Cell No. : Cell Name
RCB1878 : RPMI1788
update : 2024/01/17
|
| Comment | IgM (lambda) secreting B cell line. Established from peripheral blood lymphocytes of apparently normal 33-y-o male. HLA profile: A2, Aw33, B7, B14. TKG0464 (Deposited from Tohoku Univ.). |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Obinata, Masuo
|
| Year of deposit |
2004
|
| Original cell |
TKG0464
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Male
|
| Age at sampling |
33 years
|
| Tissue |
peripheral blood
|
| Classification |
transformed
|
| History |
Cell Resource Center for Biomedical Research, Tohoku University（TKG0464）
|
|
Lifespan |
infinite
|
| Morphology |
lymphoblast-like
|
| Cellosaurus(Expasy) |
CVCL_2710
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Suspension cells
|
| Culture method |
|
General Guidelines for Culturing Suspension Cells, 浮遊細胞の培養に関する一般的な注意(Japanese)
|
| Culture medium |
|
RPMI1640 + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
Culture information
|
Passage ratio |
|
1 : 6 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
LT 400
|
| Virus (HTLV-1) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
47(50), 47,XY,+3
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
5
|
| User's Publication |
4
|
| Reference |
|
5042
Haruta Y, Seon BK.
Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.
Proc Natl Acad Sci U S A
1986
83(20):7898-902
PubMed ID: 3464004
DOI: 10.1073/pnas.83.20.7898
|
|
306
Minowada J, Nonoyama M, Moore GE, Rauch AM, Pagano JS
The presence of the Epstein-Barr viral genome in human lymphoblastoid B-cell lines and its absence in a myeloma cell line
Cancer Res
1974
34(8):1898-903
PubMed ID: 4366935
|
|
4986
Huang CC.
Effect on growth but not on chromosomes of the mammalian cells after treatment with three organophosphorus insecticides.
Proc Soc Exp Biol Med
1973
142(1):36-40
PubMed ID: 4683261
DOI: 10.3181/00379727-142-36952
|
|
2940
Huang CC, Moore GE
Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies.
J Natl Cancer Inst
1969
43:1119-28
PubMed ID: 5353241
|
|
2235
Moore GE.
Lymphoblastoid cell lines from normal persons and those with nonmalignant diseases
J Surg Res
1969
9(3):139-41
PubMed ID: 5774617
DOI: 10.1016/0022-4804(69)90044-4
|
| User's Publication |
|
9943
Okochi M, Koike S, Tanaka M, Honda H.
Detection of Her2-overexpressing cancer cells using keyhole shaped chamber array employing a magnetic droplet-handling system.
Biosens Bioelectron
2017
93:32-39
PubMed ID: 27866824
DOI: 10.1016/j.bios.2016.11.013
|
|
7357
Yamamoto S, Fei J, Okochi M, Shimizu K, Yusa A, Kondo N, Iwata H, Nakanishi H, Honda H.
Efficient capturing of circulating tumor cells using a magnetic capture column and a size-selective filter.
Bioprocess Biosyst Eng
2015
38:1693-704
PubMed ID: 25964183
DOI: 10.1007/s00449-015-1412-9
|
|
4990
Ukiya M, Kawaguchi T, Ishii K, Ogihara E, Tachi Y, Kurita M, Ezaki Y, Fukatsu M, Kushi Y, Akihisa T.
Cytotoxic activities of amino acid-conjugate derivatives of abietane-type diterpenoids against human cancer cell lines.
Chem Biodivers
2013
10(7):1260-8
PubMed ID: 23847070
DOI: 10.1002/cbdv.201300043
|
|
4994
Danjoh I, Shirota R, Hiroyama T, Nakamura Y.
Dominant expansion of a cryptic subclone with an abnormal karyotype in B lymphoblastoid cell lines during culture.
Cytogenet Genome Res
2013
139(2):88-96
PubMed ID: 23128794
DOI: 10.1159/000343757
|
Back
Top
CELL BANK Website