Back Back
RCB1862 : NIH3T3-3-4  update : 2018/07/06
CommentSubclone of NIH3T3
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Todaro, G. J.
Year of deposit 2003
Original cell NIH3T3
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
History ref. RCB0150, subcloned by RCB.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 2
User's Publication 26

To topTop
298  Jainchill, J L, Aaronson, S A, Todaro, G J  Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells.  J Virol  1969  4:549-53  PubMed ID: 4311790
5004  Benveniste RE, Lieber MM, Todaro GJ.  A distinct class of inducible murine type-C viruses that replicates in the rabbit SIRC cell line.  Proc Natl Acad Sci U S A.  1974  71(3):602-6  PubMed ID: 4132531

To topTop
User's Publication
3385  Izumi, Nobuhiro, Mizuguchi, Shinjiro, Inagaki, Yutaka, Saika, Shizuya, Kawada, Norifumi, Nakajima, Yuji, Inoue, Kiyotoshi, Suehiro, Shigefumi, Friedman, Scott L, Ikeda, Kazuo  BMP-7 opposes TGF-beta1-mediated collagen induction in mouse pulmonary myofibroblasts through Id2.  Am J Physiol Lung Cell Mol Physiol  2006  290:L120-6  PubMed ID: 16126788
7008  Hayasaka N, Hirano A, Miyoshi Y, Tokuda IT, Yoshitane H, Matsuda J, Fukada Y.  Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein.  Elife  2017  6:  PubMed ID: 29227248
7052  Terajima H, Yoshitane H, Ozaki H, Suzuki Y, Shimba S, Kuroda S, Iwasaki W, Fukada Y.  ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm.  Nat. Genet.  2016  49:146-151  PubMed ID: 27893733
7559  Shiba K., Tagaya M., Sugiyama T., Hanagata N.  Preparation of luminescent titania/dye hybrid nanoparticles and their dissolution properties for controlling cellular environments.  RSC Adv  2015  5:104343-104353 
7739  Koike M, Yutoku Y, Koike A.  Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation.  Biochem. Biophys. Res. Commun.  2011  412:39-43  PubMed ID: 21787748
7769  Tamura K, Mizutani T, Haga H, Kawabata K.  Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy.  Acta Biomater  2011  7:3766-72  PubMed ID: 21704197
7776  Oike H, Kobori M, Suzuki T, Ishida N.  Caffeine lengthens circadian rhythms in mice.  Biochem. Biophys. Res. Commun.  2011  410:654-8  PubMed ID: 21684260
7829  Shoji K, Tsubaki M, Yamazoe Y, Satou T, Itoh T, Kidera Y, Tanimori Y, Yanae M, Matsuda H, Taga A, Nakamura H, Nishida S.  Mangiferin induces apoptosis by suppressing Bcl-xL and XIAP expressions and nuclear entry of NF-κB in HL-60 cells.  Arch. Pharm. Res.  2011  34:469-75  PubMed ID: 21547680
7836  Kitajima T, Obuse S, Adachi T, Tomita M, Ito Y.  Recombinant human gelatin substitute with photoreactive properties for cell culture and tissue engineering.  Biotechnol. Bioeng.  2011  108:2468-76  PubMed ID: 21538336
8075  Okada S, Nagai A, Oaki Y, Komotori J, Imai H.  Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals.  Acta Biomater  2010  7:1290-7  PubMed ID: 20965284
8151  Noguchi T, Michihata T, Nakamura W, Takumi T, Shimizu R, Yamamoto M, Ikeda M, Ohmiya Y, Nakajima Y.  Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes.  Biochemistry  2010  49:8053-61  PubMed ID: 20718447
8206  Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
8311  Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y.  Enhanced beetle luciferase for high-resolution bioluminescence imaging.  PLoS ONE  2010  5:e10011  PubMed ID: 20368807
8463  Li X, Nakajima Y, Niwa K, Viviani VR, Ohmiya Y.  Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging.  Protein Sci.  2009  19:26-33  PubMed ID: 19866487
8467  Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
8497  Niwa K, Nakajima Y, Ohmiya Y.  Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase.  Anal. Biochem.  2009  396:316-8  PubMed ID: 19748476
8650  Tokumoto Y, Horimoto K, Miyake J.  TRAIL inhibited the cyclic AMP responsible element mediated gene expression.  Biochem. Biophys. Res. Commun.  2009  381:533-6  PubMed ID: 19233138
8798  Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
8820  Zhao H, Sugawara T, Miura S, Iijima T, Kashimoto S.  Intrathecal landiolol inhibits nociception and spinal c-Fos expression in the mouse formalin test.  Can J Anaesth  2007  54:201-7  PubMed ID: 17331932
10385  Bay DH, Busch A, Lisdat F, Iida K, Ikebukuro K, Nagasawa K, Karube I, Yoshida W.  Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands.  BMC Mol. Biol.  2017  18:17  PubMed ID: 28655335
10783  Wu YK, Umeshima H, Kurisu J, Kengaku M.  Nesprins and opposing microtubule motors generate a point force that drives directional nuclear motion in migrating neurons.  Development  2018  145:  PubMed ID: 29519888
10803  Sugimoto M, Kitagawa Y, Yamada M, Yajima Y, Utoh R, Seki M.  Micropassage-embedding composite hydrogel fibers enable quantitative evaluation of cancer cell invasion under 3D coculture conditions.  Lab Chip  2018  18:1378-1387  PubMed ID: 29658964
11253  Kajikawa M, Ose T, Fukunaga Y, Okabe Y, Matsumoto N, Yonezawa K, Shimizu N, Kollnberger S, Kasahara M, Maenaka K.  Structure of MHC class I-like MILL2 reveals heparan-sulfate binding and interdomain flexibility.  Nat Commun  2018    PubMed ID: 30337538
4433    Scan-less confocal phase imaging based on dual-comb microscopy  Optica  2018  5, 634-643 
4434    Comparisons between silk fibroin nonwoven electrospun fabrics using aqueous and formic acid solutions  Journal International Journal of Polymeric Materials and Polymeric Biomaterials   2018  67 
4539  Yuki Kishimoto, Takanori Kobashi, Hideaki Morikawa and Yasushi Tamada  Production of three-dimensional silk fibroin nanofiber non-woven fabric by wet electrospinning  Nippon Silk Gakkaishi  2017  25, 49-57 

Back Back Return Top Page