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RCB1886 : Hep G2  update : 2018/08/01
CommentHuman cell line derived from hepatocyte carcinoma. TKG0205 (Deposited from Tohoku Univ.). Information
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Year of deposit 2004
Original cell TKG0205 HepG2
Animal human < Mammals
Genus Homo
Species sapiens
Race Caucasian
Gender Male
Age at sampling 15 years
Tissue liver
Case history hepatocyte carcinoma
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0205)
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR (human) OK
Virus (HBV) (-)
Virus (HCV) (-)
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 71


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Reference

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User's Publication
7067  Yamada K, Mito F, Matsuoka Y, Ide S, Shikimachi K, Fujiki A, Kusakabe D, Ishida Y, Enoki M, Tada A, Ariyoshi M, Yamasaki T, Yamato M.  Fluorescence probes to detect lipid-derived radicals.  Nat. Chem. Biol.  2016  12:608-13  PubMed ID: 27294322
7092  M Khanmohammadi, S Sakai, T Ashida, M Taya  Production of hyaluronic-acid-based cell-enclosing microparticles and microcapsules via enzymatic reaction using a microfluidic system.  J Applied Polymer Science  2016  133:43107 
7125  Darwish WS, Ikenaka Y, Nakayama SM, Mizukawa H, Ishizuka M.  Constitutive Effects of Lead on Aryl Hydrocarbon Receptor Gene Battery and Protection by β-carotene and Ascorbic Acid in Human HepG2 Cells.  J. Food Sci.  2015  81:T275-81  PubMed ID: 26630500
7128  Sato Y, Sasaki T, Takahashi S, Kumagai T, Nagata K.  Development of a highly reproducible system to evaluate inhibition of cytochrome P450 3A4 activity by natural medicines.  J Pharm Pharm Sci  2015  18:316-27  PubMed ID: 26626238
7153  Takahashi S, Morita K, Kinoshita M, Fujimori S, Ishikawa T.  Effects of scorched food leachates with or without activated charcoal pretreatment on AhR activation in cultured cells.  J Toxicol Sci  2015  40:777-86  PubMed ID: 26558458
7219  Yamaza T, Alatas FS, Yuniartha R, Yamaza H, Fujiyoshi JK, Yanagi Y, Yoshimaru K, Hayashida M, Matsuura T, Aijima R, Ihara K, Ohga S, Shi S, Nonaka K, Taguchi T.  In vivo hepatogenic capacity and therapeutic potential of stem cells from human exfoliated deciduous teeth in liver fibrosis in mice.  Stem Cell Res Ther  2015  6:171  PubMed ID: 26358689
7238  Ozeki M, Salah A, Aini W, Tamaki K, Haga H, Miyagawa-Hayashino A.  Abnormal Localization of STK17A in Bile Canaliculi in Liver Allografts: An Early Sign of Chronic Rejection.  PLoS ONE  2015  10:e0136381  PubMed ID: 26305096
7257  Anan Y, Kimura M, Hayashi M, Koike R, Ogra Y.  Detoxification of selenite to form selenocyanate in mammalian cells.  Chem. Res. Toxicol.  2015  28:1803-14  PubMed ID: 26243445
7270  Takei T, Kitazono Z, Ozuno Y, Yoshinaga T, Nishimata H, Yoshida M.  Vascular-like network prepared using hollow hydrogel microfibers.  J. Biosci. Bioeng.  2015  121:336-40  PubMed ID: 26199226
7278  Yamaori S, Kinugasa Y, Jiang R, Takeda S, Yamamoto I, Watanabe K.  Cannabidiol induces expression of human cytochrome P450 1A1 that is possibly mediated through aryl hydrocarbon receptor signaling in HepG2 cells.  Life Sci.  2015  136:87-93  PubMed ID: 26187180
7324  Somiya M, Yamaguchi K, Liu Q, Niimi T, Maturana AD, Iijima M, Yoshimoto N, Kuroda S.  One-step scalable preparation method for non-cationic liposomes with high siRNA content.  Int J Pharm  2015  490:316-23  PubMed ID: 26027493
