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Cell No. : Cell Name
RCB1813 : ΔWRNΔBLM-DT40  update : 2024/01/17
CommentSubline of DT40 cell line, lacking both of WRN (Werner syndrome gene) and BLM (Bloom syndrome gene) expression.
Comment from the depositor
Terms and conditionsA prior written permission of the approver(Originator) is necessary for any kind of use including academic use and for-profit use.
Remarks
approver's address
×
English
Address
RIKEN Genomic Sciences Center
Protein Research Group
1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama City, Kanagawa, 230-0045 Japan
Dr.MATSUMOTO Takehisa
Fax. +81-45-503-9653
Order Form Order Form(C-0005.pdf)   Approval Form(C-0006.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Takeda, Shunichi
Originator Matsumoto, Takehisa
Year of deposit 2002
Original cell DT40
Animal _avian < Birds
Tissue B cell
Classification mutant
Recombinant recombinant
Exogene Blast, His, Puro, Geneticine(neo)
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2H96
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS + 1% Chicken serum + 50μM 2-Mercaptoethanol
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage cell No 1.5-2 x 10 5 cells/ml
Passage ratio 1 : 10-20 split
SC frequency Subculture : every 2 days
Temperature 39.5 ℃
CO2 concentration 5 %
Freeze medium 90% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Others Freezing : 1.0-1.5x10 7 cells/ml/vial. Thawing : 1ml of cells into 20ml media. It is unnecessary to remove DMSO. Pass the next day.
Reference information Reference 2
User's Publication 0


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Reference
17830  Hoa NN, Akagawa R, Yamasaki T, Hirota K, Sasa K, Natsume T, Kobayashi J, Sakuma T, Yamamoto T, Komatsu K, Kanemaki MT, Pommier Y, Takeda S, Sasanuma H.  Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines  Genes Cells  2015  20(12):1059-76  PubMed ID: 26525166   DOI: 10.1111/gtc.12310
17832  Imamura O, Fujita K, Itoh C, Takeda S, Furuichi Y, Matsumoto T.  Werner and Bloom helicases are involved in DNA repair in a complementary fashion  Oncogene  2002  21(6):954-63  PubMed ID: 11840341   DOI: 10.1038/sj.onc.1205143

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