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RCB1890 : MG-63  update : 2018/08/01
CommentOsteosarcoma cell line derived from human. High yield of IFN production by induction with poly IC, cyclohexmide, actinomycin D. Antigenically, MG-63 IFN is closely related to human fibroblast IFN. TKG0294 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0294
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 years
Tissue bone
Case history Osteosarcoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0294)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Isozyme LD, NP
Chromosome mode 105-115(50) : /107(8),108(4),109(12),110(7),111(6)/
deposit info
lot info
Reference information Reference 2
User's Publication 18

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2851  Human interferon: mass production in a newly established cell line, MG-63.  Billiau A, Edy VG, Heremans H, Van Damme J, Desmyter J, Georgiades JA, De Somer P  Antimicrob Agents Chemother  1977  12:11-5  PubMed ID: 883813
4998  In vitro cultivation of human tumor tissues. II. Morphological and virological characterization of three cell lines.  Heremans H, Billiau A, Cassiman JJ, Mulier JC, de Somer P.  Oncology  1978  35(6):246-52  PubMed ID: 218153

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User's Publication
7675  Thrombin induces osteosarcoma growth, a function inhibited by low molecular weight heparin in vitro and in vivo: procoagulant nature of osteosarcoma.  Ichikawa J, Cole HA, Magnussen RA, Mignemi NA, Butler M, Holt GE, O'Rear L, Yuasa M, Pabla B, Haro H, Cates JM, Hamm HE, Schwartz HS, Schoenecker JG.  Cancer  2011  118:2494-506  PubMed ID: 21953059
7896  Specific interaction between Ca2+ and anionic lipids in cancer cells along with apoptosis.  Goto K, Tatsumi K, Nagata T, Komizu Y, Ueoka R.  Biol. Pharm. Bull.  2011  34:439-42  PubMed ID: 21372400
8031  Ameloblastin regulates osteogenic differentiation by inhibiting Src kinase via cross talk between integrin beta1 and CD63.  Iizuka S, Kudo Y, Yoshida M, Tsunematsu T, Yoshiko Y, Uchida T, Ogawa I, Miyauchi M, Takata T.  Mol. Cell. Biol.  2010  31:783-92  PubMed ID: 21149578
8073  Silencing of autocrine motility factor induces mesenchymal-to-epithelial transition and suppression of osteosarcoma pulmonary metastasis.  Niinaka Y, Harada K, Fujimuro M, Oda M, Haga A, Hosoki M, Uzawa N, Arai N, Yamaguchi S, Yamashiro M, Raz A.  Cancer Res.  2010  70:9483-93  PubMed ID: 20978190
8446  Involvement of ETS1 in thioredoxin-binding protein 2 transcription induced by a synthetic retinoid CD437 in human osteosarcoma cells.  Hashiguchi K, Tsuchiya H, Tomita A, Ueda C, Akechi Y, Sakabe T, Kurimasa A, Nozaki M, Yamada T, Tsuchida S, Shiota G.  Biochem. Biophys. Res. Commun.  2009  391:621-6  PubMed ID: 19932085
9956  AICAR induces mitochondrial apoptosis in human osteosarcoma cells through an AMPK-dependent pathway.  Morishita M, Kawamoto T, Hara H, Onishi Y, Ueha T, Minoda M, Katayama E, Takemori T, Fukase N, Kurosaka M, Kuroda R, Akisue T.  Int. J. Oncol.  2017  50:23-30  PubMed ID: 27878239
10070  Fluid shear stress applied by orbital shaking induces MG-63 osteosarcoma cells to activate ERK in two phases through distinct signaling pathways.  Fukada T, Sakajiri H, Kuroda M, Kioka N, Sugimoto K.  Biochem Biophys Rep  2017  9:257-265  PubMed ID: 28956013
10571  Synergistic Effects of a Smac Mimetic with Doxorubicin Against Human Osteosarcoma.  Kamata E, Kawamoto T, Ueha T, Hara H, Fukase N, Minoda M, Morishita M, Takemori T, Fujiwara S, Nishida K, Kuroda R, Kurosaka M, Akisue T.  Anticancer Res.  2017  37:6097-6106  PubMed ID: 29061790
10809  Interaction between human osteosarcoma and mesenchymal stem cells via an interleukin-8 signaling loop in the tumor microenvironment.  Kawano M, Tanaka K, Itonaga I, Iwasaki T, Tsumura H.  Cell Commun. Signal  2018  16:13  PubMed ID: 29625612
10906  The effect of macro/micro combination pore structure of biphasic calcium phosphate scaffold on bioactivity    Ceramics International  2017  43, 3540-3546 
10919  Antitumor activities of BIBF 1120, BI 860585, and BI 836845 in preclinical models of sarcoma    Integr Mol Med  2016  3: 755-760 
11414  Chloroquine Enhances Rapamycin-induced Apoptosis in MG63 Cells.  Ishibashi Y, Nakamura O, Yamagami Y, Nishimura H, Fukuoka N, Yamamoto T.  Anticancer Res.  2019    PubMed ID: 30711941
4056  tLyP-1-conjugated Au-nanorod@SiO(2) core-shell nanoparticles for tumor-targeted drug delivery and photothermal therapy.  Xu B, Ju Y, Cui Y, Song G, Iwase Y, Hosoi A, Morita Y.  Langmuir  2014  30:7789-97  PubMed ID: 24921672
4424  Synergistic effect of IL­2, IL­12 and IL­18 on the activation of ex vivo­expanded human Vγ9Vδ2 T cells  Eisuke DOMAE, Kaname TSUJI, Yuya HIRAI, Yoshihiro YOSHIKAWA, Aiko KAMADA, Shosuke MORITA, Seiji GODA, Takashi IKEO  J Osaka Dent Univ  2018  52 : 51−57 
4291  The effect of hydroxyapatite nanoparticles on mechanical behavior and biological performance of porous shape memory polyurethane scaffolds.  Yu J, Xia H, Teramoto A, Ni QQ.  J Biomed Mater Res A  2018    PubMed ID: 28880433
4292  Platelet-Derived TGF-β Induces Tissue Factor Expression via the Smad3 Pathway in Osteosarcoma Cells.  Saito M, Ichikawa J, Ando T, Schoenecker JG, Ohba T, Koyama K, Suzuki-Inoue K, Haro H.  J. Bone Miner. Res.  2018    PubMed ID: 29949655
11668  Collagen production ability of milk basic protein is dependent on stimulatory effect of transforming growth factor-β1 and β2  Aiko Ono-Ohmachi, Hiroshi M. Ueno, Yoshikazu Morita, Ken Kato  International Dairy Journal  2019   
6661  Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence.  Kamiyama F, Maeda T, Yamane T, Li YH, Ogukubo O, Otsuka T, Ueyama H, Takahashi S, Ohkubo I, Matsui N.  Biochem Biophys Res Commun  2001  288(4):975-80  PubMed ID: 11689005

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