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Cell No. : Cell Name
RCB1845 : ΔUbc9+cUbc9-DT40 update : 2024/01/17 |
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| Comment | Endogenous Ubc9 deficient DT40 cells but expressing Ubc9 exogenously | |||
| Comment from the depositor | ||||
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Seki, Masayuki | ||
| Originator | Hayashi, Tomoko | |||
| Year of deposit | 2002 | |||
| Animal | _avian < Birds | |||
| Tissue | B cell | |||
| Classification | mutant | |||
| Recombinant | recombinant | |||
| Exogene | Ubc9 | Lifespan | infinite | |
| Morphology | lymphocyte-like | |||
| Cellosaurus(Expasy) | CVCL_AS94 | |||
| Medium | Medium List | Culture type | Suspension cells | |
| Culture medium | RPMI1640 + 10% FBS + 1% Chicken serum | |||
| Culture method | ||||
| Antibiotics | Free | Passage method | dilution | Culture information | Passage cell No | 2 x 10 5 cells/ml |
| SC frequency | Subculture : every 2 days | |||
| Temperature | 39.5 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | 90% FBS + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Animal PCR | OK | |||
| Others | Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day. | |||
| Reference information | Reference | 2 | ||
| User's Publication | 0 | |||
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