2009  8(8):4092-103  PubMed ID: 19569727   DOI: 10.1021/pr900468k

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