Cell No. : Cell Name RCB1870 : LY-A/hCERT  update : 2023/09/21
Comment			Subline of LY-A in which human CERT cDNA are expressed and trafficking of ceramide is recovered
Comment from the depositor
Terms and conditions			In publishing research results obtained by the use of the BIOLOGICAL RESOURCE, a citation of the literature ref. (J Biol Chem 1998;273:33787-33794, J Cell Biol 1999;144:673-685), Nature 2003;426:803-809) designated by the DEPOSITOR is required.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Hanada, Kentaro
		Originator	Hanada, kentaro
		Year of deposit	2004
		Original cell	CHO-K1
		Animal	_hamster < Mammals
		Strain name	Chinese
		Genus	Cricetulus
		Species	griseus
		Gender	Female
		Age at sampling	adult
		Tissue	ovary
		Classification	mutant
		Recombinant	recombinant
		Exogene	pcDNA/hCERT, neo
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_L555
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HamF12 + 10% NBS (or FBS)
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage cell No		1-5 x 10 5 cells/100 mm dish
		SC frequency		Subculture : once/week, Medium Renewal : 2 times/week
		Temperature		33 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	1

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Reference

2714  Hanada, Kentaro, Kumagai, Keigo, Yasuda, Satoshi, Miura, Yukiko, Kawano, Miyuki, Fukasawa, Masayoshi, Nishijima, Masahiro  Molecular machinery for non-vesicular trafficking of ceramide.  Nature  2003  426:803-9  PubMed ID: 14685229   DOI: 10.1038/nature02188

2713  Fukasawa M, Nishijima M, Hanada K  Genetic evidence for ATP-dependent endoplasmic reticulum-to-Golgi apparatus trafficking of ceramide for sphingomyelin synthesis in Chinese hamster ovary cells.  J Cell Biol  1999  144:673-85  PubMed ID: 10037789   DOI: 10.1083/jcb.144.4.673

2687  Hanada K, Hara T, Fukasawa M, Yamaji A, Umeda M, Nishijima M  Mammalian cell mutants resistant to a sphingomyelin-directed cytolysin. Genetic and biochemical evidence for complex formation of the LCB1 protein with the LCB2 protein for serine palmitoyltransferase.  J Biol Chem  1998  273:33787-94  PubMed ID: 9837968   DOI: 10.1074/jbc.273.50.33787

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User's Publication

18253  Elwell CA, Jiang S, Kim JH, Lee A, Wittmann T, Hanada K, Melancon P, Engel JN.  Chlamydia trachomatis co-opts GBF1 and CERT to acquire host sphingomyelin for distinct roles during intracellular development  PLoS Pathog  2011  7(9):e1002198  PubMed ID: 21909260   DOI: 10.1371/journal.ppat.1002198