7373  Takei T, Aokawa R, Shigemitsu T, Kawakami K, Yoshida M.  Fabrication of uniformly cell-laden porous scaffolds using a gas-in-liquid templating technique.  J. Biosci. Bioeng.  2015  120:577-81  PubMed ID: 25912452
7474  Suzuki Y, Nakagawa K, Kato S, Tatewaki N, Mizuochi S, Ito J, Eitsuka T, Nishida H, Miyazawa T.  Metabolism and cytotoxic effects of phosphatidylcholine hydroperoxide in human hepatoma HepG2 cells.  Biochem. Biophys. Res. Commun.  2015  458:920-7  PubMed ID: 25704087
7476  Yanagida A, Nakauchi H, Kamiya A.  Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells.  Methods Mol. Biol.  2015  1357:295-310  PubMed ID: 25697415
7553  Priscilla Boccia, Claudia Meconi, Elena Sturchio, Salvatore Margiotta, Pietro Ragone, Vito Summa  Natural geochemical risk in the Pollino Massif: a case-study of chromium.  Frontiers in Genetics  2015  6: 
7568  Tung NH, Uto T, Morinaga O, Shoyama Y  Chemical Constituents from the Aerial Parts of Bupleurum falcatum L. and Biological Evidences.  Natural Product Sciences  2015  21:71-75 
9536  Kumagai T, Aratsu Y, Sugawara R, Sasaki T, Miyairi S, Nagata K.  Indirubin, a component of Ban-Lan-Gen, activates CYP3A4 gene transcription through the human pregnane X receptor.  Drug Metab. Pharmacokinet.  2016  31:139-45  PubMed ID: 26987505
9555  Watanabe S, Moniaga CS, Nielsen S, Hara-Chikuma M.  Aquaporin-9 facilitates membrane transport of hydrogen peroxide in mammalian cells.  Biochem. Biophys. Res. Commun.  2016  471:191-7  PubMed ID: 26837049
9642  Darwish AG, Samy MN, Sugimoto S, Otsuka H, Abdel-Salam H, Matsunami K.  Effects of Hepatoprotective Compounds from the Leaves of Lumnitzera racemosa on Acetaminophen-Induced Liver Damage in Vitro.  Chem. Pharm. Bull.  2016  64:360-5  PubMed ID: 27039833
9701  Zaitsu Y, Iwatake M, Sato K, Tsukuba T.  Lipid droplets affect elimination of Porphyromonas gingivalis in HepG2 cells by altering the autophagy-lysosome system.  Microbes Infect.  2016  18:565-71  PubMed ID: 27237960
9717  Miyamae Y, Nishito Y, Nakai N, Nagumo Y, Usui T, Masuda S, Kambe T, Nagao M.  Tetrandrine induces lipid accumulation through blockade of autophagy in a hepatic stellate cell line.  Biochem. Biophys. Res. Commun.  2016  477:40-6  PubMed ID: 27270032
9775  Khoshfetrat AB, Khanmohammadi M, Sakai S, Taya M.  Enzymatically-gellable galactosylated chitosan: Hydrogel characteristics and hepatic cell behavior.  Int. J. Biol. Macromol.  2016  92:892-899  PubMed ID: 27496605
9812  Lin Y, Ohkawara B, Ito M, Misawa N, Miyamoto K, Takegami Y, Masuda A, Toyokuni S, Ohno K.  Molecular hydrogen suppresses activated Wnt/β-catenin signaling.  Sci Rep  2016  6:31986  PubMed ID: 27558955
9841  Burdeos GC, Ito J, Eitsuka T, Nakagawa K, Kimura F, Miyazawa T.  δ and γ tocotrienols suppress human hepatocellular carcinoma cell proliferation via regulation of Ras-Raf-MEK-ERK pathway-associated upstream signaling.  Food Funct  2016  7:4170-4174  PubMed ID: 27713963
9946  Abiko Y, Lin FY, Lee H, Puga A, Kumagai Y.  Quinone-mediated induction of cytochrome P450 1A1 in HepG2 cells through increased interaction of aryl hydrocarbon receptor with aryl hydrocarbon receptor nuclear translocator.  J Toxicol Sci  2016  41:775-781  PubMed ID: 27853106
9988  Li S, Gao A, Dong S, Chen Y, Sun S, Lei Z, Zhang Z.  Purification, antitumor and immunomodulatory activity of polysaccharides from soybean residue fermented with Morchella esculenta.  Int. J. Biol. Macromol.  2017  96:26-34  PubMed ID: 27939510
