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RCB1862 : NIH3T3-3-4  update : 2018/07/06
CommentSubclone of NIH3T3
Comment from the depositor
Restriction (Category)a
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Todaro, G. J.
Year of deposit 2003
Original cell NIH3T3
Animal mouse < Mammals
Strain name NIH Swiss
Age at sampling embryo
Tissue embryo
Classification other
History ref. RCB0150, subcloned by RCB.
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP (mouse) OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 0
User's Publication 21


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Reference

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User's Publication
Izumi, Nobuhiro, Mizuguchi, Shinjiro, Inagaki, Yutaka, Saika, Shizuya, Kawada, Norifumi, Nakajima, Yuji, Inoue, Kiyotoshi, Suehiro, Shigefumi, Friedman, Scott L, Ikeda, Kazuo  BMP-7 opposes TGF-beta1-mediated collagen induction in mouse pulmonary myofibroblasts through Id2.  Am J Physiol Lung Cell Mol Physiol  2006  290:L120-6  PubMed ID: 16126788
Hayasaka N, Hirano A, Miyoshi Y, Tokuda IT, Yoshitane H, Matsuda J, Fukada Y.  Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein.  Elife  2017  6:  PubMed ID: 29227248
Terajima H, Yoshitane H, Ozaki H, Suzuki Y, Shimba S, Kuroda S, Iwasaki W, Fukada Y.  ADARB1 catalyzes circadian A-to-I editing and regulates RNA rhythm.  Nat. Genet.  2016  49:146-151  PubMed ID: 27893733
Shiba K., Tagaya M., Sugiyama T., Hanagata N.  Preparation of luminescent titania/dye hybrid nanoparticles and their dissolution properties for controlling cellular environments.  RSC Adv  2015  5:104343-104353 
Tamura K, Mizutani T, Haga H, Kawabata K.  Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy.  Acta Biomater  2011  7:3766-72  PubMed ID: 21704197
Oike H, Kobori M, Suzuki T, Ishida N.  Caffeine lengthens circadian rhythms in mice.  Biochem. Biophys. Res. Commun.  2011  410:654-8  PubMed ID: 21684260
Shoji K, Tsubaki M, Yamazoe Y, Satou T, Itoh T, Kidera Y, Tanimori Y, Yanae M, Matsuda H, Taga A, Nakamura H, Nishida S.  Mangiferin induces apoptosis by suppressing Bcl-xL and XIAP expressions and nuclear entry of NF-κB in HL-60 cells.  Arch. Pharm. Res.  2011  34:469-75  PubMed ID: 21547680
Kitajima T, Obuse S, Adachi T, Tomita M, Ito Y.  Recombinant human gelatin substitute with photoreactive properties for cell culture and tissue engineering.  Biotechnol. Bioeng.  2011  108:2468-76  PubMed ID: 21538336
Okada S, Nagai A, Oaki Y, Komotori J, Imai H.  Control of cellular activity of fibroblasts on size-tuned fibrous hydroxyapatite nanocrystals.  Acta Biomater  2010  7:1290-7  PubMed ID: 20965284
Noguchi T, Michihata T, Nakamura W, Takumi T, Shimizu R, Yamamoto M, Ikeda M, Ohmiya Y, Nakajima Y.  Dual-color luciferase mouse directly demonstrates coupled expression of two clock genes.  Biochemistry  2010  49:8053-61  PubMed ID: 20718447
Matsubara S, Takahashi T, Kimura AP.  Epigenetic patterns at the mouse prolyl oligopeptidase gene locus suggest the CpG island in the gene body to be a novel regulator for gene expression.  Gene  2010  465:17-29  PubMed ID: 20600704
Nakajima Y, Yamazaki T, Nishii S, Noguchi T, Hoshino H, Niwa K, Viviani VR, Ohmiya Y.  Enhanced beetle luciferase for high-resolution bioluminescence imaging.  PLoS ONE  2010  5:e10011  PubMed ID: 20368807
Li X, Nakajima Y, Niwa K, Viviani VR, Ohmiya Y.  Enhanced red-emitting railroad worm luciferase for bioassays and bioimaging.  Protein Sci.  2009  19:26-33  PubMed ID: 19866487
Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
Niwa K, Nakajima Y, Ohmiya Y.  Applications of luciferin biosynthesis: Bioluminescence assays for l-cysteine and luciferase.  Anal. Biochem.  2009  396:316-8  PubMed ID: 19748476
Tokumoto Y, Horimoto K, Miyake J.  TRAIL inhibited the cyclic AMP responsible element mediated gene expression.  Biochem. Biophys. Res. Commun.  2009  381:533-6  PubMed ID: 19233138
Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
Zhao H, Sugawara T, Miura S, Iijima T, Kashimoto S.  Intrathecal landiolol inhibits nociception and spinal c-Fos expression in the mouse formalin test.  Can J Anaesth  2007  54:201-7  PubMed ID: 17331932
Bay DH, Busch A, Lisdat F, Iida K, Ikebukuro K, Nagasawa K, Karube I, Yoshida W.  Identification of G-quadruplex structures that possess transcriptional regulating functions in the Dele and Cdc6 CpG islands.  BMC Mol. Biol.  2017  18:17  PubMed ID: 28655335
Wu YK, Umeshima H, Kurisu J, Kengaku M.  Nesprins and opposing microtubule motors generate a point force that drives directional nuclear motion in migrating neurons.  Development  2018  145:  PubMed ID: 29519888
Kajikawa M, Ose T, Fukunaga Y, Okabe Y, Matsumoto N, Yonezawa K, Shimizu N, Kollnberger S, Kasahara M, Maenaka K.  Structure of MHC class I-like MILL2 reveals heparan-sulfate binding and interdomain flexibility.  Nat Commun  2018    PubMed ID: 30337538



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