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RCB1932 : T2  update : 2018/12/04
CommentHuman hybrid (T and B lymphoblast) cell line. Fusion cll line between 174 and CEM.T2. This hybrid cells do not express MHC class II. HLA-A2 positive. TKG0599 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0599
Animal human x human < Hybrid
Genus Homo
Species sapiens
Tissue hybrid cell
Case history 174 x CEM.T2
Classification hybrid cells
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0599)
Lifespan infinite
Morphology lymphocyte-like
hybridoma Fusion_cell_1 name LCL721 B cell
Fusion_cell_1 strain human
Fusion_cell_2 name CEM.T cell
Fusion_cell_2 strain human
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 81-90(50) : /84(6),85(7),86(8),87(6),88(8),89(5)/
Images
deposit info
lot info
Reference information Reference 2
User's Publication 1


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Reference
3274  Salter, R D, Howell, D N, Cresswell P  Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids.  Immunogenetics  1985  21:235-46  PubMed ID: 3872841
3281  Kavathas P, Bach, F H, DeMars R  Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.  Proc Natl Acad Sci U S A  1980  77:4251-5  PubMed ID: 6933474

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User's Publication
7984  Tomita Y, Imai K, Senju S, Irie A, Inoue M, Hayashida Y, Shiraishi K, Mori T, Daigo Y, Tsunoda T, Ito T, Nomori H, Nakamura Y, Kohrogi H, Nishimura Y.  A novel tumor-associated antigen, cell division cycle 45-like can induce cytotoxic T-lymphocytes reactive to tumor cells.  Cancer Sci.  2011  102:697-705  PubMed ID: 21231984



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