9990  Tanaka Y, Tateishi K, Nakatsuka T, Kudo Y, Takahashi R, Miyabayashi K, Yamamoto K, Asaoka Y, Ijichi H, Tateishi R, Shibahara J, Fukayama M, Ishizawa T, Hasegawa K, Kokudo N, Koike K.  Sharpin promotes hepatocellular carcinoma progression via transactivation of Versican expression.  Oncogenesis  2016  5:e277  PubMed ID: 27941932
9996  Arnal JF, Battle T, Rasetti C, Challah M, Costerousse O, Vicaut E, Michel JB, Alhenc-Gelas F.  ACE in three tunicae of rat aorta: expression in smooth muscle and effect of renovascular hypertension.  Am. J. Physiol.  1994  267:H1777-84  PubMed ID: 7977808
10002  Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Ishige N.  Cell death in a co-culture of hepatocellular carcinoma cells and human umbilical vascular endothelial cells in a medium lacking glucose and arginine.  Oncol Lett  2017  13:258-262  PubMed ID: 28123551
10080  Wakuri S, Yamakage K, Kazuki Y, Kazuki K, Oshimura M, Aburatani S, Yasunaga M, Nakajima Y.  Correlation between luminescence intensity and cytotoxicity in cell-based cytotoxicity assay using luciferase.  Anal. Biochem.  2017  522:18-29  PubMed ID: 28111305
10167  Takahashi K, Suzuki N, Ogra Y.  Bioavailability Comparison of Nine Bioselenocompounds In Vitro and In Vivo.  Int J Mol Sci  2017  18:  PubMed ID: 28245633
10236  Sudo K, Niikura K, Iwaki K, Kohyama S, Fujiwara K, Doi N.  Human-derived fusogenic peptides for the intracellular delivery of proteins.  J Control Release  2017  255:1-11  PubMed ID: 28385674
10351  Nakashima KI, Ogiwara T, Hirai T, Tanaka T, Murata H, Kaburagi K, Fujii-Kuriyama Y, Hayashi H, Inoue M.  Gerontoxanthone B from Maclura cochinchinensis var. gerontogea exhibits anti-inflammatory potential as an aryl hydrocarbon receptor agonist.  Bioorg. Med. Chem.  2017  25:4253-4258  PubMed ID: 28662965
10392  Ono Y, Tomimori N, Hori H, Kitagawa Y, Shibata H.  Mechanisms of chromosomal aberrations induced by sesamin metabolites in Chinese hamster lung cells.  Mutat. Res.  2017  822:19-26  PubMed ID: 28844238
10435  Tung NH, Uto T, Hai NT, Li G, Shoyama Y.  Quassinoids from the Root of Eurycoma longifolia and Their Antiproliferative Activity on Human Cancer Cell Lines.  Pharmacogn Mag  2017  13:459-462  PubMed ID: 28839372
10446  Chen Z, Wu Y, Ma YS, Kobayashi Y, Zhao YY, Miura Y, Chiba H, Hui SP.  Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MS.  Anal Bioanal Chem  2017  409:5735-5745  PubMed ID: 28762068
10466  Machihara K, Tanaka H, Hayashi Y, Murakami I, Namba T.  Questiomycin A stimulates sorafenib-induced cell death via suppression of glucose-regulated protein 78.  Biochem. Biophys. Res. Commun.  2017  492:33-40  PubMed ID: 28811106
10470  Xu Z, Huo J, Ding X, Yang M, Li L, Dai J, Hosoe K, Kubo H, Mori M, Higuchi K, Sawashita J.  Coenzyme Q10 Improves Lipid Metabolism and Ameliorates Obesity by Regulating CaMKII-Mediated PDE4 Inhibition.  Sci Rep  2017  7:8253  PubMed ID: 28811612
10478  Fukushima T, Hara-Yamamura H, Nakashima K, Tan LC, Okabe S.  Multiple-endpoints gene alteration-based (MEGA) assay: A toxicogenomics approach for water quality assessment of wastewater effluents.  Chemosphere  2017  188:312-319  PubMed ID: 28888119
10488  Oda Y, Hidaka M, Suzuki A.  Caffeine Has a Synergistic Anticancer Effect with Cisplatin via Inhibiting Fanconi Anemia Group D2 Protein Monoubiquitination in Hepatocellular Carcinoma Cells.  Biol. Pharm. Bull.  2017  40:2005-2009  PubMed ID: 28855448
10490  Takano M, Kamei H, Nagahiro M, Kawami M, Yumoto R.  Nicotine transport in lung and non-lung epithelial cells.  Life Sci.  2017  188:76-82  PubMed ID: 28866099
10491  Inoue C, Zhao C, Tsuduki Y, Udono M, Wang L, Nomura M, Katakura Y.  SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes.  NPJ Aging Mech Dis  2017  3:11  PubMed ID: 28868154
