Cell No. : Cell Name RCB1879 : PEER  update : 2024/01/17
Comment			Human T cell line derived from acute T cell leukemia. TKG0377 (Deposited from Tohoku Univ.).
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Obinata, Masuo
		Originator	Ravid, Z.
		Year of deposit	2004
		Original cell	TKG0377
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Tissue	lymphoid,T-leukemia
		Classification	cancer
		History	Cell Resource Center for Biomedical Research, Tohoku University（TKG0377）
		Lifespan	infinite
		Morphology	other
		Cellosaurus(Expasy)	CVCL_1913
			deposit info	lot info
		Medium	Medium List
		Culture type		Suspension cells
		Culture medium		RPMI1640 + 10% FBS
		Culture method
		Antibiotics		Free
		Passage method		dilution
	Culture information	Passage ratio		1 : 10 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		LT 400
		Virus (HTLV-1)		(-)
		Isozyme		LD, NP
		Chromosome mode		88-92(50) : 88(6),89(8),90(16),91(14),92(6)
		STR(human)		OK
Reference information		Reference	2
		User's Publication	1

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Reference

5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898

2711  Ravid Z, Goldblum N, Zaizov R, Schlesinger M, Kertes T, Minowada J, Verbi W, Greaves M  Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype  Int J Cancer  1980  25:7055-710  PubMed ID: 14768698   DOI: 10.1002/ijc.2910250604

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User's Publication

5608  Zhou X, Koizumi Y, Zhang M, Natsui M, Koyota S, Yamada M, Kondo Y, Hamada F, Sugiyama T.  Cadmium-coordinated supramolecule suppresses tumor growth of T-cell leukemia in mice  Cancer Sci  2015  106(5):635-41  PubMed ID: 25735932   DOI: 10.1111/cas.12651