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RCB1882 : BALL-1  update : 2018/08/01
CommentHuman cell line derived from B cell leukemia. TKG0467 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Obinata, Masuo
Originator Miyoshi, I. & Hiraki, S.
Year of deposit 2004
Original cell TKG0467
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue blood
Case history acute lymphoblastoid leukemia (B cell leukemia)
Classification cancer
History Cell Resource Center for Biomedical Research, Tohoku University(TKG0467)
Lifespan infinite
Morphology lymphocyte-like
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) LT 400
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004
621  Miyoshi I, Hiraki S, Tsubota T, Kubonishi I, Matsuda Y, Nakayama T, Kishimoto H, Kimura I, Masuji H.  Human B cell, T cell and null cell leukaemic cell lines derived from acute lymphoblastic leukaemias.  Nature  1977  267(5614):843-4  PubMed ID: 197412

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