10494  Toda M, Totoki T, Nakamura C, Yasuma T, D' Alessandro-Gabazza CN, Mifuji-Moroka R, Nishihama K, Iwasa M, Horiki N, Gabazza EC, Takei Y.  Low dose of alcohol attenuates pro-atherosclerotic activity of thrombin.  Atherosclerosis  2017  265:215-224  PubMed ID: 28923781
10495  Kawakami K, Moritani C, Uraji M, Fujita A, Kawakami K, Hatanaka T, Suzaki E, Tsuboi S.  Sake lees hydrolysate protects against acetaminophen-induced hepatotoxicity via activation of the Nrf2 antioxidant pathway.  J Clin Biochem Nutr  2017  61:203-209  PubMed ID: 29203962
10519  Zhou Y, Fukuda T, Hang Q, Hou S, Isaji T, Kameyama A, Gu J.  Inhibition of fucosylation by 2-fluorofucose suppresses human liver cancer HepG2 cell proliferation and migration as well as tumor formation.  Sci Rep  2017  7:11563  PubMed ID: 28912543
10531  Oura K, Tadokoro T, Fujihara S, Morishita A, Chiyo T, Samukawa E, Yamana Y, Fujita K, Sakamoto T, Nomura T, Yoneyama H, Kobara H, Mori H, Iwama H, Okano K, Suzuki Y, Masaki T.  Telmisartan inhibits hepatocellular carcinoma cell proliferation in vitro by inducing cell cycle arrest.  Oncol. Rep.  2017  38:2825-2835  PubMed ID: 29048654
10552  Okubo S, Uto T, Goto A, Tanaka H, Nishioku T, Yamada K, Shoyama Y.  Berberine Induces Apoptotic Cell Death via Activation of Caspase-3 and -8 in HL-60 Human Leukemia Cells: Nuclear Localization and Structure-Activity Relationships.  Am. J. Chin. Med.  2017  45:1497-1511  PubMed ID: 29025293
10622  Igarashi K, Yamashita K, Katoh H, Kojima K, Ooizumi Y, Nishizawa N, Nishiyama R, Kawamata H, Tajima H, Kaizu T, Kumamoto Y, Watanabe M.  Prognostic significance of promoter DNA hypermethylation of the cysteine dioxygenase 1 (CDO1) gene in primary gallbladder cancer and gallbladder disease.  PLoS ONE  2017  12:e0188178  PubMed ID: 29161283
10634  Hara J, Tanaka Y, Kaneko H, Itoh Y, Ikegaya H.  Detection of hepatitis B virus DNA and HBsAg from postmortem blood and bloodstains.  Arch. Virol.  2018  163:633-637  PubMed ID: 29198036
10641  Kaji K, Nishimura N, Seki K, Sato S, Saikawa S, Nakanishi K, Furukawa M, Kawaratani H, Kitade M, Moriya K, Namisaki T, Yoshiji H.  Sodium glucose cotransporter 2 inhibitor canagliflozin attenuates liver cancer cell growth and angiogenic activity by inhibiting glucose uptake.  Int. J. Cancer  2018  142:1712-1722  PubMed ID: 29205334
10660  Darwish WS, Ikenaka Y, Nakayama S, Mizukawa H, Thompson LA, Ishizuka M.  β-carotene and retinol reduce benzo[a]pyrene-induced mutagenicity and oxidative stress via transcriptional modulation of xenobiotic metabolizing enzymes in human HepG2 cell line.  Environ Sci Pollut Res Int  2018  25:6320-6328  PubMed ID: 29247416
10774  Yajima Y, Lee CN, Yamada M, Utoh R, Seki M.  Development of a perfusable 3D liver cell cultivation system via bundling-up assembly of cell-laden microfibers.  J. Biosci. Bioeng.  2018    PubMed ID: 29502942
10786  He Q, Okajima T, Onoe H, Subagyo A, Sueoka K, Kuribayashi-Shigetomi K.  Origami-based self-folding of co-cultured NIH/3T3 and HepG2 cells into 3D microstructures.  Sci Rep  2018  8:4556  PubMed ID: 29540810
10847  Kojima K, Nakamura T, Ohbu M, Katoh H, Ooizumi Y, Igarashi K, Ishii S, Tanaka T, Yokoi K, Nishizawa N, Yokota K, Kosaka Y, Sato T, Watanabe M, Yamashita K.  Cysteine dioxygenase type 1 (CDO1) gene promoter methylation during the adenoma-carcinoma sequence in colorectal cancer.  PLoS ONE  2018  13:e0194785  PubMed ID: 29746493
