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Cell No. : Cell Name
RCB1823 : CHO·1F8  update : 2023/01/05
CommentSubline of CHO-K1 that expresses human platelet activating factor receptor.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Depositor) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
The University of Tokyo, Graduate School of Medicine
Department of Biochemistry and Molecular Biology, Faculty of Medicine
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan
Dr.SHIMIZU Takao
Fax. +81-3-3813-8732
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Shimizu, Takao
Originator Shimizu, Takao
Year of deposit 2002
Original cell CHO-K1
Animal _hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification transformed
Recombinant recombinant
Exogene pcDNAIneo, human platelet activating factor (PAF) receptor cDNA
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_T032
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HamF12 + 10% FBS + 0.3mg/ml G418
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
2817  Aihara M, Ishii S, Kume K, Shimizu T  Interaction between neurone and microglia mediated by platelet-activating factor.  Genes Cells  2000  5:397-406  PubMed ID: 10886367   DOI: 10.1046/j.1365-2443.2000.00333.x
2816  Aoki Y, Nakamura M, Kodama H, Matsumoto T, Shimizu T, Noma M  A radioreceptor binding assay for platelet-activating factor (PAF) using membranes from CHO cells expressing human PAF receptor.  J Immunol Methods  1995  186:225-31  PubMed ID: 7594622   DOI: 10.1016/0022-1759(95)00147-3

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