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RCB1884 : H-111-TC
update : 2018/08/01
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| Comment | Human cell line derived from gastric cancer. TKG0411 (Deposited from Tohoku Univ.). Cell growth is slow. |
| Comment from the depositor | |
| Terms and conditions | Basically, there is no restriction regarding academic use. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Obinata, Masuo
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| Originator |
Kudo, T
|
| Year of deposit |
2004
|
| Original cell |
TKG0411
|
| Animal |
human
< Mammals
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| Genus |
Homo
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| Species |
sapiens
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| Tissue |
stomach
|
| Case history |
gastric cancer
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| Classification |
cancer
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| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0411）
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|
Lifespan |
infinite
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| Morphology |
epithelial-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Medium and additives |
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RPMI1640 + 10% FBS
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| Antibiotics |
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Free
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Passage method |
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(0.05% trypsin + 0.02% EDTA) or (0.25% trypsin + 0.02% EDTA)
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : once/2 weeks, Medium Renewal : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
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(-)
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| STR (human) |
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OK
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| Isozyme |
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LD, NP
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| Chromosome mode |
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51-64(50) : /57(6),58(9),59(9),60(14),61(3),62(3)/
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
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| User's Publication |
3
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| User's Publication |
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9978
Kneissl J, Hartmann A, Pfarr N, Erlmeier F, Lorber T, Keller S, Zwingenberger G, Weichert W, Luber B.
Influence of the HER receptor ligand system on sensitivity to cetuximab and trastuzumab in gastric cancer cell lines.
J. Cancer Res. Clin. Oncol.
2017
143:573-600
PubMed ID: 27933395
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10662
Miyamoto S, Nagamura Y, Nakabo A, Okabe A, Yanagihara K, Fukami K, Sakai R, Yamaguchi H.
Aberrant alternative splicing of RHOA is associated with loss of its expression and activity in diffuse-type gastric carcinoma cells.
Biochem. Biophys. Res. Commun.
2018
495:1942-1947
PubMed ID: 29247652
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4190
Kurata A, Yamada M, Ohno SI, Inoue S, Hashimoto H, Fujita K, Takanashi M, Kuroda M.
Expression level of microRNA-200c is associated with cell morphology in vitro and histological differentiation through regulation of ZEB1/2 and E-cadherin in gastric carcinoma.
Oncol. Rep.
2018
PubMed ID: 29138864
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