Cell No. : Cell Name RCB1934 : HUT78  update : 2024/01/17
Comment			Human cell line derived from T cell of a patient with Sezary syndrome. Producing IL-2. TKG0375 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Year of deposit	2004
		Original cell	TKG0375
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	Peripheral blood
		Disease name	Peripheral blood of a patient with Sezary syndrome
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0375）
		Lifespan	infinite
		Morphology	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_0337
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		RPMI1640 + 10% FBS
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		STR(human)		OK
Reference information		Reference	2
		User's Publication	2

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Reference

5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898

5046  Gootenberg JE, Ruscetti FW, Mier JW, Gazdar A, Gallo RC.  Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor.  J Exp Med  1981  154(5):1403-18  PubMed ID: 6975346   DOI: 10.1084/jem.154.5.1403

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User's Publication

13384  Tsuji S, Kohyanagi N, Mizuno T, Ohama T, Sato K.  Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.  Oncol Lett  2021    PubMed ID: 33376545   DOI: 10.3892/ol.2020.12374

15877  Kitagawa K, Shibata K, Matsumoto A, Matsumoto M, Ohhata T, Nakayama KI, Niida H, Kitagawa M.  Fbw7 targets GATA3 through cyclin-dependent kinase 2-dependent proteolysis and contributes to regulation of T-cell development  Mol Cell Biol  2014  34(14):2732-44  PubMed ID: 24820417   DOI: 10.1128/MCB.01549-13