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RCB1900 : Lu99  update : 2018/08/01
CommentLung giant cell carcinoma cell line derived from human. TKG0495 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Hirohashi, S. and Shimosatao, Y.
Year of deposit 2004
Original cell TKG0495
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 63 years
Tissue lung
Case history Giant cell carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0495)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 53-58(50) : 53(5),54(9),55(12),56(12),57(6),58(6)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 5


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Reference
2856  Yamada T, Hirohashi S, Shimosato Y, Kodama T, Hayashi S, Ogura T, Gamou S, Shimizu N  Giant cell carcinomas of the lung producing colony-stimulating factor in vitro and in vivo.  Jpn J Cancer Res  1985  76:967-76  PubMed ID: 3935627

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User's Publication
11496  Miyake N, Chikumi H, Yamaguchi K, Takata M, Takata M, Okada K, Kitaura T, Nakamoto M, Yamasaki A.  Effect of Cetuximab and EGFR Small Interfering RNA Combination Treatment in NSCLC Cell Lines with Wild Type EGFR and Use of KRAS as a Possible Biomarker for Treatment Responsiveness.  Yonago Acta Med  2019    PubMed ID: 30962749
4280  Rawangkan A, Wongsirisin P, Namiki K, Iida K, Kobayashi Y, Shimizu Y, Fujiki H, Suganuma M.  Green Tea Catechin Is an Alternative Immune Checkpoint Inhibitor that Inhibits PD-L1 Expression and Lung Tumor Growth.  Molecules  2018    PubMed ID: 30126206
7006  Iida K, Sakai R, Yokoyama S, Kobayashi N, Togo S, Yoshikawa HY, Rawangkan A, Namiki K, Suganuma M.  Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL.  Sci Rep  2017  7:17770  PubMed ID: 29259259
7462  Tomita T, Ieguchi K, Sawamura T, Maru Y.  Human serum amyloid A3 (SAA3) protein, expressed as a fusion protein with SAA2, binds the oxidized low density lipoprotein receptor.  PLoS ONE  2015  10:e0118835  PubMed ID: 25738827
5833  Sato M, Muguruma N, Nakagawa T, Okamoto K, Kimura T, Kitamura S, Yano H, Sannomiya K, Goji T, Miyamoto H, Okahisa T, Mikasa H, Wada S, Iwata M, Takayama T.  High antitumor activity of pladienolide B and its derivative in gastric cancer.  Cancer Sci  2014  105(1):110-6  PubMed ID: 24635824



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