10930    Bioactive Compounds from the Leaves of Lumnitzera racemosa against Acetaminophen-induced Liver damage in vitro  Journal of Arid Land Studies  2016  26, 179 - 182 
10936    Cell Sheet Fabrication Using RGD Peptide-Coupled Alginate Hydrogels Fabricated by an Electrodeposition Method  Chem. Lett.  2017  46, 605–608 
10959    Induction of Drug Resistance in Human Hepatoma Cells Cultured on a Silicate Fiber-Based 3D Scaffold  Adv Biochem Biotechnol  2017  112 
10975    Collagen Microparticle-mediated 3D Cell Organization: A Facile Route to Bottom-Up Engineering of Thick and Porous Tissues  ACS Biomater. Sci. Eng.  2017  3, 2144–2154 
10985    Effects of Cytokines and Immunosuppressants on the Production of Serum Amyloid A Protein and C-reactive  Dokkyo Journal of Medical Sciences  2003  30(2):131-139 
10990    CYP3A4 induction mechanism of polycyclic aromatic hydrocarbons differs from that of rifampicin in PXR binding element  Fundamental Toxicological Sciences  2017  4, 229-239 
11025    Isolation of activating factors of serotonin N-acetyltransferase from rice peptides  Journal of Functional Foods  2018  41, 148-154 
11089  Yamamiya D, Mizukoshi E, Kaji K, Terashima T, Kitahara M, Yamashita T, Arai K, Fushimi K, Honda M, Kaneko S.  Immune responses of human T lymphocytes to novel hepatitis B virus-derived peptides.  PLoS ONE  2018    PubMed ID: 29856876
11139  Saikawa S, Kaji K, Nishimura N, Seki K, Sato S, Nakanishi K, Kitagawa K, Kawaratani H, Kitade M, Moriya K, Namisaki T, Mitoro A, Yoshiji H.  Angiotensin receptor blockade attenuates cholangiocarcinoma cell growth by inhibiting the oncogenic activity of Yes-associated protein.  Cancer Lett.  2018    PubMed ID: 30031758
11113  Kawai T, Ota N, Imasato A, Shirasaki Y, Otsuka K, Tanaka Y.  Profiling of N-linked glycans from 100 cells by capillary electrophoresis with large-volume dual preconcentration by isotachophoresis and stacking.  J Chromatogr A  2018    PubMed ID: 29945790
11160  Languon S, Tuffour I, Quayson EE, Appiah-Opong R, Quaye O.  In Vitro Evaluation of Cytotoxic Activities of Marketed Herbal Products in Ghana.  J Evid Based Integr Med  2018    PubMed ID: 30088418
11292  Hori T, Kurosawa O.  A Three-dimensional Cell Culture Method with a Micromesh Sheet and Its Application to Hepatic Cells.  Tissue Eng Part C Methods  2018    PubMed ID: 30412042
4452  Phoebe Trio1, Atsuyoshi Kawahara, Satoru Fujisaki, De-Xing Hou  Comparative Gene Expression Profiling in Cultured Cell Lines after Treatment with Wasabi-derived Isothiocyanates  KIMIKA  2018  29, 1-10 
4224  Sultana H, Watanabe K, Rana MM, Takashima R, Ohashi A, Komai M, Shirakawa H.  Effects of Vitamin K₂ on the Expression of Genes Involved in Bile Acid Synthesis and Glucose Homeostasis in Mice with Humanized PXR.  Nutrients  2018    PubMed ID: 30060524
4225  Takashima Y, Horisawa K, Udono M, Ohkawa Y, Suzuki A.  Prolonged inhibition of hepatocellular carcinoma cell proliferation by combinatorial expression of defined transcription factors.  Cancer Sci.  2018    PubMed ID: 30220099
4483  Anusornvongchai T, Nangaku M, Jao TM, Wu CH, Ishimoto Y, Maekawa H, Tanaka T, Shimizu A, Yamamoto M, Suzuki N, Sassa R, Inagi R.  Palmitate deranges erythropoietin production via transcription factor ATF4 activation of unfolded protein response.  Kidney Int.  2018  94(3):536-550  PubMed ID: 29887316
4484  Wang S, Fumoto S, Miyamoto H, Tanaka M, Nishida K.  Edaravone, a cytoprotective drug, enhances transgene expression mediated by lipoplexes in HepG2 cells and mice.  Int J Pharm  2018  548(1):173-181  PubMed ID: 29969708



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